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We offer Occludin Antibodies for use in common research applications: ELISA, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Proximity Ligation Assay, Sandwich ELISA, Western Blot. Each Occludin Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Occludin Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the Occludin Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Occludin polyclonal antibodies and browse our Occludin monoclonal antibody catalog.
![Western Blot: Occludin Antibody [NBP1-59435] - Sample Tissue: Human Fetal Liver Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/Occludin-Antibody-Western-Blot-NBP1-59435-img0003.jpg "Western Blot: Occludin Antibody [NBP1-59435] - Sample Tissue: Human Fetal Liver Antibody Dilution: 1.0 ug/ml")
![Western Blot: Occludin Antibody [NBP1-59435] - COLO205 cells lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/Occludin-Antibody-Western-Blot-NBP1-59435-img0002.jpg)
![Immunohistochemistry: Occludin Antibody (8H8) [NBP3-26449] - Image of Occludin Antibody (8H8) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26449_rabbit-occludin-mab-8h8-262202414225210.jpg "Immunohistochemistry: Occludin Antibody (8H8) [NBP3-26449] - Image of Occludin Antibody (8H8) diluted at 1:100 and staining in paraffin-embedded human colon cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
![Flow Cytometry: Occludin Antibody (8H8) [NBP3-26449] - Overlay Peak curve showing HepG2 cells stained with Occludin Antibody (8H8) (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26449_rabbit-occludin-mab-8h8-282202414183673.jpg)
![Immunoprecipitation: Occludin Antibody [NBP3-14709] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from 293T cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-Occludinrecombinant monoclonal antibody used for IP at 20 ul/mg lysate. Occludin was alsoimmunoprecipitated by rabbit anti-Occludin antibody. For blotting immunoprecipitated Occludin, was used at 1:1000. Chemiluminescence withan exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Occludin-Antibody-Immunoprecipitation-NBP3-14709-img0001.jpg "Immunoprecipitation: Occludin Antibody [NBP3-14709] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from 293T cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-Occludinrecombinant monoclonal antibody used for IP at 20 ul/mg lysate. Occludin was alsoimmunoprecipitated by rabbit anti-Occludin antibody. For blotting immunoprecipitated Occludin, was used at 1:1000. Chemiluminescence withan exposure time of 10 seconds.")
