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Autophagy Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Autophagy Pathway and Malignant Neoplasms, Neoplasms, Neurodegenerative Disorders, Nerve Degeneration, Inflammation. The study of the Autophagy Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Autophagy Pathway has been researched in relation to Cell Death, Pathogenesis, Macroautophagy, Programmed Cell Death, Autophagic Cell Death. The Autophagy Pathway complements our catalog of research reagents including antibodies and ELISA kits against BECN1, MTOR, MAP1LC3A, SQSTM1, ATG5.

Autophagy Bioinformatics Tool

Laverne is a handy bioinformatics tool to help facilitate scientific exploration of related genes, diseases and pathways based on co-citations. Explore more on Autophagy below! For more information on how to use Laverne, please read the How to Guide.
Vizit™, under license from BioVista Inc.

Top Research Reagents

We have 1742 products for the study of the Autophagy Pathway that can be applied to Chromatin Immunoprecipitation, Flow Cytometry, Western Blot, Immunocytochemistry/Immunofluorescence, Chromatin Immunoprecipitation (ChIP), Immunohistochemistry from our catalog of antibodies and ELISA kits.

NB500-249
Western Blot: Beclin 1 Antibody [NB500-249] - Expression of Beclin 1 mRNA and protein in cells and tissues. (A) Beclin 1 mRNA expression level up-regulated in gastric cancer cell lines compared with normal gastric epithelial cells by reverse transcription-PCR. (B) Beclin 1 protein expression level up-regulated in gastric cancer cell lines compared with normal gastric epithelial cells by western blotting. (C) Beclin 1 mRNA expression is elevated in primary gastric tumors compared with paired gastric adjacent noncancerous tissues by reverse transcription-PCR. (D) Beclin 1 protein expression is elevated in primary gastric tumors compared with paired gastric adjacent noncancerous tissues by western blotting. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0045968) licensed under a CC-BY licence.Simple Western: Beclin 1 Antibody [NB500-249] - Image shows a specific band for Beclin1 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, Flow

     8 Reviews

106 Publications
NB600-607
Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Rapamycin reduces mTOR phosphorylation induced by TII. Whole-cell lysates were prepared from astrocytes activated with TII for 2 h. 10 nM Rapamycin was added at the beginning of the experiment and equal amounts of protein were analyzed by western blot for phosphorylated mTOR kinase (p-mTOR) (upper gel) and consequently for beta-actin (lower gel). Image collected and cropped by CiteAb from the following publication (http://jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-8-1), licensed under a CC-BY licence.Immunohistochemistry-Paraffin: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Brown arrow= IBa1 positive cells, Green arrow= mTOR positive cells, Black arrow= mTOR and IBA1 positive cells. IHC-P Image submitted by a verified customer review.

Rabbit Polyclonal
Species Human, Rat
Applications WB, ELISA, IHC

     2 Reviews

9 Publications
NB100-2331
Immunohistochemistry: LC3A Antibody [NB100-2331] - Rapamycin increases autophagy in brains of PDAPP mice. Representative epifluorescent (c200x) image of hippocampal CA1 in control- and rapamycin-fed transgenic PDAPP mice stained with an anti-LC3 antibody. An increase in LC3-immunoreactive puncta was observed in CA1 projections of transgenic PDAPP mice following rapamycin administration. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0009979) licensed under a CC-BY licence.Western Blot: LC3A Antibody [NB100-2331] - Analysis of heart tissue lysates from mice which were subjected or not to 48 hours of starvation. The signal was developed using ECL method and this LC3 antibody was found to detect both forms of LC3, i.e. LC3-I and LC3-II. As expected, the levels of LC3-II form were higher in the heart tissue lysates from starved mice.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, ELISA

