CCL3/MIP-1 alpha Antibodies

HAF018). A specific band was detected for CCL3/MIP-1a at approximately 8 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for CCL3/MIP-1a at approximately 8 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of NR8383 rat alveolar macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 4 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Rat CCL3/MIP-1a Monoclonal Antibody (Catalog # MAB6625) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CCL3/MIP-1a at approximately 8 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF007). A specific band was detected for CCL3/MIP-1a at approximately 5 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 10." class="big_lightbox"> HAF007). A specific band was detected for CCL3/MIP-1a at approximately 5 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 10." alt="Western blot shows lysates of A-72 canine fibroma cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Canine CCL3/MIP-1a Monoclonal Antibody (Catalog # MAB7370) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CCL3/MIP-1a at approximately 5 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 10." class="big_thumb" />

NL007) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membranes of epithelial cells in convoluted tubules. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_lightbox"> NL007) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membranes of epithelial cells in convoluted tubules. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." alt="CCL3/MIP-1a was detected in perfusion fixed frozen sections of rat kidney using Mouse Anti-Rat CCL3/MIP-1a Monoclonal Antibody (Catalog # MAB66251) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membranes of epithelial cells in convoluted tubules. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" />

450-MA) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL3/MIP-1a (10 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Mouse CCL3/MIP-1a Monoclonal Antibody (Catalog # MAB4503). The ND₅₀ is typically 0.02-0.2 µg/mL." class="big_lightbox"> 450-MA) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL3/MIP-1a (10 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Mouse CCL3/MIP-1a Monoclonal Antibody (Catalog # MAB4503). The ND₅₀ is typically 0.02-0.2 µg/mL." alt="Recombinant Mouse CCL3/MIP-1a (Catalog # 450-MA) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL3/MIP-1a (10 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Mouse CCL3/MIP-1a Monoclonal Antibody (Catalog # MAB4503). The ND₅₀ is typically 0.02-0.2 µg/mL." class="big_thumb" />