10X Tris-EDTA buffer pH 9.0

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Product Details

Summary
Applications IHC, IHC-P

Order Details

10X Tris-EDTA buffer pH 9.0 Summary

Specificity
10X Tris-EDTA Buffer for Heat Induced Epitope Recovery, pH 9.0

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
Application Notes
To recover antigens masked by fixation in cross linking fixatives such as formalin. 1. Deparaffinize and rehydrate tissue sections. 2. Fill a coplin jar with sufficient 1x Tris-EDTA Buffer to cover the tissue sections on the slides. 3. Place coplin jar in streamer or water bath. 4. Heat steamer or water bath containing coplin jar to 95-100C. 5. Place deparaffinized slides (1-3 slides/jar) in the coplin jar and incubate for 20-40 minutes (optimal incubation time should be determined by end user). 6. Remove coplin jar from the water bath and allow the slides to cool for 20 minutes to reach room temperature. 7. Wash slides in deionized water and then with wash buffer. Proceed with immunostaining.

Packaging, Storage & Formulations

Storage
Store at room temperature.
Buffer
Dilute one part buffer in nine parts distilled water.
Preservative
No Preservative

Background

In order to perform immunostaining, the tissue specimens should be fixed in appropriate fixative. The purpose of such fixative is to conserve the tissue from autolysis, to preserve tissue structures and to immobilize antigens. However, this requires harsh treatment of the antigens. As a result, antigens undergo chemical alteration of their primary, secondary and tertiary structures. Because of changes in the protein containing epitopes or in neighboring proteins, antigenic sites may be masked. In past, enzymatic treatment with proteolytic enzymes i.e. pepsin, trypsin or pronase has been performed to regain the masked antigens. Shi et al. (1991) have reported that the treatment of the tissue section with heavy metal solution in a microwave can regain the masked antigens significantly. However, heavy metals in the solution increase the risk of exposure of lab personnel to lead. To solve this problem, we have developed a new antigen unmasking solution which is free from heavy metals. Use of this antigen unmasker can prevent the risk of unnecessary exposure to the lab personnel and also resolve the handling and disposal problems.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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Product General Protocols

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  1. We are interesting to order the buffer 10x Tris-EDTA buffer pH9,cat No. NB900-62085.Is it consist only Tris and EDTA? If the answer is yes, what are the concentration of the Tris and EDTA?
    • Unfortunately we are unable to disclose the components of the buffer, since the information is proprietary.

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