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![Immunoprecipitation:Adenosine Deaminase/ADA AntibodyNBP3-30187] -Detection of human ADA by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ADA antibody NBP3-30187 (lot NBP3-30187-1) used for IP at 6 µg per reaction. ADA was also immunoprecipitated by a rabbit anti-ADA antibodies . For blotting immunoprecipitated ADA, NBP3-30187 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/nbp3-30187_rabbit-adenosine-deaminase-ada-pab-30122024138245.jpg "Immunoprecipitation:Adenosine Deaminase/ADA AntibodyNBP3-30187] -Detection of human ADA by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ADA antibody NBP3-30187 (lot NBP3-30187-1) used for IP at 6 µg per reaction. ADA was also immunoprecipitated by a rabbit anti-ADA antibodies . For blotting immunoprecipitated ADA, NBP3-30187 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")