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Spindle Checkpoint Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Spindle Checkpoint Pathway and Malignant Neoplasms, Neoplasms, Aneuploidy, Cell Transformation, Neoplastic, Genomic Instability. The study of the Spindle Checkpoint Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Spindle Checkpoint Pathway has been researched in relation to Mitosis, Anaphase, Cell Cycle, Chromosome Segregation, Metaphase. The Spindle Checkpoint Pathway complements our catalog of research reagents including antibodies and ELISA kits against MAD2L1, MXI1, BUB1B, APC, CDC20.

Spindle Checkpoint Bioinformatics Tool

Laverne is a handy bioinformatics tool to help facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Explore more on Spindle Checkpoint below! For more information on how to use Laverne, please read the How to Guide.
Vizit™, under license from BioVista Inc.

Top Research Reagents

We have 1430 products for the study of the Spindle Checkpoint Pathway that can be applied to Chromatin Immunoprecipitation, Western Blot, Flow Cytometry, Chromatin Immunoprecipitation (ChIP), Immunohistochemistry, Immunocytochemistry/Immunofluorescence from our catalog of antibodies and ELISA kits.

NB100-563
Immunohistochemistry: MAD2L1 Antibody [NB100-563] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- MAD2 used at a dilution of 1:1,000 (1ug/ml). Detection: DABImmunoprecipitation: MAD2L1 Antibody [NB100-563] - Detection of human MAD2L1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MAD2L1 antibody NB100-563 (lot 3) used for IP at 3 ug/mg lysate. MAD2L1 was also immunoprecipitated by a previous lot (lot 2) and by rabbit anti-MAD2L1 antibody NB100-564. Detection: Chemiluminescence with an exposure time of 30 seconds.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

     1 Review

14 Publications
NBP1-88626
Western Blot: Mxi1 Antibody [NBP1-88626] - Analysis in control (vector only transfected HEK293T lysate) and MXI1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunohistochemistry-Paraffin: Mxi1 Antibody [NBP1-88626] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.

Rabbit Polyclonal
Species Human
Applications IHC, IHC-P

NB100-353
Western Blot: BubR1 Antibody (8G1) [NB100-353] - BUBR1 detected in HeLa cell lysate using NB 100-353.Immunocytochemistry/Immunofluorescence: BubR1 Antibody (8G1) [NB100-353] - Immunofluorescence of an asynchronous cycling population of human cells (U2OS) with NB100-353. No signal is detected from interphase cells, whereas cells undergoing mitosis accumulate BubR1 at the kinetochores. Image reveals kinetochores at prometaphase. Image courtesy of Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK.

Mouse Monoclonal
Species Human, Mouse
Applications WB, Flow, ICC/IF

8 Publications
NB100-91662
Western Blot: APC Antibody [NB100-91662] - Lane1:Hela cell lysate.  Lane2:HEK293T cell lysate.  Lane3:Rat testis tissue lysate.  Immunocytochemistry/Immunofluorescence: APC Antibody [NB100-91662] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-APC Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

NB100-59828
Western Blot: Cdc20 Antibody [NB100-59828] - Detection of Human and Mouse CDC20 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDC20 antibody NB100-59828 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Immunohistochemistry-Paraffin: Cdc20 Antibody [NB100-59828] -   Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CDC20  used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB

Rabbit Polyclonal
Species Human, Mouse
Applications WB, IHC, IHC-P

4 Publications
H00000699-D01P
Western Blot: Bub1 Antibody [H00000699-D01P] - Analysis of BUB1 expression in mouse testis.Immunocytochemistry/Immunofluorescence: Bub1 Antibody [H00000699-D01P] - Analysis of antibody to BUB1 on HeLa cell. Antibody concentration 10 ug/ml.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, PLA

1 Publication
NBP2-66987
Immunocytochemistry/Immunofluorescence: Coagulation Factor XIV/Protein C Antibody (JM93-61) [NBP2-66987] - Staining Protein C in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Immunohistochemistry-Paraffin: Coagulation Factor XIV/Protein C Antibody (JM93-61) [NBP2-66987] - Analysis of paraffin-embedded human liver tissue using anti-Protein C antibody. Counter stained with hematoxylin.

