12S-HETE ELISA Kit (Colorimetric)

Images

 
Non-species specific 12S-HETE ELISA Kit (Colorimetric) [NBP3-07908] - Standard curve

Product Details

Summary
Applications ELISA
Suitable Sample Type
Plasma, culture supernates
Standard Curve Range
195 - 50000 pg/ml
Sensitivity
146 pg/ml

Order Details

12S-HETE ELISA Kit (Colorimetric) Summary

Description
Store all components at 4C, except standard and conjugate at -20C.
Specificity
Cross-reactivities for a number of related compounds:

12(R)-HETE: 2.5%
15-HETE: 0.3%
5(S)-HETE: 0.2%
8,15-diHETE: 0.1%
5,15-diHETE: 0.1%
PGE2: 0.1%
PGF2alpha: 0.1%
PGD2: 0.1%
6-keto-PGF1alpha: 0.1%
Thromboxane B2: 0.1%
Arachidonic Acid: 0.1%
Leukotriene B4: 0.1%
Leukotriene C4: 0.1%
Leukotriene D4: 0.1%
Leukotriene E4: 0.1%
8-HETE: <0.1%
9-HETE: <0.1%
11-HETE: <0.1%
Standard Curve Range
195 - 50000 pg/ml
Sensitivity
146 pg/ml
Assay Type
Indirect ELISA
Inter-Assay
CV%: <21%
Intra-Assay
CV%: <15%
Spike Recovery
Culture Media: 94%
Sodium heparin plasma 104
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. 12(S)-HETE Antibody
  2. 12(S)-HETE Assay Layout Sheet
  3. 12(S)-HETE Conjugate
  4. 12(S)-HETE Standard
  5. Assay Buffer Concentrate
  6. Goat anti-Rabbit IgG Microtiter Plate
  7. Plate Sealer
  8. pNpp Substrate
  9. Stop Solution
  10. Wash Buffer Concentrate

Alternate Names for 12S-HETE ELISA Kit (Colorimetric)

  • (5Z,8Z,10E,12S,14Z)-12-hydroxyicosa-5,8,10,14-tetraenoic acid
  • 12(S)-HETE
  • 12(S)-Hydroxyeicosatetraenoic acid
  • 12-Hete
  • 12-Hydroxyeicosatetraenoic acid
  • 12S-HETE
  • 54397-83-0
  • CHEBI:34146

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for 12S-HETE ELISA Kit (NBP3-07908) (0)

There are no publications for 12S-HETE ELISA Kit (NBP3-07908).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for 12S-HETE ELISA Kit (NBP3-07908) (0)

There are no reviews for 12S-HETE ELISA Kit (NBP3-07908). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for 12S-HETE ELISA Kit (NBP3-07908). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Additional 12S-HETE Products

Blogs on 12S-HETE

There are no specific blogs for 12S-HETE, but you can read our latest blog posts.

Contact Information

Product PDFs

Review this Product

Be the first to review our 12S-HETE ELISA Kit (Colorimetric) and receive a gift card or discount.