IL-6 Antibodies

F0101B) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P). Quadrant markers were set based on control antibody staining (Catalog # MAB002). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_lightbox"> F0101B) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P). Quadrant markers were set based on control antibody staining (Catalog # MAB002). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." alt="Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated overnight with 250 ng/mL LPS were stained with Mouse Anti-Human IL-6 Monoclonal Antibody (Catalog # MAB2062) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P). Quadrant markers were set based on control antibody staining (Catalog # MAB002). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_thumb" />

561-R6) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Cotton Rat IL-6 (0.1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Cotton Rat IL-6 Monoclonal Antibody (Catalog # MAB561). The ND₅₀ is typically 3-15 µg/mL." class="big_lightbox"> 561-R6) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Cotton Rat IL-6 (0.1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Cotton Rat IL-6 Monoclonal Antibody (Catalog # MAB561). The ND₅₀ is typically 3-15 µg/mL." alt="Recombinant Cotton Rat IL-6 (Catalog # 561-R6) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Cotton Rat IL-6 (0.1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Cotton Rat IL-6 Monoclonal Antibody (Catalog # MAB561). The ND₅₀ is typically 3-15 µg/mL." class="big_thumb" />

NL004) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL004) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="IL‑6 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with PHA (positive staining) and untreated PBMCs (negative control) using Rabbit Anti-Human IL‑6 Monoclonal Antibody (Catalog # MAB95402) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

DY990). Mouse Anti-Human IL-6 Monoclonal Antibody (Catalog # MAB2063) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> DY990). Mouse Anti-Human IL-6 Monoclonal Antibody (Catalog # MAB2063) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant human IL-6 protein was serially diluted 2-fold and captured by Mouse Anti-Human IL-6 Monoclonal Antibody (Catalog # MAB9540) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human IL-6 Monoclonal Antibody (Catalog # MAB2063) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />

206-IL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-6 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Primate IL-6 Monoclonal Antibody (Catalog # MAB206R). The ND₅₀ is typically 0.05-0.15 µg/mL." class="big_lightbox"> 206-IL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-6 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Primate IL-6 Monoclonal Antibody (Catalog # MAB206R). The ND₅₀ is typically 0.05-0.15 µg/mL." alt="Recombinant Human IL-6 (206-IL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-6 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Primate IL-6 Monoclonal Antibody (Catalog # MAB206R). The ND₅₀ is typically 0.05-0.15 µg/mL." class="big_thumb" />

1609-CL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL-6 (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Canine IL-6 Monoclonal Antibody (Catalog # MAB1609). The ND₅₀ is typically 10-30 µg/mL." class="big_lightbox"> 1609-CL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL-6 (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Canine IL-6 Monoclonal Antibody (Catalog # MAB1609). The ND₅₀ is typically 10-30 µg/mL." alt="Recombinant Canine IL-6 (Catalog # 1609-CL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL-6 (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Canine IL-6 Monoclonal Antibody (Catalog # MAB1609). The ND₅₀ is typically 10-30 µg/mL." class="big_thumb" />

1609-CL) induces proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Canine IL-6 (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Canine IL-6 Monoclonal Antibody (Catalog # MAB16092). The ND₅₀ is typically 1-5 µg/mL." class="big_lightbox"> 1609-CL) induces proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Canine IL-6 (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Canine IL-6 Monoclonal Antibody (Catalog # MAB16092). The ND₅₀ is typically 1-5 µg/mL." alt="Recombinant Canine IL-6 (Catalog # 1609-CL) induces proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Canine IL-6 (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Canine IL-6 Monoclonal Antibody (Catalog # MAB16092). The ND₅₀ is typically 1-5 µg/mL." class="big_thumb" />

MAB9540) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human IL-6 Monoclonal Antibody (Catalog # MAB2063) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> MAB9540) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human IL-6 Monoclonal Antibody (Catalog # MAB2063) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant human IL-6 protein was serially diluted 2-fold and captured by Mouse Anti-Human IL-6 Monoclonal Antibody (Catalog # MAB9540) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human IL-6 Monoclonal Antibody (Catalog # MAB2063) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />

DY990). Rat Anti-Porcine IL-6 Monoclonal Antibody (Catalog # MAB6863) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> DY990). Rat Anti-Porcine IL-6 Monoclonal Antibody (Catalog # MAB6863) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Porcine IL-6 protein was serially diluted 2-fold and captured by Rat Anti-Porcine IL-6 Monoclonal Antibody (Catalog # MAB6862) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Porcine IL-6 Monoclonal Antibody (Catalog # MAB6863) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />

MAB6862) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Porcine IL-6 Monoclonal Antibody (Catalog # MAB6863) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> MAB6862) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Porcine IL-6 Monoclonal Antibody (Catalog # MAB6863) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Porcine IL-6 protein was serially diluted 2-fold and captured by Rat Anti-Porcine IL-6 Monoclonal Antibody (Catalog # MAB6862) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Porcine IL-6 Monoclonal Antibody (Catalog # MAB6863) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />