| Applications | ELISA |
| Suitable Sample Type | Plasma, culture supernates |
| Standard Curve Range | 195 - 50000 pg/ml |
| Sensitivity | 146 pg/ml |
| Description | Store all components at 4C, except standard and conjugate at -20C. |
| Specificity | Cross-reactivities for a number of related compounds: 12(R)-HETE: 2.5% 15-HETE: 0.3% 5(S)-HETE: 0.2% 8,15-diHETE: 0.1% 5,15-diHETE: 0.1% PGE2: 0.1% PGF2alpha: 0.1% PGD2: 0.1% 6-keto-PGF1alpha: 0.1% Thromboxane B2: 0.1% Arachidonic Acid: 0.1% Leukotriene B4: 0.1% Leukotriene C4: 0.1% Leukotriene D4: 0.1% Leukotriene E4: 0.1% 8-HETE: <0.1% 9-HETE: <0.1% 11-HETE: <0.1% |
| Standard Curve Range | 195 - 50000 pg/ml |
| Sensitivity | 146 pg/ml |
| Assay Type | Indirect ELISA |
| Inter-Assay | CV%: <21% |
| Intra-Assay | CV%: <15% |
| Spike Recovery | Culture Media: 94% Sodium heparin plasma 104 |
| Sample Volume | 100 ul |
| Kit Type | ELISA Kit (Colorimetric) |
| Storage | Storage of components varies. See protocol for specific instructions. |
| Components |
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