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![Western Blot: PKC Alpha, Beta, Gamma Antibody (9O5J1) [NBP3-33469] - Western blot analysis of various lysates, using PKC Alpha, Beta, Gamma Rabbit mAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilutionLysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.](http://images.novusbio.com/fullsize/nbp3-33469_rabbit-pkc-alpha-beta-gamma-mab-9o5j1-101220241243921.jpg "Western Blot: PKC Alpha, Beta, Gamma Antibody (9O5J1) [NBP3-33469] - Western blot analysis of various lysates, using PKC Alpha, Beta, Gamma Rabbit mAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilutionLysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.")
![Immunocytochemistry/Immunofluorescence: PKC alpha [p Ser657, p Tyr658] Antibody [NBP3-23303] - Immunocytochemical labeling of PKC phosphorylation in aldehyde-fixed and NP-40-permeabilized NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-PKC alpha (Ser-657/Tyr-658) (NBP3-23303) antibody in the absence (Left) or presence (Right) of blocking peptide. The antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23303_rabbit-pkc-alpha-p-ser657-p-tyr658-pab-19920231759448.jpg "Immunocytochemistry/Immunofluorescence: PKC alpha [p Ser657, p Tyr658] Antibody [NBP3-23303] - Immunocytochemical labeling of PKC phosphorylation in aldehyde-fixed and NP-40-permeabilized NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-PKC alpha (Ser-657/Tyr-658) (NBP3-23303) antibody in the absence (Left) or presence (Right) of blocking peptide. The antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.")
![Western Blot: PKC alpha [p Ser657, p Tyr658] Antibody [NBP3-23303] - Western blot analysis of immunoprecipitates from neonatal rat brain lysate using anti-PKC alpha antibody. Control and alkaline phosphatase treated precipitates were probed with anti-PKC alpha (Central region) or anti-phospho-PKC alpha (Ser-657/Tyr-658). The latter shows no detection of PKC alpha after phosphatase treatment.](http://images.novusbio.com/fullsize/nbp3-23303_rabbit-pkc-alpha-p-ser657-p-tyr658-pab-19920231856932.jpg)