15-deoxy-Delta 12, 14-PGJ2 ELISA Kit (Colorimetric) Summary
Standard Curve Range |
195 - 200,000 pg/ml |
Sensitivity |
36.8 pg/ml |
Assay Type |
Competitive ELISA |
Inter-Assay |
CV% < 14.4% |
Intra-Assay |
CV% < 6.2% |
Spike Recovery |
Tissue Culture Media - 97.4% Human Saliva - 94.4% Human Urine - 113.9% Porcine Plasma - 105.0% |
Sample Volume |
100 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Applications/Dilutions
Packaging, Storage & Formulations
Kit Components
Components
|
- Antibody
- Assay buffer
- concentrate
- Conjugate
- GxR IgG Microtiter plate
- pNpp Substrate
- Standard
- Stop solution
- Wash buffer
|
Alternate Names for 15-deoxy-Delta 12, 14-PGJ2 ELISA Kit (Colorimetric)
Background
15-deoxy-12,14-Prostaglandin J2 (15-d-PGJ2) is one of the ultimate dehydration products of PGD2, formed via the intermediate 12-PGJ2. 15-d-PGJ2 is physiologically present in body fluids at concentration of 10-12 to 10-9 M, but increases dramatically under stress such as infection and inflammation. 15-d-PGJ2 exerts both pro- and anti-inflammatory effects in a cell-type and concentration-dependent manner, with many of these effects mediated via binding to the isoform of the peroxisome proliferators-activated receptor (PPAR-y). 15-d-PGJ2 appears to induce HO-1 expression by a PPAR-y-independent mechanism mediated by the generation of reactive oxygen species (ROS).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for 15-deoxy-Delta 12, 14-PGJ2 ELISA Kit (NBP2-61285). (Showing 1 - 1 of 1 FAQ).
-
wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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