| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27734-100ul | |||
Immunoblot Buffer Group 1." alt="Western blot shows lysates ofSf 21 S. frugiperdainsect ovarian cell line mock transfected or transfected GST tagged Parkin. PVDF membrane was probed with 1 µg/mL of Hamster Anti-Glutathione S‑Transferase/GST Monoclonal Antibody (Catalog # MAB94991) followed by HRP-conjugated Anti-Hamster IgG Secondary Antibody. A specific band was detected for Glutathione S‑Transferase/GST tagged Parkin at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB94991-SP | |||
HAF018). A specific band was detected for Glutathione S-Transferase/GST as indicated. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of GST tagged Ubiquitin, GST tagged ISG-15, GST tagged FLAG®, HEK293T human embryonic kidney cell line,E. colitransfected with GST tagged BRDG1-SH2, andSf21 transfected GST tagged PARK1. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Glutathione S-Transferase/GST Monoclonal Antibody (Catalog # MAB9499) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Glutathione S-Transferase/GST as indicated. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB9499-SP | |||
![Western Blot: Glutathione S-Transferase/GST Antibody (SR3947) [NBP3-27734] - Western blot analysis of PC3 cell lysate.](http://images.novusbio.com/fullsize/nbp3-27734_rabbit-glutathione-s-transferase-gst-mab-sr3947-7520249522023.jpg "Western Blot: Glutathione S-Transferase/GST Antibody (SR3947) [NBP3-27734] - Western blot analysis of PC3 cell lysate.")
![Western Blot: Glutathione S-Transferase/GST Antibody (GST13) - VHH [NBP3-12166] - Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: Recombinant GST (0.2ug) [+]. Lane 3: HEK293T WCL / rec. GST(10ug/0.2ug) [+]. Lane 4: HEK293T Whole Cell Lysate (10ug) [-]. Lane 5: E.coli Host Cell Protein (HCP) Control Protein (10ug) [-]. Primary Antibody: Anti-GST (VhH) at 5.0ug/mL overnight at 2-8 degrees C. Secondary Antibody: Mouse Anti-6X His Tag Peroxidase at 1:3,000 for 30 mins at RT. Block: BlockOut Buffer. Exposure: 60 sec. Predicted MW: ~25.5kDa. Observed MW: ~25.5kDa. Unspecific Bands: ~70kDa minor background due to unspecific cross-reactivity of anti-6X His ab with unknown proteins of HEK293T lysate.](http://images.novusbio.com/fullsize/Glutathione-S-Transferase-GST-Antibody-GST13-VHH-Western-Blot-NBP3-12166-img0001.jpg "Western Blot: Glutathione S-Transferase/GST Antibody (GST13) - VHH [NBP3-12166] - Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: Recombinant GST (0.2ug) [+]. Lane 3: HEK293T WCL / rec. GST(10ug/0.2ug) [+]. Lane 4: HEK293T Whole Cell Lysate (10ug) [-]. Lane 5: E.coli Host Cell Protein (HCP) Control Protein (10ug) [-]. Primary Antibody: Anti-GST (VhH) at 5.0ug/mL overnight at 2-8 degrees C. Secondary Antibody: Mouse Anti-6X His Tag Peroxidase at 1:3,000 for 30 mins at RT. Block: BlockOut Buffer. Exposure: 60 sec. Predicted MW: ~25.5kDa. Observed MW: ~25.5kDa. Unspecific Bands: ~70kDa minor background due to unspecific cross-reactivity of anti-6X His ab with unknown proteins of HEK293T lysate.")
![Dot Blot: Glutathione S-Transferase/GST Antibody (GST13) - VHH [NBP3-12166] - Row A: Recombinant GST protein. Amount Dotted: (1) 200, (2) 100, (3) 50, (4) 25, (5) 12.5ng. Primary Antibody: 1.0ug/mL for 1.5hr at RT. Secondary Antibody: Mouse Anti-6X His Tag Peroxidase at 1:500 for 30 mins at RT. Block: BlockOut Buffer.](http://images.novusbio.com/fullsize/Glutathione-S-Transferase-GST-Antibody-GST13-VHH-Dot-Blot-NBP3-12166-img0002.jpg)
Western Blot Buffer Group 1." class="big_thumb" />
![Western Blot: Glutathione S-Transferase/GST Antibody (4D12.H7.D10.E3) [NBP2-44182] - Western Blot of Mouse anti-GSTS1 Monoclonal Antibody. Lane 1: rGSTS1 protein. Lane 2: GST. Load: 50 ng per lane. Primary antibody: Mouse anti-GSTS1 Monoclonal Antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase conjugated Rb-a-Ms IgG at 1:40,000 for 30 min at RT. Block: 0.75% Casein-TBS for 30 min at RT. Predicted/Observed size: 27 kDa, 27 kDa for GSTS1.](http://images.novusbio.com/fullsize/Glutathione-S-Transferase-GST-Antibody-Western-Blot-NBP2-44182-img0003.jpg "Western Blot: Glutathione S-Transferase/GST Antibody (4D12.H7.D10.E3) [NBP2-44182] - Western Blot of Mouse anti-GSTS1 Monoclonal Antibody. Lane 1: rGSTS1 protein. Lane 2: GST. Load: 50 ng per lane. Primary antibody: Mouse anti-GSTS1 Monoclonal Antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase conjugated Rb-a-Ms IgG at 1:40,000 for 30 min at RT. Block: 0.75% Casein-TBS for 30 min at RT. Predicted/Observed size: 27 kDa, 27 kDa for GSTS1.")
![Western Blot: Glutathione S-Transferase/GST Antibody (C11) [NBP3-42071] - Four different Recombinant proteins with Glutathione S-Transferase/GST Tag (Lane 1-4); Primary Ab: 0.5ug/ml NBP3-42071; Second Ab: 0.2ug/mL HRP-conjugated Goat Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-42071_mouse-glutathione-s-transferase-gst-mab-c11-1412025151384.jpg "Western Blot: Glutathione S-Transferase/GST Antibody (C11) [NBP3-42071] - Four different Recombinant proteins with Glutathione S-Transferase/GST Tag (Lane 1-4); Primary Ab: 0.5ug/ml NBP3-42071; Second Ab: 0.2ug/mL HRP-conjugated Goat Anti-Mouse IgG Polyclonal Antibody")
