HIF-1 alpha Antibodies
We offer HIF-1 alpha antibodies that have been cited in 1,750+ publications with 110+ customer reviews. Browse our extensive catalog for anti-HIF-1 alpha polyclonal antibodies, monoclonal antibodies, and recombinant rabbit monoclonal antibodies. Validated research applications for HIF1a antibodies include Chromatin Immunoprecipitation (ChIP), ELISA, Flow Cytometry, Knockout Validated ICC, IHC, IHC Whole-Mount, IHC-Frozen, IHC-Paraffin, Immunofluorescence, IP, Ligand Activation, Proximity Ligation Assay, Simple Western, Tissue Culture Substratum, and Knockout Validated Western Blot. Our HIF1a antibodies can be used in a variety of model species: Human, Mouse, Rat, Avian, Bovine, Canine, Chicken, Ferret, Goat, Guinea Pig, Hamster, Monkey, Porcine, Primate, Rabbit, Sheep, and Xenopus. Choose the anti-HIF-1 alpha antibody best suited for your research needs by viewing the technical details, images, references, reviews and related products. Each HIF-1 alpha antibody is fully covered by our Guarantee+.
Alternate Names for HIF-1 alpha Antibodies
anti-HIF-1 alpha antibody, anti-HIF1A antibody, anti-ARNT-interacting protein antibody, anti-Basic-helix-loop-helix-PAS protein MOP1 antibody, anti-BHLHE78 antibody, anti-Class E basic helix-loop-helix protein 78 antibody, anti-HIF-1-alpha antibody, anti-HIF1-alpha antibody, anti-hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcriptionfactor) antibody, anti-hypoxia-inducible factor 1-alpha antibody, anti-Member of PAS protein 1 antibody, anti-member of PAS superfamily 1 antibody, anti-MOP1HIF1-ALPHA antibody, anti-PAS domain-containing protein 8 antibody, anti-PASD8alpha subunit (basic helix-loop-helix transcriptionfactor) antibody
27 Results for "hif-1-alpha" in Primary Antibodies
![Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (HA111) [NB100-296] - HIF-1 antibody was tested in human ovarian cancer tumor xenograft using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-HA111-Immunohistochemistry-Paraffin-NB100-296-img0001.jpg "Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (HA111) [NB100-296] - HIF-1 antibody was tested in human ovarian cancer tumor xenograft using DAB with hematoxylin counterstain.")
![Western Blot: HIF-1 alpha Antibody (HA111) [NB100-296] - Detection of HIF-1 alpha (125-130 kDa) from human placental villous explant total protein.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-HA111-Western-Blot-NB100-296-img0004.jpg)
![Western Blot: HIF-1 alpha [Hydroxy Pro564] Antibody [NB110-74679] - Detection (left panel) of HIF-1 alpha [Hydroxy Pro564] in nuclear extracts of A549 cells treated with MG132 (a proteasome inhibitor). Hydroxyproline is not recognized on HIF-1alpha when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation. Control staining is shown (right panel) using conventional anti-HIF-1alpha. The asterisk marks a band approximately 110 kDa molecular weight in size corresponding to HIF-1 alpha. The primary antibody was used at a 1:1000 dilution in 2% BLOTTO.](http://images.novusbio.com/fullsize/HIF-1-alpha-[Hydroxy-Pro564]-Antibody-Western-Blot-NB110-74679-img0004.jpg "Western Blot: HIF-1 alpha [Hydroxy Pro564] Antibody [NB110-74679] - Detection (left panel) of HIF-1 alpha [Hydroxy Pro564] in nuclear extracts of A549 cells treated with MG132 (a proteasome inhibitor). Hydroxyproline is not recognized on HIF-1alpha when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation. Control staining is shown (right panel) using conventional anti-HIF-1alpha. The asterisk marks a band approximately 110 kDa molecular weight in size corresponding to HIF-1 alpha. The primary antibody was used at a 1:1000 dilution in 2% BLOTTO.")
![Western Blot: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Western-Blot-NBP2-80765-img0001.jpg "Western Blot: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Immunofluorescent staining of HIF-1 alpha using catalog number NBP2-75977 (clone 2443B). Staining was observed in Hela cells treated with DFO but not in untreated Hela cells. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80765-img0002.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - HIF-1 alpha (clone 2443C) was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (Catalog # NBP2-75978) at 1.0 ug/mL for 1 hour at room temperature followed by incubation with the](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80765-img0003.jpg)
![Simple Western: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Lane view shows a specific band for HIF-1 alpha in HepG2 +/- DFO cell lysate using 20 ug/mL. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 116 KDa. Image from an internal validation. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Simple-Western-NBP2-80765-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (Catalog # NBP2-75978) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha at approximately 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Knockout-Validated-NBP2-80765-img0004.jpg)
![Western Blot: HIF-1 alpha Antibody - Exon 9 [NBP1-47178] - Human recombinant HIF-1 alpha protein.](http://images.novusbio.com/fullsize/HIF-1-alpha-Exon-9-Antibody-exon-9-Western-Blot-NBP1-47178-img0002.jpg "Western Blot: HIF-1 alpha Antibody - Exon 9 [NBP1-47178] - Human recombinant HIF-1 alpha protein.")
