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![Western Blot: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis of 20ug of the indicated whole cell lysates onto a 4-12% Novex Bis-Tris polyacrylamide gel. Data courtesy of the Innovators Program.](http://images.novusbio.com/fullsize/PAX8-Antibody-1F8-3A8-Western-Blot-NBP2-29903-img0011.jpg "Western Blot: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis of 20ug of the indicated whole cell lysates onto a 4-12% Novex Bis-Tris polyacrylamide gel. Data courtesy of the Innovators Program.")
![Immunocytochemistry/Immunofluorescence: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis of PAX8 (green) in 293T (left panel) and COS7 cells (right panel). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a PAX8 monoclonal antibody at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight-554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/PAX8-Antibody-1F8-3A8-Immunocytochemistry-Immunofluorescence-NBP2-29903-img0005.jpg)
![Immunohistochemistry: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Staining in mouse thyroid tissue at pre-tumor stage (red). Dilution is 1:100. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/PAX8-Antibody-1F8-3A8-Immunocytochemistry-NBP2-29903-img0012.jpg)
![Western Blot: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis of 60ug of SKOV-3, COS7, and negative control HeLa whole cell lysates, and 10ul of PageRuler Plus Prestained Protein Ladder.](http://images.novusbio.com/fullsize/PAX8-Antibody-1F8-3A8-Western-Blot-NBP2-29903-img0009.jpg)
![Western Blot: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis of 25ug of HEK293T cell lysate overexpressing PAX8 (right lane) or empty vector control (left lane), and 10ul PageRuler Plus Prestained Protein Ladder.](http://images.novusbio.com/fullsize/PAX8-Antibody-1F8-3A8-Western-Blot-NBP2-29903-img0010.jpg)
![Immunohistochemistry-Paraffin: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis showing staining in the nucleus of mouse kidney tissue (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/PAX8-Antibody-1F8-3A8-Immunohistochemistry-Paraffin-NBP2-29903-img0006.jpg)
![Immunohistochemistry-Paraffin: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis of showing staining in the nucleus of human thyroid tissue (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/PAX8-Antibody-1F8-3A8-Immunohistochemistry-Paraffin-NBP2-29903-img0007.jpg)
![Immunohistochemistry-Paraffin: PAX8 Antibody (1F8-3A8) [NBP2-29903] - Analysis showing staining in the nucleus of human kidney tissue (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/PAX8-Antibody-1F8-3A8-Immunohistochemistry-Paraffin-NBP2-29903-img0008.jpg)
![Western Blot: PAX8 Antibody [NBP2-29907] - Analysis of HEK293 lysate overexpressing PAX8 (right lane) or empty vector control (left lane) and 10ul PageRuler Prestained Protein Ladder.](http://images.novusbio.com/fullsize/PAX8-Antibody-Western-Blot-NBP2-29907-img0011.jpg "Western Blot: PAX8 Antibody [NBP2-29907] - Analysis of HEK293 lysate overexpressing PAX8 (right lane) or empty vector control (left lane) and 10ul PageRuler Prestained Protein Ladder.")
![Immunocytochemistry/Immunofluorescence: PAX8 Antibody [NBP2-29907] - Analysis showing staining in the nucleus of human thyroid tissue (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/PAX8-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-29907-img0007.jpg)
![Western Blot: PAX8 Antibody [NBP2-29907] - Analysis of 75 ug of various whole cell lysates and 10ul of PageRuler Prestained Protein Ladder.](http://images.novusbio.com/fullsize/PAX8-Antibody-Western-Blot-NBP2-29907-img0009.jpg)
![Western Blot: PAX8 Antibody [NBP2-29907] - Analysis of 60ug of COS7 whole cell lysate (left lane), negative control HeLa lysate (right lane), and 10ul PageRuler Prestained Protein Ladder.](http://images.novusbio.com/fullsize/PAX8-Antibody-Western-Blot-NBP2-29907-img0010.jpg)
![Western Blot: PAX8 Antibody [NBP2-29907] - Analysis of 20ug of the indicated whole cell lysates onto a 4-12% Novex Bis-Tris polyacrylamide gel. Data courtesy of the Innovators Program.](http://images.novusbio.com/fullsize/PAX8-Antibody-Western-Blot-NBP2-29907-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: PAX8 Antibody [NBP2-29907] - Analysis of PAX8 (green) in 293T (left panel) and COS7 cells (right panel). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a PAX8 polyclonal antibody at a dilution of 1:400 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight-554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/PAX8-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-29907-img0006.jpg)
![Immunoprecipitation: PAX8 Antibody [NBP2-29907] - Analysis of PAX8 was performed using COS7 whole cell lysate. Antigen-antibody complexes were formed by incubating 500ug of lysate with 3ug of a PAX8 polyclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Samples was resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween-20 for 1 hour. The membrane was probed with a PAX8 polyclonal antibody at a dilution of 1:1000 overnight rotating at 4C. Membranes were washed in TBST, and probed with Clean-Blot IP Detection Reagent at a dilution of 1:1000 for at least one hour. Chemiluminescent detection was performed using SuperSignal West Pico.](http://images.novusbio.com/fullsize/PAX8-Antibody-Immunoprecipitation-NBP2-29907-img0008.jpg)