13-HODE ELISA Kit (Colorimetric)

ELISA: 13-HODE ELISA Kit (Colorimetric) [NBP2-61283] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Discontinued

13-HODE ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 3.9 - 1,000 ng/ml
• Sensitivity: 1.6 ng/ml
• Assay Type: Competitive ELISA
• Inter-Assay: Mean 7.6%
• Intra-Assay: Mean 6.8%
• Spike Recovery: Tissue Culture Media - 101.6%
  Human Serum - 89.3%
  Human Saliva - 107%
  Human Urine - 115%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Store at 4°C.

Alternate Names for 13-HODE ELISA Kit (Colorimetric)

• (9Z,11E,13S)-13-Hydroxy-9,11-octadecadienoic acid
• 13(S)-HODE
• 13S-hydroxy-9Z,11E-octadecadienoic acid
• 13S-hydroxyoctadecadienoic acid

Background

The major human dietary poly-unsaturated fatty acid is linoleic acid, an essential fatty acid that can be metabolized to either 13-hydroxyoctadecadienoic acid [13(S)-HODE] via lipoxygenase or [13(R)-HODE] by p450 enzymes. Alternatively, cyclooxygenase metabolism of linoleic acid results in the production of hydroxyeicosatetraenoic acid [15(S)-HETE]. Changes in the intra-cellular ratio of 13(S)-HODE to 15(S)-HETE have been linked to a number of physiological conditions including cancer and cardiovascular disease. 13(S)-HODE helps to mediate cell adhesion and excessive proliferation that is critical in the formation of tumors and metastases, as well as the formation of atherogenic plaques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for 13-HODE ELISA Kit (NBP2-61283)(1)

Publication using NBP2-61283

• Kwong SC, Abd Jamil AH, Rhodes A et al. Fatty acid binding protein 7 mediates linoleic acid-induced cell death in triple negative breast cancer cells by modulating 13-HODE Biochimie 2020-09-12 [PMID: 32931863] (ELISA)

FAQs for 13-HODE ELISA Kit (NBP2-61283). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.