![Western Blot: HIF-1 alpha Antibody - Exon 13 [NBP1-47181] - Human recombinant HIF-1 alpha protein.](http://images.novusbio.com/fullsize/HIF-1-alpha-[exon-13]-Antibody-Western-Blot-NBP1-47181-img0002.jpg "Western Blot: HIF-1 alpha Antibody - Exon 13 [NBP1-47181] - Human recombinant HIF-1 alpha protein.")
![Western Blot: HIF-1 alpha Antibody (H1alpha 67-7) [NB100-130] - HIF-1 alpha in 1: Cos7 CoCl2 treated cells. 2: Untreated cells.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-H1alpha-67-7-Western-Blot-NB100-130-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody (H1alpha 67-7) [NB100-130] - HIF-1 alpha in 1: Cos7 CoCl2 treated cells. 2: Untreated cells.")
![Immunoprecipitation: HIF-1 alpha [Dimethyl Lys674] Antibody [NBP2-69089] - Parental or G9a KO HeLa cells were exposed to 1% O2 for 6 h, and subjected to IP with antibodies against K674me1 or K674me2, followed by immunoblotassays with antibodies against HIF-1 alpha, G9a or actin. Image courtesy of Weibo Luo, UT Southwestern.](http://images.novusbio.com/fullsize/HIF-1-alpha-[Dimethyl-Lys674]-Antibody-Immunoprecipitation-NBP2-69089-img0002.jpg "Immunoprecipitation: HIF-1 alpha [Dimethyl Lys674] Antibody [NBP2-69089] - Parental or G9a KO HeLa cells were exposed to 1% O2 for 6 h, and subjected to IP with antibodies against K674me1 or K674me2, followed by immunoblotassays with antibodies against HIF-1 alpha, G9a or actin. Image courtesy of Weibo Luo, UT Southwestern.")
![Immunoprecipitation: HIF-1 alpha [Monomethyl Lys674] Antibody [NBP2-69088] - Parental or G9a KO HeLa cells were exposed to 1% O2 for 6 h, and subjected to immunoprecipitation with antibodies against K674me1 or K674me2, followed by immunoblot assays with antibodies against HIF-1 alpha, G9a or actin. (Image courtesy of Weibo Luo, UT Southwestern).](http://images.novusbio.com/fullsize/HIF-1-alpha-[Monomethyl-Lys674]-Antibody-Immunoprecipitation-NBP2-69088-img0001.jpg "Immunoprecipitation: HIF-1 alpha [Monomethyl Lys674] Antibody [NBP2-69088] - Parental or G9a KO HeLa cells were exposed to 1% O2 for 6 h, and subjected to immunoprecipitation with antibodies against K674me1 or K674me2, followed by immunoblot assays with antibodies against HIF-1 alpha, G9a or actin. (Image courtesy of Weibo Luo, UT Southwestern).")
![Western Blot: HIF-1 alpha Antibody - Exon 12 [NBP1-47180] - Human recombinant HIF-1 alpha protein.](http://images.novusbio.com/fullsize/HIF-1-alpha-Exon-12-Antibody-exon-12-Western-Blot-NBP1-47180-img0003.jpg "Western Blot: HIF-1 alpha Antibody - Exon 12 [NBP1-47180] - Human recombinant HIF-1 alpha protein.")
![Western Blot: HIF-1 alpha Antibody - Exon 10 [NBP1-47179] - Human recombinant HIF-1 alpha protein](http://images.novusbio.com/fullsize/HIF-1-alpha-Exon-10-Antibody-exon-10-Western-Blot-NBP1-47179-img0002.jpg "Western Blot: HIF-1 alpha Antibody - Exon 10 [NBP1-47179] - Human recombinant HIF-1 alpha protein")
![Western Blot: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 250uM CoCl2 for 6 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HepG2 treated with 250uM CoCl2 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 93 kDa Observed band size: 120 kDa Exposure time: 1minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-278202415212466.jpg "Western Blot: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 250uM CoCl2 for 6 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HepG2 treated with 250uM CoCl2 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 93 kDa Observed band size: 120 kDa Exposure time: 1minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32424) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-2982024149260.jpg)
![Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Immunocytochemistry analysis of HeLa cells treated with or without 500μM CoCl2 for 24 hours labeling HIF-1 alpha with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-29202410334071.jpg)
