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Insulin Secretion Pathway Bioinformatics

Insulin is a peptide hormone that is responsible for helping with the regulation of carbohydrate and fat metabolism, as it causes the cells of various tissues to absorb glucose in various forms from the bloodstream. Insulin is produced in the beta cells of the islets of Langerhans located in the pancreas, and is ready to be released into the body once it is cleaved from its signal peptide. Once glucose enters the body, it migrates to the pancreas an enters the beta cell, which leads to membrane depolarization and opens the calcium channels, allowing calcium to flow into the cell. The increase of calcium inside the cell initiates the secretion of insulin through secretory vesicles that fuse with the cell membrane, and insulin enters the bloodstream and begins its job of regulating blood and cell glucose levels. Deficiencies in insulin or resistance to the protein can lead to diseases including diabetes mellitus and polycystic ovary syndrome.

Insulin Secretion Bioinformatics Tool

Laverne is a handy bioinformatics tool to help facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Explore more on Insulin Secretion below! For more information on how to use Laverne, please read the How to Guide.
Vizit™, under license from BioVista Inc.

Top Research Reagents

We have 1730 products for the study of the Insulin Secretion Pathway that can be applied to Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

NBP1-47778
Western Blot: Glucokinase/GCK Antibody (3E3) [NBP1-47778] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GCK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GCK.Immunocytochemistry/Immunofluorescence: Glucokinase/GCK Antibody (3E3) [NBP1-47778] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GCK.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

NBP1-76626
Western Blot: C1qTNF1/CTRP1 Antibody [NBP1-76626] - Analysis of CTRP1 in human kidney cell lysate with CTRP1 (NT) antibody using NBP1-76626 at (A) 1 and (B) 2 ug/ml.Immunohistochemistry-Paraffin: C1qTNF1/CTRP1 Antibody [NBP1-76626] - Human kidney tissue with CTRP1 antibody at 10 ug/ml.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ELISA, ICC/IF

1 Publication
NBP1-97308
Immunohistochemistry: GLP-1R Antibody [NBP1-97308] - Analysis of GLP1R in mouse pancreas using DAB with hematoxylin counterstain.Knockdown Validated: GLP-1R Antibody [NBP1-97308] - Liraglutide reduces apoptosis of hCMSCs via PKA/i2-catenin pathway. The expression of GLP-1R and apoptotic proteins Bax, Bcl-2, cleaved caspase-9, and cleaved caspase-3 were detected by western blot with Si-GLP-1R and liraglutide. Image collected and cropped by Citeab from the following publication (Mesenchymal stem cells combined with liraglutide relieve acute lung injury through apoptotic signaling restrained by PKA/-catenin. Stem Cell Res Ther (2020) licensed under a CC-BY licence.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, DB

21 Publications
NBP2-12793
Immunohistochemistry-Paraffin: Insulin Receptor beta Antibody [NBP2-12793] - Staining shows expression of insulin receptor beta in the Cortex in mouse brain. Dilution 1:500. Image submitted by a verified customer review. Immunoprecipitation: Insulin Receptor beta Antibody [NBP2-12793] - Samples:  Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Insulin Receptor Beta antibody NBP2-12793 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate.  Insulin Receptor Beta was also immunoprecipitated by rabbit anti-Insulin Receptor Beta antibody, which recognizes an upstream epitope.  Detection: Chemiluminescence with exposure times of 30 seconds (A and B).

Rabbit Polyclonal
Species Human, Mouse
Applications WB, IHC, IHC-P

     1 Review

2 Publications
NBP2-33680
Immunohistochemistry-Paraffin: Amylin Antibody [NBP2-33680] - Staining of human placenta shows no positivity in trophoblastic cells as expected.Immunohistochemistry: Amylin Antibody [NBP2-33680] - Staining of human pancreas shows distinct cytoplasmic positivity in islets of Langerhans.

Rabbit Polyclonal
Species Human
Applications IHC, IHC-P

AF2419
Western blot shows lysates of  beta TC‑6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PDX‑1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2419) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <A class=NoLineLink href=PDX‑1/IPF1 was detected in immersion fixed BG01V human embryonic stem cells differentiated into pancreatic progenitor cells using Goat Anti-Human PDX‑1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # <A class=NoLineLink href=

Goat Polyclonal
Species Human
Applications WB, Simple Western, IHC

     3 Reviews

33 Publications
MAB1417
Insulin was detected in immersion fixed  beta TC‑6 mouse beta cell insulinoma cell line using Human/Mouse/Bovine Insulin Monoclonal Antibody (Catalog # MAB1417) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # <A class=NoLineLink href=

Rat Monoclonal
Species Human, Mouse, Bovine
Applications IHC, CyTOF-ready, ICC

14 Publications
AF1180
Western blot shows lysate of LoVo human colorectal adenocarcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <A class=NoLineLink href=Western blot shows lysate of human peripheral blood mononuclear cells (PBMC). PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <A class=NoLineLink href=

Goat Polyclonal
Species Human
Applications WB, Simple Western, IHC

     5 Reviews

28 Publications
MAB1249
Glucagon was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using 10 µg/mL Human/Mouse Glucagon Monoclonal Antibody (Catalog # MAB1249) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <A class=     beta TC‑6  mouse beta cell insulinoma cell line was stained with Mouse Anti-Human/Mouse  Glucagon Monoclonal Antibody (Catalog # MAB1249, filled histogram) or isotype  control antibody (Catalog # <a class=

Mouse Monoclonal
Species Human, Mouse
Applications CyTOF-ready, ICC, ICFlow

     3 Reviews

6 Publications
NBP2-37447
Western Blot: Somatostatin Antibody (7G5) [NBP2-37447] - Analysis using SST mAb against human SST recombinant protein. (Expected MW is 38.2 kDa)Immunohistochemistry-Paraffin: Somatostatin Antibody (7G5) [NBP2-37447] - Analysis of lung cancer tissues using SST mouse mAb with DAB staining.

Mouse Monoclonal
Species Human
Applications WB, ELISA, Flow

     1 Review

2 Publications
291-G1
1 μg/lane of Recombinant Human IGF-I/IGF-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 7 kDa.Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1) stimulates proliferation in the MCF-7 human breast cancer cell line. The ED<sub>50</sub> for this effect is 0.3‑1.5 ng/mL.


Species Human
Applications BA

     8 Reviews

172 Publications
MAB8864
GIP was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human GIP Monoclonal Antibody (Catalog # MAB8864) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

Mouse Monoclonal
Species Human
Applications IHC

     1 Review

1 Publication
NBP2-44318
Western Blot: G protein alpha Inhibitor 2 Antibody [NBP2-44318] - Analysis of human peripheral blood lymphocytes lysate (35 ug protein in RIPA buffer). Antibody at 2 ug/mL. Detected by chemiluminescence.Immunohistochemistry-Paraffin: G protein alpha Inhibitor 2 Antibody [NBP2-44318] - Staining of paraffin embedded Human Tonsil. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Goat Polyclonal
Species Human
Applications WB, IHC, IHC-P

1 Publication
NBP2-59320
Western Blot: SUR1 Antibody (S289-16) [NBP2-59320] - Analysis of mouse pancreatic islets. Lane 1: Molecular Weight Ladder. Lanes 2-5: Samples from pooled pancreatic islets. Load: ~10ug. Primary Antibody: Anti-SUR1 Monoclonal Antibody at 1:200 for ~16hrs at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1hr RT. Image from verified customer review.Immunocytochemistry/Immunofluorescence: SUR1 Antibody (S289-16) [NBP2-59320] - Glial fibrillary acidic protein (GFAP)-positive specimens from human contusion- traumatic brain injury (TBI) exhibit sulfonylurea receptor 1 (SUR1) expression in astrocytes.Double immunolabeling for SUR1 (red) and GFAP (green) showed astrocyte expression of SUR1; merged images confirm co-localization (yellow); in situ hybridization of the same tissue section for Abcc8 messenger RNA showed positive signal co-localized with GFAP-positive, SUR1-expressing astrocytes; arrowheads point to cells with all three signals. Image collected and cropped by CiteAb from the following publication (//www.liebertpub.com/doi/10.1089/neu.2018.5986) licensed under a CC-BY licence.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

     2 Reviews

3 Publications
NBP2-76944
Western Blot: Kir6.2 Antibody [NBP2-76944] - Western blot analysis of Kir6.2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: Human liver tissue lysateLane 2: Mouse liver tissue lysateImmunocytochemistry/Immunofluorescence: Kir6.2 Antibody [NBP2-76944] - ICC staining of Kir6.2 in LoVo cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluor488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

MOB00B
 Leptin/OB [HRP] Leptin/OB [HRP]


Species Mouse, Rat
Applications ELISA

     4 Reviews

150 Publications