     20 Reviews

240 Publications
H00008878-M01
Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - WB analysis of recombinant p62 protein (immunogen, 74.51 KDa) using SQSTM1/p62 antibody (clone 2C11).Western Blot: p62/SQSTM1 Antibody (2C11) [H00008878-M01] - WB analysis of p62 in p62 WT and KO mouse hepatocytes and HepG2 cell lysate using anti-p62/SQSTM1 antibody clone 2C11. Sample lane1: p62 +/- mouse hepatocytes, lane 2: p62 -/- mouse hepatocytes, lane3: Wt mouse hepatocytes, lane 4: HepG2 cells.  This image is from a verified customer product review.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, ICC/IF

     8 Reviews

240 Publications
NB110-53818
Western Blot: ATG5 Antibody [NB110-53818] - Western blot analysis in mouse wildtype ES cell lysate (Lane 1) using NB110-53818. Lane 2 is a mouse ATG5 KO ES cell lysate (negative control). Atg5-/- ES cells from Dr. Noboru Mizushima [Mizushima, N. et al. J. Cell Biol. 152 (2001)] Photo courtesy of Dr. Beth Levine, UT Southwestern Medical Center.Western Blot: ATG5 Antibody [NB110-53818] - ERK phosphorylation in Atg5-/-cells depends on nutrient availability. Serum-deprived Atg5-/- MEFs display decreased ERK and p90RSK phosphorylation. Immunoblots for the indicated proteins in total lysates from 2 h serum-deprived WT and Atg5-/- MEFs. The bars represent mean+/-s.e.m. *P<0.05, ***P<0.001 compared with Con; Student's t-test, n=3. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/ncomms3799), licensed under a CC-BY licence.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, EM

     6 Reviews

197 Publications
H00002965-M01
Western Blot: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - GTF2H1 monoclonal antibody (M01), clone 1F12-1B5 Analysis of GTF2H1 expression in Jurkat.Immunocytochemistry/Immunofluorescence: GTF2H1 Antibody (1F12-1B5) [H00002965-M01] - Analysis of monoclonal antibody to GTF2H1 on HeLa cell. Antibody concentration 10 ug/ml.

Mouse Monoclonal
Species Human
Applications WB, ELISA, ICC/IF

2 Publications
NBP2-37399
Western Blot: dynactin 4 Antibody (3G9D7) [NBP2-37399] - Western blot analysis using DCTN4 mouse mAb against Raw264.7 (1) and NIH3T3 (2) cell lysate.Immunocytochemistry/Immunofluorescence: dynactin 4 Antibody (3G9D7) [NBP2-37399] - Immunofluorescence analysis of HepG2 cells using DCTN4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Mouse Monoclonal
Species Human
Applications WB, ELISA, Flow

NBP1-31381
Western Blot: NUP62 Antibody [NBP1-31381] - Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and blotted with Nucleoporin p62 antibody diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Immunocytochemistry/Immunofluorescence: Nucleoporin p62 Antibody [NBP1-31381] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nucleoporin p62 protein stained by Nucleoporin p62 antibody  diluted at 1:750. Blue: Hoechst 33342 staining. Scale bar = 10 um.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

1 Publication
NBP2-02627
Western Blot: IGF2BP2 Antibody (3F9) [NBP2-02627] Analysis of extracts (35ug) from 9 different cell lines by using anti-IGF2BP2 monoclonal antibody.Immunocytochemistry/Immunofluorescence: IGF2BP2 Antibody (3F9) [NBP2-02627] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY IGF2BP2.

Mouse Monoclonal
Species Human, Canine, Monkey
Applications WB, Flow, ICC/IF

NBP1-19151
Western Blot: SAM68 Antibody [NBP1-19151] - Whole cell lysate from HeLa cells. SAM68 was also immunoprecipitated by rabbit anti-SAM68 antibodies NBP1-19152 and NBP1-19153.Immunohistochemistry-Paraffin: SAM68 Antibody [NBP1-19151] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- SAM68 used at a dilution of 1:1,000 (0.2 ug/ml). Detection: DAB

Rabbit Polyclonal
Species Human, Bovine, Mammal
Applications WB, ICC/IF, IHC

     1 Review

2 Publications
AF887
Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Human PDGF (Catalog #<A class=NoLineLink href=Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and A549 human lung carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF‑I (Catalog # <A class=NoLineLink href=