Rabbit Monoclonal
Species Human
Applications WB, ICC/IF, IHC

NBP1-32775
Western Blot: MAD1L1/MAD1 Antibody [NBP1-32775] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAD1 antibody diluted by 1:5000.Immunocytochemistry/Immunofluorescence: MAD1L1/MAD1 Antibody [NBP1-32775] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MAD1 protein stained by MAD1 antibody diluted at 1:500. Red: HEC1, a kinetochore marker, stained by HEC1 antibody [9G3.23] diluted at 1:500. Blue: Hoechst 33342 staining.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ChIP, ICC/IF

2 Publications
NBP1-89979
Immunohistochemistry-Paraffin: Mad Antibody [NBP1-89979] - Staining in human bone marrow and liver tissues using anti-MXD1 antibody. Corresponding MXD1 RNA-seq data are presented for the same tissues.Immunohistochemistry-Paraffin: Mad Antibody [NBP1-89979] - Staining of human bone marrow shows high expression.

Rabbit Polyclonal
Species Human
Applications IHC, IHC-P

NB100-56651
Western Blot: PLK1 Antibody [NB100-56651] - Analysis of HCT-116 lysate using PLK1 antibody at 1:2000.Immunohistochemistry-Paraffin: PLK1 Antibody [NB100-56651] - Analysis of human placenta using PLK1 antibody at 1:100.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

1 Publication
MAB6028
Western blot shows lysates of A431 human epithelial carcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Human TTK Monoclonal Antibody (Catalog # MAB6028) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

Mouse Monoclonal
Species Human
Applications WB

     2 Reviews

NB200-103
Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-p53 (PAb 240) [NB200-103] at a 1:200 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, Flow

     3 Reviews

27 Publications
NBP1-88517
Western Blot: Bub3 Antibody [NBP1-88517] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-PPIB.Immunocytochemistry/Immunofluorescence: Bub3 Antibody [NBP1-88517] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC

1 Publication
NBP2-44687
Immunocytochemistry/Immunofluorescence: Cyclin B1 Antibody (CCNB1/1098) [NBP2-44687] - MCF-7 cells stained with CF555 Cyclin B1 Antibody (CCNB1/1098) (nuclei/nucleoli, red) and CF488A phalloidin (actin filaments, green). DNA is stained with Hoechst (blue).Immunohistochemistry-Paraffin: Cyclin B1 Antibody (CCNB1/1098) [NBP2-44687] - Human Tonsil stained with Cyclin B1 Monoclonal Antibody (CCNB1/1098)

Mouse Monoclonal
Species Human, Mouse
Applications Flow, ICC/IF, IHC

     1 Review

NB100-1103
Western Blot: Ribosomal protein S27 Antibody [NB100-1103] - Staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry-Paraffin: Ribosomal protein S27 Antibody [NB100-1103] -  Staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Goat Polyclonal
Species Human
Applications WB, IHC, IHC-P

1 Publication
NBP1-85729
Western Blot: CDC2/CDK1 Antibody [NBP1-85729] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.Immunocytochemistry/Immunofluorescence: CDC2/CDK1 Antibody [NBP1-85729] - Staining of human cell line U-251 MG shows localization to nucleus & cytosol. Antibody staining is shown in green.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

3 Publications
NB100-338
Western Blot: NDC80 Antibody (9G3.23) [NB100-338] - HEC1 antibody [9G3.23] detects HEC1 protein by western blot analysis. Various whole cell extracts and HeLa nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HEC1 antibody [9G3.23]  diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-193821) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate. Immunocytochemistry/Immunofluorescence: NDC80 Antibody (9G3.23) [NB100-338] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Hec1 stained by Hec1 antibody [9G3.23] diluted at 1:500. Red: alpha Tubulin 4a, a cytoskeleton marker, stained by alpha Tubulin 4a antibody diluted at 1:500.Blue: Hoechst 33342 staining.

Mouse Monoclonal
Species Human, Hamster
Applications WB, Flow, ICC/IF

     5 Reviews

45 Publications
NB100-1768
Western Blot: Chk1 Antibody (2G1D5) [NB100-1768] - Analysis using Chk1 mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Chk1 cDNA (2).Immunocytochemistry/Immunofluorescence: Chk1 Antibody (2G1D5) [NB100-1768] - Chk1 antibody was tested at 1:10 in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40X.

Mouse Monoclonal
Species Human
Applications WB, ICC/IF, IHC

NBP2-75659
Western Blot: Securin Antibody (JB37-37) [NBP2-75659] - Analysis of Securin on SiHa cell lysate using anti-Securin antibody at 1/500 dilution.Immunocytochemistry/Immunofluorescence: Securin Antibody (JB37-37) [NBP2-75659] - Staining Securin in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Rabbit Monoclonal
Species Human
Applications WB, Flow, ICC/IF