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We offer CD133 Antibodies for use in common research applications: Flow (Intracellular), Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA. Each CD133 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our CD133 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the CD133 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our CD133 polyclonal antibodies and browse our CD133 monoclonal antibody catalog.
![Western Blot: CD133 Antibody (A8C3-R) [NBP3-32124] - Western blot analysis of CD133 on different lysates with Mouse anti-CD133 antibody (NBP3-32124) at 1/5,000 dilution. Lane 1: HT-29 cell lysate Lane 2: SW480 cell lysate Lane 3: NCCIT cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 97 kDa Observed band size: 120 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32124) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32124_mouse-cd133-mab-a8c3-r-27820241631670.jpg)
![Immunohistochemistry: CD133 Antibody (A8C3-R) [NBP3-32124] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Mouse anti-CD133 antibody (NBP3-32124) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32124) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32124_mouse-cd133-mab-a8c3-r-298202414161867.jpg)
![Western Blot: CD133 Antibody (HL1271) - Azide and BSA Free [NBP3-25403] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD133 antibody [HL1271] (NBP3-25403) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25403_rabbit-cd133-mab-hl1271-azide-and-bsa-free-2120249342744.jpg "Western Blot: CD133 Antibody (HL1271) - Azide and BSA Free [NBP3-25403] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD133 antibody [HL1271] (NBP3-25403) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: CD133 Antibody (HL1271) - Azide and BSA Free [NBP3-25403] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD133 antibody [HL1271] (NBP3-25403) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25403_rabbit-cd133-mab-hl1271-azide-and-bsa-free-2120249374967.jpg)
![Flow Cytometry: CD133 Antibody (W6B3C1) [NBP3-09159] - Analysis (surface staining) of human peripheral whole blood showing CD133 positive stem cells (blue) and lymphocytes (red) stained using anti-CD133 (293C3) purified, GAM-FITC.](http://images.novusbio.com/fullsize/CD133-Antibody-W6B3C1-Flow-Cytometry-NBP3-09159-img0001.jpg "Flow Cytometry: CD133 Antibody (W6B3C1) [NBP3-09159] - Analysis (surface staining) of human peripheral whole blood showing CD133 positive stem cells (blue) and lymphocytes (red) stained using anti-CD133 (293C3) purified, GAM-FITC.")
![Western Blot: CD133 Antibody (SR1137) [NBP3-22443] - Western blot analysis using NBP3-22443 in HT-29 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22443_rabbit-cd133-mab-sr1137-57202316112177.jpg "Western Blot: CD133 Antibody (SR1137) [NBP3-22443] - Western blot analysis using NBP3-22443 in HT-29 cell lysate.")
![ELISA: CD133 Antibody (5F5) [NBP3-26366] - The Binding Activity of CD133 with CD133 Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized CD133 at 5 ug/mL can bind CD133 Antibody, the EC50 is 0.4322-0.7189 ng/mL.](http://images.novusbio.com/fullsize/nbp3-26366_human-cd133-mab-5f5-26220241236350.jpg "ELISA: CD133 Antibody (5F5) [NBP3-26366] - The Binding Activity of CD133 with CD133 Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized CD133 at 5 ug/mL can bind CD133 Antibody, the EC50 is 0.4322-0.7189 ng/mL.")