12S-HETE ELISA Kit (Colorimetric)

ELISA: 12S-HETE ELISA Kit (Colorimetric) [NBP2-61258] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

• Applications: ELISA
• Suitable Sample Type: culture supernatants and plasma from any species Cited sample types include serum, renal interstitial fluid and tissue
• Standard Curve Range: 195 - 50,000 pg/ml
• Sensitivity: 146 pg/ml

12S-HETE ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 195 - 50,000 pg/ml
• Sensitivity: 146 pg/ml
• Assay Type: Competitive ELISA
• Inter-Assay: Mean 11.3%
• Intra-Assay: Mean 10.3%
• Spike Recovery: Culture Media - 94%
  Sodium Heparin Plasma - 104%
  EDTA Plasma - 97%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Store at 4°C.

Alternate Names for 12S-HETE ELISA Kit (Colorimetric)

• (5Z,8Z,10E,12S,14Z)-12-hydroxyicosa-5,8,10,14-tetraenoic acid
• 12(S)-HETE
• 12(S)-Hydroxyeicosatetraenoic acid
• 12-Hete
• 12-Hydroxyeicosatetraenoic acid
• 12S-HETE
• 54397-83-0
• CHEBI:34146

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for 12S-HETE ELISA Kit (NBP2-61258). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.