15-deoxy-Delta 12, 14-PGJ2 ELISA Kit (Colorimetric)

ELISA: 15-deoxy-Delta 12, 14-PGJ2 ELISA Kit (Colorimetric) [NBP2-61285] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

• Applications: ELISA
• Suitable Sample Type: Plasma, serum, and urine from any species.
• Standard Curve Range: 195 - 200,000 pg/ml
• Sensitivity: 36.8 pg/ml

15-deoxy-Delta 12, 14-PGJ2 ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 195 - 200,000 pg/ml
• Sensitivity: 36.8 pg/ml
• Assay Type: Competitive ELISA
• Inter-Assay: CV% < 14.4%
• Intra-Assay: CV% < 6.2%
• Spike Recovery: Tissue Culture Media - 97.4%
  Human Saliva - 94.4%
  Human Urine - 113.9%
  Porcine Plasma - 105.0%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Store at 4°C.

Background

15-deoxy-12,14-Prostaglandin J2 (15-d-PGJ2) is one of the ultimate dehydration products of PGD2, formed via the intermediate 12-PGJ2. 15-d-PGJ2 is physiologically present in body fluids at concentration of 10-12 to 10-9 M, but increases dramatically under stress such as infection and inflammation. 15-d-PGJ2 exerts both pro- and anti-inflammatory effects in a cell-type and concentration-dependent manner, with many of these effects mediated via binding to the isoform of the peroxisome proliferators-activated receptor (PPAR-y). 15-d-PGJ2 appears to induce HO-1 expression by a PPAR-y-independent mechanism mediated by the generation of reactive oxygen species (ROS).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.