Submit your image related to Pathways to be featured!

Get Social

Submit your Twitter account related to Methylation to be featured!

Blogs

Submit your blog on Methylation to be featured!

Events

Submit your event on Methylation to be featured!

Videos

Submit your video on Methylation to be featured!

Charities

Submit your charity on Methylation to be featured!

Methylation Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Methylation Pathway and Malignant Neoplasms, Neoplasms, Carcinoma, Carcinogenesis, Cell Transformation, Neoplastic. The study of the Methylation Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Methylation Pathway has been researched in relation to Dna Methylation, Demethylation, Gene Silencing, Pathogenesis, Cell Cycle. The Methylation Pathway complements our catalog of research reagents including antibodies and ELISA kits against HISTONE H3, HISTONE H3, APC, APC, CDH1.

Methylation Bioinformatics Tool

Laverne is a handy bioinformatics tool to help facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Explore more on Methylation below! For more information on how to use Laverne, please read the How to Guide.
Vizit™, under license from BioVista Inc.

Top Research Reagents

We have 2297 products for the study of the Methylation Pathway that can be applied to Western Blot, Chromatin Immunoprecipitation, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Chromatin Immunoprecipitation (ChIP) from our catalog of antibodies and ELISA kits.

NB200-111
Western Blot: p14ARF/CDKN2A Antibody [NB200-111] - ARF expression levels in OCI/AML3 cells after proteasome treatment. This image was submitted via customer Review.Immunocytochemistry/Immunofluorescence: p14ARF/CDKN2A Antibody [NB200-111] - p14ARF antibody was tested in HeLa cells with Dylight 488 (green). Nuclei were counterstained with DAPI (blue). Tubulin was stained with alpha tubulin (red).

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ELISA, Flow

     2 Reviews

16 Publications
NB100-56519
Western Blot: DNMT1 Antibody (60B1220.1) [NB100-56519] - analysis of Dnmt1 in 2102EP (human embryonic carcinoma) in the A) absence and B) presence of immunizing peptide, recombinant human Dnmt1 protein in the C) absence and D) presence of immunizing peptide, NIH 3T3 (mouse embryonic fribroblast) in the E) absence and F) presence of immunizing peptide, and D3 (mouse embryonic stem cell) in the G) absence and H) presence of immunizing peptide using Dnmt1 antibody. this antibody. 5 ug/ml for testing the human cell line and 0.1 ug/ml was used for testing the recombinant protein and the mouse cell lines. ’s goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.Immunocytochemistry/Immunofluorescence: DNMT1 Antibody (60B1220.1) [NB100-56519] - Dnmt1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). An antibody dilution of 1:10 was used. Image objective 40x.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, ChIP

     2 Reviews

109 Publications
NB200-103
Western Blot: p53 Antibody (PAb 240) [NB200-103] - WB analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-p53 (PAb 240) [NB200-103] at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, Flow

     2 Reviews

22 Publications
NBP2-67381
Western Blot: MLH1 Antibody (SP08-04) [NBP2-67381] - Analysis of MLH1 on A431 cells lysates using anti-MLH1 antibody at 1/1,000 dilution.Immunocytochemistry/Immunofluorescence: MLH1 Antibody (SP08-04) [NBP2-67381] - Staining MLH1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

NB100-168
Western Blot: MGMT Antibody (MT 23.2) [NB100-168] - Analysis of MGMT (MT 23.2) antibody (NB100-168) - Detection of MGMT in MCF7 lysate using NB100-168 at 1:1000 dilution.Immunocytochemistry/Immunofluorescence: MGMT Antibody (MT 23.2) [NB100-168] - Detection of MGMT (Green) in Hela cells using NB100-168 at a 1:50 dilution. Nuclei (Blue) are counterstained using Hoechst 33258.

Mouse Monoclonal
Species Human, Mouse (Negative)
Applications WB, Simple Western, Flow

16 Publications
NBP2-03644
Western Blot: RASSF1 Antibody (2B11) [NBP2-03644] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RASSF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RASSF1.Immunocytochemistry/Immunofluorescence: RASSF1 Antibody (2B11) [NBP2-03644] Staining of COS7 cells transiently transfected by pCMV6-ENTRY RASSF1.

