13-HODE ELISA Kit (Colorimetric) Summary
| Standard Curve Range |
3.9 to 1000 ng/mL |
| Sensitivity |
1.6 ng/mL |
| Assay Type |
Competitive ELISA |
| Inter-Assay |
CV% < 7.60% |
| Intra-Assay |
CV% < 6.83% |
| Spike Recovery |
Cell Culture Supernatant: 101.60%, Serum: 89.30%, Saliva: 107.00%, Urine: 115.00% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
13(S)-HODE ELISA Kit is a competitive immunoassay for the quantitative determination of free 13(S)-HODE in biological fluids. |
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- 13(S)-HODE ELISAAntibody
- 13(S)-HODE ELISAConjugate
- 13(S)-HODE Standard
- Assay Buffer
- Goat anti-Rabbit IgGMicrotiter Plate
- Plate Sealers
- pNpp Substrate
- Stop Solution
- Wash Buffer Concentrate
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for 13-HODE ELISA Kit (Colorimetric)
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for 13-HODE ELISA Kit (KA0317). (Showing 1 - 1 of 1 FAQ).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
