10X Citrate Buffer pH 8.0 Summary
10x Citrate Buffer pH 8.0 for Heat Induced Epitope Recovery
- Immunohistochemistry 1:10-1:500
To recover antigens masked by fixation in cross linking fixatives such as formalin. 1. Deparaffinize and bring tissue section to buffer. 2. Fill the plastic coplin jar with the antigen unmasker solution. 3. Place the jar in the in steamer or water bath. 4. Preheat steamer or water bath containing coplin jars to 95-100 C. 5. Place the deparaffinized slides (1 to 3 slides/jar) in the coplin jar and incubate for 20-40 minutes (optimal incubation time should be determined by the end user). 6. Remove the coplin jars from the water bath and allow the slides to cool down for 20 minutes to reach to room temperature. 7. Wash the slides in de-ionized water and then with wash buffer and proceed for immunostaining.
Packaging, Storage & Formulations
Store at room temperature.
Dilute one part buffer with nine parts distilled water.
0.05% Sodium Azide
In order to perform immunostaining, the tissue specimens should be fixed in appropriate fixative. The purpose of such fixative is to conserve the tissue from autolysis, to preserve tissue structures and to immobilize antigens. However, this requires harsh treatment of the antigens. As a result, antigens undergo chemical alteration of their primary, secondary and tertiary structures. Because of changes in the protein containing epitopes or in neighboring proteins, antigenic sites may be masked. In past, enzymatic treatment with proteolytic enzymes i.e. pepsin, trypsin or pronase has been performed to regain the masked antigens. Shi et al. (1991) have reported that the treatment of the tissue section with heavy metal solution in a microwave can regain the masked antigens significantly. However, heavy metals in the solution increase the risk of exposure to lead for lab personnel. To solve this problem, we have developed a new antigen unmasking solution which is free from heavy metals. Use of this antigen unmasker can prevent the risk of unnecessary exposure for lab personnel and also resolve the handling and disposal problem.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed
for 6 months from date of receipt.
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