Mouse Monoclonal
Species Human
Applications WB, Flow, ICC/IF

1 Publication
NBP1-92025
Western Blot: Invadolysin Antibody [NBP1-92025] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).Immunocytochemistry/Immunofluorescence: Invadolysin Antibody [NBP1-92025] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol & focal adhesion sites. Antibody staining is shown in green.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

352-MS/CF


Species Human

8 Publications
NBP2-02450
Western Blot: Prostate Secretory Protein/PSP Antibody (6C7) [NBP2-02450] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Prostate Secretory Protein/PSP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Prostate Secretory Protein/PSP.Immunocytochemistry/Immunofluorescence: Prostate Secretory Protein/PSP Antibody (6C7) [NBP2-02450] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Prostate Secretory Protein/PSP.

Mouse Monoclonal
Species Human
Applications WB, ICC/IF, IHC

NBP2-15142
Western Blot: TMPRSS13 Antibody [NBP2-15142] - Sample (30 ug of whole cell lysate) A: PC-3 7.5% SDS PAGE; antibody diluted at 1:5000.Immunohistochemistry-Paraffin: TMPRSS13 Antibody [NBP2-15142] - Paraffin-embedded Breast carcinoma, using antibody at 1:250 dilution.

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

292-G2
1 μg/lane of Recombinant Human IGF-II was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 7 kDa.Recombinant Human IGF-II (Catalog # 292‑G2) stimulates cell proliferation in the MCF-7 human breast cancer cell line. The ED<SUB>50</SUB> for this effect is 1.5‑6 ng/mL.


Species Human

     1 Review

29 Publications
AF748
    Western  blot shows lysates of A431 human epithelial carcinoma cell line, A549 human  lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, P19  mouse embryonal carcinoma cell line, and 4T1 mouse breast cancer cell line.  PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse  E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody  (Catalog # <a class=    Simple  Western lane view shows lysates of 4T1 mouse breast cancer cell line, P19  mouse embryonal carcinoma cell line, A431 human epithelial carcinoma cell  line, and MCF‑7 human breast cancer cell line, loaded at  0.2 mg/mL. A specific band was detected for E‑Cadherin at  approximately 128 kDa (as indicated) using 5 µg/mL of Goat  Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal  Antibody (Catalog # AF748) followed by 1:50 dilution of HRP-conjugated  Anti-Goat IgG Secondary Antibody (Catalog # <a class=

Goat Polyclonal
Species Human, Mouse
Applications WB, Simple Western, Flow

     5 Reviews

23 Publications
NBP2-45603
Western Blot: p15INK4b/CDKN2B Antibody (3B6) [NBP2-45603] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN2B.Immunohistochemistry: p15INK4b/CDKN2B Antibody (3B6) [NBP2-45603] - Analysis of Adenocarcinoma of Human endometrium tissue.

Mouse monoclonal
Species Human
Applications WB, Flow, IHC

NB100-91662
Western Blot: APC Antibody [NB100-91662] - Lane1:Hela cell lysate.  Lane2:HEK293T cell lysate.  Lane3:Rat testis tissue lysate.  Immunohistochemistry-Paraffin: APC Antibody [NB100-91662] - IHC-P analysis of a formalin fixed paraffin embedded tissue section of human colon cancer xenograft using 1: 50 dilution of APC antibody.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

NBP2-52487
Immunohistochemistry: H4/n Antibody (3E7D9) [NBP2-52487] - Analysis of paraffin-embedded esophageal cancer tissues using HIST2H4A(20Me) mouse mAb with DAB staining.Immunohistochemistry: H4/n Antibody (3E7D9) [NBP2-52487] - Analysis of paraffin-embedded colon cancer tissues using HIST2H4A(20Me) mouse mAb with DAB staining.

Mouse Monoclonal
Species Human
Applications WB, ELISA, Flow

NBP2-07996
Western Blot: HIST1H4F Overexpression Lysate (Adult Normal) [NBP2-07996] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIST1H4F.


Species Human

NBP1-82454
Western Blot: SUB1 Antibody [NBP1-82454] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.Immunocytochemistry/Immunofluorescence: SUB1 Antibody [NBP1-82454] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & nucleoli.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

2 Publications
NBP2-37607
Western Blot: MTHFR Antibody (5D3) [NBP2-37607] - Western blot analysis using MTHFR mouse mAb against Rat Heart cell lysate.Immunohistochemistry: MTHFR Antibody (5D3) [NBP2-37607] - Immunohistochemical analysis of paraffin-embedded lung cancer using MTHFR mouse mAb with DAB staining.

Mouse Monoclonal
Species Human, Rat
Applications WB, ELISA, IHC

NB300-516
Western Blot: DNMT3B Antibody [NB300-516] - Homogenized mouse brain cells. Image from verified customer review.Immunohistochemistry-Paraffin: DNMT3B Antibody [NB300-516] - Analysis showing positive staining in the nucleus and cytoplasm of Human breast carcinoma (right) compared with a negative control in the absence of primary antibody (left).

Rabbit Polyclonal
Species Human, Mouse
Applications WB, Simple Western, ChIP

     1 Review

5 Publications
NB200-111
Western Blot: p14ARF/CDKN2A Antibody [NB200-111] - ARF expression levels in OCI/AML3 cells after proteasome treatment. This image was submitted via customer Review.Immunocytochemistry/Immunofluorescence: p14ARF/CDKN2A Antibody [NB200-111] - p14ARF antibody was tested in HeLa cells with Dylight 488 (green). Nuclei were counterstained with DAPI (blue). Tubulin was stained with alpha tubulin (red).

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ELISA, Flow

     2 Reviews

16 Publications
NB100-56519
Western Blot: DNMT1 Antibody (60B1220.1) [NB100-56519] - analysis of Dnmt1 in 2102EP (human embryonic carcinoma) in the A) absence and B) presence of immunizing peptide, recombinant human Dnmt1 protein in the C) absence and D) presence of immunizing peptide, NIH 3T3 (mouse embryonic fribroblast) in the E) absence and F) presence of immunizing peptide, and D3 (mouse embryonic stem cell) in the G) absence and H) presence of immunizing peptide using Dnmt1 antibody. this antibody. 5 ug/ml for testing the human cell line and 0.1 ug/ml was used for testing the recombinant protein and the mouse cell lines. ’s goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.Immunocytochemistry/Immunofluorescence: DNMT1 Antibody (60B1220.1) [NB100-56519] - Dnmt1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). An antibody dilution of 1:10 was used. Image objective 40x.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, ChIP

     2 Reviews

109 Publications
NB200-103
Western Blot: p53 Antibody (PAb 240) [NB200-103] - WB analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-p53 (PAb 240) [NB200-103] at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, Flow

     2 Reviews

22 Publications
NBP2-67381
Western Blot: MLH1 Antibody (SP08-04) [NBP2-67381] - Analysis of MLH1 on A431 cells lysates using anti-MLH1 antibody at 1/1,000 dilution.Immunocytochemistry/Immunofluorescence: MLH1 Antibody (SP08-04) [NBP2-67381] - Staining MLH1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

NB100-168
Western Blot: MGMT Antibody (MT 23.2) [NB100-168] - Analysis of MGMT (MT 23.2) antibody (NB100-168) - Detection of MGMT in MCF7 lysate using NB100-168 at 1:1000 dilution.Immunocytochemistry/Immunofluorescence: MGMT Antibody (MT 23.2) [NB100-168] - Detection of MGMT (Green) in Hela cells using NB100-168 at a 1:50 dilution. Nuclei (Blue) are counterstained using Hoechst 33258.

Mouse Monoclonal
Species Human, Mouse (Negative)
Applications WB, Simple Western, Flow

16 Publications
NBP2-03644
Western Blot: RASSF1 Antibody (2B11) [NBP2-03644] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RASSF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RASSF1.Immunocytochemistry/Immunofluorescence: RASSF1 Antibody (2B11) [NBP2-03644] Staining of COS7 cells transiently transfected by pCMV6-ENTRY RASSF1.

Mouse Monoclonal
Species Human
Applications WB, Flow, ICC/IF

1 Publication
NBP2-02450
Western Blot: Prostate Secretory Protein/PSP Antibody (6C7) [NBP2-02450] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Prostate Secretory Protein/PSP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Prostate Secretory Protein/PSP.Immunocytochemistry/Immunofluorescence: Prostate Secretory Protein/PSP Antibody (6C7) [NBP2-02450] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Prostate Secretory Protein/PSP.

Mouse Monoclonal
Species Human
Applications WB, ICC/IF, IHC

NBP1-92025
Western Blot: Invadolysin Antibody [NBP1-92025] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).Immunocytochemistry/Immunofluorescence: Invadolysin Antibody [NBP1-92025] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol & focal adhesion sites. Antibody staining is shown in green.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

352-MS/CF


Species Human

8 Publications
NBP2-15142
Western Blot: TMPRSS13 Antibody [NBP2-15142] - Sample (30 ug of whole cell lysate) A: PC-3 7.5% SDS PAGE; antibody diluted at 1:5000.Immunohistochemistry-Paraffin: TMPRSS13 Antibody [NBP2-15142] - Paraffin-embedded Breast carcinoma, using antibody at 1:250 dilution.

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

AF748
    Western  blot shows lysates of A431 human epithelial carcinoma cell line, A549 human  lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, P19  mouse embryonal carcinoma cell line, and 4T1 mouse breast cancer cell line.  PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse  E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody  (Catalog # <a class=    Simple  Western lane view shows lysates of 4T1 mouse breast cancer cell line, P19  mouse embryonal carcinoma cell line, A431 human epithelial carcinoma cell  line, and MCF‑7 human breast cancer cell line, loaded at  0.2 mg/mL. A specific band was detected for E‑Cadherin at  approximately 128 kDa (as indicated) using 5 µg/mL of Goat  Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal  Antibody (Catalog # AF748) followed by 1:50 dilution of HRP-conjugated  Anti-Goat IgG Secondary Antibody (Catalog # <a class=

Goat Polyclonal
Species Human, Mouse
Applications WB, Simple Western, Flow

     5 Reviews

23 Publications
292-G2
1 μg/lane of Recombinant Human IGF-II was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 7 kDa.Recombinant Human IGF-II (Catalog # 292‑G2) stimulates cell proliferation in the MCF-7 human breast cancer cell line. The ED<SUB>50</SUB> for this effect is 1.5‑6 ng/mL.


Species Human

     1 Review

29 Publications
NBP2-45603
Western Blot: p15INK4b/CDKN2B Antibody (3B6) [NBP2-45603] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN2B.Immunohistochemistry: p15INK4b/CDKN2B Antibody (3B6) [NBP2-45603] - Analysis of Adenocarcinoma of Human endometrium tissue.

Mouse monoclonal
Species Human
Applications WB, Flow, IHC

NBP1-85818
Western Blot: COMT Antibody [NBP1-85818] - Analysis in human cell lines MCF-7 and PC-3 using Anti-COMT antibody. Corresponding COMT RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.Immunohistochemistry-Paraffin: COMT Antibody [NBP1-85818] - Staining in human placenta and pancreas tissues using anti-COMT antibody. Corresponding COMT RNA-seq data are presented for the same tissues.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-P

1 Publication
NB100-91662
Western Blot: APC Antibody [NB100-91662] - Lane1:Hela cell lysate.  Lane2:HEK293T cell lysate.  Lane3:Rat testis tissue lysate.  Immunohistochemistry-Paraffin: APC Antibody [NB100-91662] - IHC-P analysis of a formalin fixed paraffin embedded tissue section of human colon cancer xenograft using 1: 50 dilution of APC antibody.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

NBP2-52487
Immunohistochemistry: H4/n Antibody (3E7D9) [NBP2-52487] - Analysis of paraffin-embedded esophageal cancer tissues using HIST2H4A(20Me) mouse mAb with DAB staining.Immunohistochemistry: H4/n Antibody (3E7D9) [NBP2-52487] - Analysis of paraffin-embedded colon cancer tissues using HIST2H4A(20Me) mouse mAb with DAB staining.

Mouse Monoclonal
Species Human
Applications WB, ELISA, Flow

NBP2-07996
Western Blot: HIST1H4F Overexpression Lysate (Adult Normal) [NBP2-07996] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIST1H4F.


Species Human

NBP1-82454
Western Blot: SUB1 Antibody [NBP1-82454] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.Immunocytochemistry/Immunofluorescence: SUB1 Antibody [NBP1-82454] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & nucleoli.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

2 Publications
NBP2-37607
Western Blot: MTHFR Antibody (5D3) [NBP2-37607] - Western blot analysis using MTHFR mouse mAb against Rat Heart cell lysate.Immunohistochemistry: MTHFR Antibody (5D3) [NBP2-37607] - Immunohistochemical analysis of paraffin-embedded lung cancer using MTHFR mouse mAb with DAB staining.

Mouse Monoclonal
Species Human, Rat
Applications WB, ELISA, IHC