12S-HETE ELISA Kit (Colorimetric)

Non-species specific 12S-HETE ELISA Kit (Colorimetric) [NBP3-07908] - Standard curve

• Applications: ELISA
• Suitable Sample Type: Plasma, culture supernates
• Standard Curve Range: 195 - 50000 pg/ml
• Sensitivity: 146 pg/ml

12S-HETE ELISA Kit (Colorimetric) Summary

• Description: Store all components at 4C, except standard and conjugate at -20C.
• Specificity: Cross-reactivities for a number of related compounds:
  
  12(R)-HETE: 2.5%
  15-HETE: 0.3%
  5(S)-HETE: 0.2%
  8,15-diHETE: 0.1%
  5,15-diHETE: 0.1%
  PGE2: 0.1%
  PGF2alpha: 0.1%
  PGD2: 0.1%
  6-keto-PGF1alpha: 0.1%
  Thromboxane B2: 0.1%
  Arachidonic Acid: 0.1%
  Leukotriene B4: 0.1%
  Leukotriene C4: 0.1%
  Leukotriene D4: 0.1%
  Leukotriene E4: 0.1%
  8-HETE: <0.1%
  9-HETE: <0.1%
  11-HETE: <0.1%
• Standard Curve Range: 195 - 50000 pg/ml
• Sensitivity: 146 pg/ml
• Assay Type: Indirect ELISA
• Inter-Assay: CV%: <21%
• Intra-Assay: CV%: <15%
• Spike Recovery: Culture Media: 94%
  Sodium heparin plasma 104
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for 12S-HETE ELISA Kit (Colorimetric)

• (5Z,8Z,10E,12S,14Z)-12-hydroxyicosa-5,8,10,14-tetraenoic acid
• 12(S)-HETE
• 12(S)-Hydroxyeicosatetraenoic acid
• 12-Hete
• 12-Hydroxyeicosatetraenoic acid
• 12S-HETE
• 54397-83-0
• CHEBI:34146

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for 12S-HETE ELISA Kit (NBP3-07908). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.