TNF-alpha Antibodies

510-RT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002) . Cytotoxicity elicited by Recombinant Rat TNF-a (0.025 ng/mL) is neutralized (green line) by increasing concentrations of Rat TNF-a Recombinant Monoclonal Antibody (Catalog # MAB510R). The ND₅₀ is typically 10-40 µg/mL in the presence of the metabolic inhibitor Actinomycin D (0.5 µg/mL, Catalog # 1229)." class="big_lightbox"> 510-RT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002) . Cytotoxicity elicited by Recombinant Rat TNF-a (0.025 ng/mL) is neutralized (green line) by increasing concentrations of Rat TNF-a Recombinant Monoclonal Antibody (Catalog # MAB510R). The ND₅₀ is typically 10-40 µg/mL in the presence of the metabolic inhibitor Actinomycin D (0.5 µg/mL, Catalog # 1229)." alt="Recombinant Rat TNF-a (Catalog # 510-RT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002) . Cytotoxicity elicited by Recombinant Rat TNF-a (0.025 ng/mL) is neutralized (green line) by increasing concentrations of Rat TNF-a Recombinant Monoclonal Antibody (Catalog # MAB510R). The ND₅₀ is typically 10-40 µg/mL in the presence of the metabolic inhibitor Actinomycin D (0.5 µg/mL, Catalog # 1229)." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="TNF-a was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Recombinant Mouse Anti-Human TNF-a Monoclonal Antibody (Catalog # MAB2101R) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

210-TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Human TNF-a (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Recombinant Human TNF-a Monoclonal Antibody (Catalog # MAB2101R). The ND₅₀ is typically 0.01-0.06 µg/mL in the presence of the metabolic inhibitor actinomycin D (0.5 µg/mL)." class="big_lightbox"> 210-TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Human TNF-a (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Recombinant Human TNF-a Monoclonal Antibody (Catalog # MAB2101R). The ND₅₀ is typically 0.01-0.06 µg/mL in the presence of the metabolic inhibitor actinomycin D (0.5 µg/mL)." class="big_thumb" />

5035-TG) was serially diluted and captured by Mouse Anti-Guinea Pig TNF‑ alpha Monoclonal Antibody (Catalog # MAB5035) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Guinea Pig TNF‑ alpha Biotinylated Monoclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 5035-TG) was serially diluted and captured by Mouse Anti-Guinea Pig TNF‑ alpha Monoclonal Antibody (Catalog # MAB5035) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Guinea Pig TNF‑ alpha Biotinylated Monoclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Guinea Pig TNF‑ alpha (Catalog # 5035-TG) was serially diluted and captured by Mouse Anti-Guinea Pig TNF‑ alpha Monoclonal Antibody (Catalog # MAB5035) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Guinea Pig TNF‑ alpha Biotinylated Monoclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

210-TA) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Cytotoxicity elicited by Recombinant Human TNF-a (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TNF-a Recombinant Monoclonal Antibody (Catalog # MAB610R). The ND₅₀ is typically 0.01-0.04 µg/mL in the presence of the metabolic inhibitor actinomycin D." class="big_lightbox"> 210-TA) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Cytotoxicity elicited by Recombinant Human TNF-a (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TNF-a Recombinant Monoclonal Antibody (Catalog # MAB610R). The ND₅₀ is typically 0.01-0.04 µg/mL in the presence of the metabolic inhibitor actinomycin D." alt="Recombinant Human TNF-a (Catalog # 210-TA) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Cytotoxicity elicited by Recombinant Human TNF-a (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TNF-a Recombinant Monoclonal Antibody (Catalog # MAB610R). The ND₅₀ is typically 0.01-0.04 µg/mL in the presence of the metabolic inhibitor actinomycin D." class="big_thumb" />

1011-CR) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Cotton Rat TNF‑a (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat TNF‑a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1011). The ND₅₀ is typically 0.01-0.05 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." class="big_lightbox"> 1011-CR) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Cotton Rat TNF‑a (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat TNF‑a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1011). The ND₅₀ is typically 0.01-0.05 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." alt="Recombinant Cotton Rat TNF‑a (Catalog # 1011-CR) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Cotton Rat TNF‑a (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat TNF‑a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1011). The ND₅₀ is typically 0.01-0.05 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL)." class="big_thumb" />