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![Western Blot: Profilin 4 Antibody [H00375189-B01P] - Analysis of PFN4 expression in transfected 293T cell line by PFN4 polyclonal antibody. Lane 1: PFN4 transfected lysate(14.19 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Profilin-4-Antibody-Western-Blot-H00375189-B01P-img0002.jpg "Western Blot: Profilin 4 Antibody [H00375189-B01P] - Analysis of PFN4 expression in transfected 293T cell line by PFN4 polyclonal antibody. Lane 1: PFN4 transfected lysate(14.19 KDa). Lane 2: Non-transfected lysate.")
![SDS-Page: Profilin 4 Protein [NBP1-41134] - Profilin-4, 16.4 kDa (149aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE](http://images.novusbio.com/fullsize/Profilin-4-Protein-SDS-Page-NBP1-41134-img0001.jpg "SDS-Page: Profilin 4 Protein [NBP1-41134] - Profilin-4, 16.4 kDa (149aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE")
![Immunocytochemistry/Immunofluorescence: Profilin 1 Antibody [NBP3-23344] - Immunocytochemical labeling of Profilin in aldehyde-fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with rabbit polyclonal anti-Profilin-1 (NBP3-23344) antibody. The antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23344_rabbit-profilin-1-pab-19920231856913.jpg "Immunocytochemistry/Immunofluorescence: Profilin 1 Antibody [NBP3-23344] - Immunocytochemical labeling of Profilin in aldehyde-fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with rabbit polyclonal anti-Profilin-1 (NBP3-23344) antibody. The antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.")
![Western Blot: Profilin 1 Antibody [NBP3-23344] - Western blot of HUVEC stimulated with Pervanadate (1 mM) for 30 min (lanes 1-4). The blots were treated with alkaline phosphatase to remove phosphorylation (lanes 2 & 4), then probed with anti-Profilin (a.a. 126-137) (lanes 1 & 2) or anti-Profilin (Tyr-129) phospho-specific (lanes 3 & 4). The antibodies detect profilin at 15 kDa. In lanes 3 & 4, the antibody also detects an unknown 20 kDa protein.](http://images.novusbio.com/fullsize/nbp3-23344_rabbit-profilin-1-pab-199202318202912.jpg)
![Immunocytochemistry/Immunofluorescence: Profilin 1 Antibody [NBP3-23341] - Immunocytochemical labeling of Profilin in aldehyde-fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with rabbit polyclonal anti-Profilin-1 (NBP3-23341) antibody. The antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23341_rabbit-profilin-1-pab-119202316331623.jpg "Immunocytochemistry/Immunofluorescence: Profilin 1 Antibody [NBP3-23341] - Immunocytochemical labeling of Profilin in aldehyde-fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with rabbit polyclonal anti-Profilin-1 (NBP3-23341) antibody. The antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.")
![Western Blot: Profilin 1 Antibody [NBP3-23341] - Western blot of Jurkat stimulated with calyculin A (100 nM) for 30 min (lanes 1-6). The blots were probed with anti-Profilin (a.a. 132-140) (lanes 1-3) or anti-Profilin (Ser-138) phospho-specific (lanes 4-6). Both antibodies were used in the absence (lanes 1 & 4) or presence of unphosphorylated Profilin (Ser-138) (lanes 2 & 5) or phospho-Profilin (Ser-138) (lanes 3 & 6) blocking peptides.](http://images.novusbio.com/fullsize/nbp3-23341_rabbit-profilin-1-pab-1992023199285.jpg)
![Western Blot: Profilin 1 [p Ser138] Antibody [NBP3-23342] - Western blot of Jurkat stimulated with calyculin A (100 nM) for 30 min (lanes 1-6). The blots were probed with anti-Profilin (a.a. 132-140) (lanes 1-3) or anti-Profilin (Ser-138) phospho-specific (lanes 4-6). Both antibodies were used in the absence (lanes 1 & 4) or presence of unphosphorylated Profilin (Ser-138) (lanes 2 & 5) or phospho-Profilin (Ser-138) (lanes 3 & 6) blocking peptides.](http://images.novusbio.com/fullsize/nbp3-23342_rabbit-profilin-1-p-ser138-pab-18920231735520.jpg "Western Blot: Profilin 1 [p Ser138] Antibody [NBP3-23342] - Western blot of Jurkat stimulated with calyculin A (100 nM) for 30 min (lanes 1-6). The blots were probed with anti-Profilin (a.a. 132-140) (lanes 1-3) or anti-Profilin (Ser-138) phospho-specific (lanes 4-6). Both antibodies were used in the absence (lanes 1 & 4) or presence of unphosphorylated Profilin (Ser-138) (lanes 2 & 5) or phospho-Profilin (Ser-138) (lanes 3 & 6) blocking peptides.")
![Western Blot: Profilin 1 [p Ser138] Antibody [NBP3-23342] - Western blot of human recombinant Profilin-1 phosphorylated in vitro with PKC alpha kinase then untreated (-) or treated with lambdaphosphatase (+). The blots were probed with anti-Profilin (a.a. 126-137) (left panel) or anti-Profilin (Ser-138) phospho-specific (right panel) antibodies at 1:1000.](http://images.novusbio.com/fullsize/nbp3-23342_rabbit-profilin-1-p-ser138-pab-199202312523637.jpg)
![Western Blot: Profilin 1 [p Tyr129] Antibody [NBP3-23345] - Western blot of HUVEC stimulated with Pervanadate (1 mM) for 30 min (lanes 1-4). The blots were treated with alkaline phosphatase to remove phosphorylation (lanes 2 & 4), then probed with anti-Profilin (a.a. 126-137) (lanes 1 & 2) or anti-Profilin (Tyr-129) phospho-specific (lanes 3 & 4). The antibodies detect profilin at 15 kDa. In lanes 3 & 4, the antibody also detects an unknown 20 kDa protein.](http://images.novusbio.com/fullsize/nbp3-23345_rabbit-profilin-1-p-tyr129-pab-19920231856966.jpg "Western Blot: Profilin 1 [p Tyr129] Antibody [NBP3-23345] - Western blot of HUVEC stimulated with Pervanadate (1 mM) for 30 min (lanes 1-4). The blots were treated with alkaline phosphatase to remove phosphorylation (lanes 2 & 4), then probed with anti-Profilin (a.a. 126-137) (lanes 1 & 2) or anti-Profilin (Tyr-129) phospho-specific (lanes 3 & 4). The antibodies detect profilin at 15 kDa. In lanes 3 & 4, the antibody also detects an unknown 20 kDa protein.")