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, IHC

23 Publications
NB100-56098
Western Blot: Bcl-2 Antibody [NB100-56098] - Analysis of Bcl-2 in whole cell lysate from Daoy cells. Cells were transfected with (1) scrambled control siRNA or (2) Bcl-2 siRNA. Image from verified customer review.Western Blot: Bcl-2 Antibody [NB100-56098] - Analysis of whole cell lysate (WCL) of Jurkat cells using anti-Bcl-2 antibody (NB100-56098) at 1:1000 dilution. HRP conjugated goat anti-rabbit IgG (H+L) cross adsorbed secondary antibody was used with ECL substrate for the detection of Bcl2 antibody bound to the blotted protein. This Bcl2 antibody detected a specific band at expected molecular weight marker position of Bcl2 protein (26kDa).

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-P

     2 Reviews

9 Publications
MAB6608
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ATG7 Monoclonal Antibody (Catalog # MAB6608) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <A class=NoLineLink href=ATG7 was detected in formalin fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human ATG7 Monoclonal Antibody (Catalog # MAB6608) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <A class=NoLineLink href=

Mouse Monoclonal
Species Human, Mouse
Applications WB, Simple Western, IHC

     1 Review

4 Publications
NB100-56708
Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Image of Caspase-3 Antibody (31A1067) - (Pro and Active).  Whole cell protein from Jurkat cells treated with and without 2 uM staurosporine as indicated was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST.  The membrane was probed with 5 ug/ml anti-Caspase 3 in 1% milk, and detected with an anti-mouse HRP secondary antibody using a Femto sensitivity chemiluminescence reagent.  Note the detection of both pro-caspase 3 at 35 kDa and the cleaved active caspase 3 at 15-17 kDa.Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Tissue section of human spleen using 1:200 dilution of Caspase-3 antibody (clone 31A1067). The staining was developed with HRP labeled anti-mouse IgG secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This Caspase 3 antibody generated primarily a specific cytoplasmic staining in a subset of spleenocytes with some nuclear signal in a few cells.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, EM

     1 Review

148 Publications
NBP2-82090
Western Blot: GATE-16/GABARAPL2 Antibody [NBP2-82090] - Analysis of GATE-16/GABARAPL2 in human brain tissue lysate with GATE-16/GABARAPL2 antibody at (A) 1 and (B) 2 ug/ml.Immunocytochemistry/Immunofluorescence: GATE-16/GABARAPL2 Antibody [NBP2-82090] - Immunofluorescence of GATE-16/GABARAPL2 in mouse brain tissue with GATE-16/GABARAPL2 Antibody at 20 ug/mL. Green: GATE-16/GABARAPL2 Antibody (NBP2-82090). Blue: DAPI staining

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ELISA, ICC/IF

NB200-103
Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-p53 (PAb 240) [NB200-103] at a 1:200 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, Flow

     3 Reviews

26 Publications
NBP1-55202
Western Blot: GABARAPL1 Antibody [NBP1-55202] - Hela Whole Cell lysates, Antibody Dilution: 1.0 ug/ml.Immunohistochemistry-Paraffin: GABARAPL1 Antibody [NBP1-55202] - Human Brain lysate tissue at an Antibody concentration of 5.0 ug/ml using anti-GABARAPL1 Antibody (ARP55398_P050).

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

NBP2-24738
Western Blot: ULK1 Antibody [NBP2-24738] - Analysis of ULK1 in Raji cell lysate in the 1) absence, 2) presence of immunizing peptide and 3) RAW using this antibody.Immunohistochemistry-Paraffin: ULK1 Antibody [NBP2-24738] - Analysis of human kidney using NBP2-24738

Rabbit Polyclonal
Species Human, Mouse, Monkey
Applications WB, ICC/IF, IHC

7 Publications
NBP2-67360
Western Blot: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of ERK2 on different lysates using anti-ERK2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: PC-12Immunocytochemistry/Immunofluorescence: ERK2 Antibody (SZ25-01) [NBP2-67360] - Staining ERK2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF