| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34553-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47741-0.1mg | |||
| IHC-Prediluted | NBP2-48203 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52685-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-75903SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-75911SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB200-206-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52671-0.025mg | |||
![Western Blot: EGF R/ErbB1 [p Tyr992] Antibody (EM-12) [NBP1-44894] - Analysis of EGFR (phospho-Tyr992) by mouse monoclonal antibody EM-12 in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).](http://images.novusbio.com/fullsize/EGF-R-ErbB1-p-Tyr992-Antibody-EM-12-Western-Blot-NBP1-44894-img0001.jpg "Western Blot: EGF R/ErbB1 [p Tyr992] Antibody (EM-12) [NBP1-44894] - Analysis of EGFR (phospho-Tyr992) by mouse monoclonal antibody EM-12 in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).")
![Immunoprecipitation: EGF R/ErbB1 [p Tyr992] Antibody (EM-12) [NBP1-44894] - EGFR from EGF-treated A431 cells by monoclonal antibodies EM-12 (A), EM-13 (B), a commercial anti-EGFR polyclonal antibody (C) and anti-EGFR monoclonal (D). The precipitates were immunoblotted with EM-12 or EM-13 antibody, and goat anti-mouse-HRP.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-p-Tyr992-Antibody-EM-12-Immunoprecipitation-NBP1-44894-img0002.jpg)
![Immunoprecipitation: EGF R/ErbB1 [p Tyr992] Antibody (EM-12) [NBP1-44894] - EGFR from EGF-treated A431 cells by phosphospecific monoclonal antibodies EM-12 (A), EM-13 (B), a commercial anti-EGFR polyclonal antibody (C) and anti-EGFR monoclonal (D). The precipitates were immunoblotted with a commercial anti-EGFR polyclonal and goat anti-rabbit-HRP.](http://images.novusbio.com/fullsize/EGF-R-ErbB1-p-Tyr992-Antibody-EM-12-Immunoprecipitation-NBP1-44894-img0003.jpg)
![Immunohistochemistry-Paraffin: EGFR Antibody (SPM622) [NBP2-45149] - Human Placenta stained with EGFR Monoclonal Antibody (SPM622).](http://images.novusbio.com/fullsize/EGFR-Antibody-SPM622-Immunohistochemistry-Paraffin-NBP2-45149-img0001.jpg "Immunohistochemistry-Paraffin: EGFR Antibody (SPM622) [NBP2-45149] - Human Placenta stained with EGFR Monoclonal Antibody (SPM622).")
![Flow Cytometry: EGFR Antibody (Matuzumab) - Chimeric [NBP2-52685] - Flow-cytometry using the anti-EGFR antibody Matuzumab. HeLa (A) and A431 (B) cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of Matuzumab (NBP2-52685, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/EGFR-Antibody-Matuzumab-Chimeric-Flow-Cytometry-NBP2-52685-img0002.jpg "Flow Cytometry: EGFR Antibody (Matuzumab) - Chimeric [NBP2-52685] - Flow-cytometry using the anti-EGFR antibody Matuzumab. HeLa (A) and A431 (B) cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of Matuzumab (NBP2-52685, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.")
![Immunocytochemistry /Immunofluorescence: EGFR Antibody (Matuzumab) - Chimeric - Azide and BSA Free [NBP2-52685] - Immunofluorescence staining of fixed A431 cells with anti-EGFR antibody Matuzumab. Immunofluorescence analysis of unpermeabilisd paraformaldehyde fixed A431 cells on Shi-fix coverslips stained with NBP2-52685 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-52685, DAPI, merged channels and an isotype control. The isotype control was stained with unimmunised rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-52685_rabbit-monoclonal-egfr-antibody-matuzumab-chimeric-74202522141213.png)
![Immunohistochemistry: EGFR Antibody (C225 (Cetuximab)) [NBP2-75903] - Anti-EGFR staining of formaldehyde fixed paraffin embedded rat stomach tissue, at 40x magnification. The human IgG1-chimeric version of C225 (NBP2-75903) was used to stain samples at a concentration of 5 ug/ml.](http://images.novusbio.com/fullsize/EGFR-Antibody-C225-Cetuximab-Immunohistochemistry-NBP2-75903-img0002.jpg "Immunohistochemistry: EGFR Antibody (C225 (Cetuximab)) [NBP2-75903] - Anti-EGFR staining of formaldehyde fixed paraffin embedded rat stomach tissue, at 40x magnification. The human IgG1-chimeric version of C225 (NBP2-75903) was used to stain samples at a concentration of 5 ug/ml.")
![ELISA: EGFR Antibody (C225 (Cetuximab)) [NBP2-75903] - ELISA Plate coated with human EGFR-Fc at a concentration of 2.0 mg/ml. A 2-fold serial dilution from 100 to 0.1 ng/ml was performed using HRP labelled cetuximab human IgG1 (NBP2-75903).](http://images.novusbio.com/fullsize/EGFR-Antibody-C225-Cetuximab-ELISA-NBP2-75903-img0001.jpg)
![ELISA: EGFR Antibody (Matuzumab) [NBP2-75911] - ELISA Plate coated with human EGFR-Fc at a concentration of 5 ug/ml. A 3-fold serial dilution from 100 to 0.1 ng/ml was performed using NBP2-75911. For detection, a 1:4000 dilution of HRP labelled anti-human kappa light chain antibody was used.](http://images.novusbio.com/fullsize/EGFR-Antibody-Matuzumab-ELISA-NBP2-75911-img0003.jpg "ELISA: EGFR Antibody (Matuzumab) [NBP2-75911] - ELISA Plate coated with human EGFR-Fc at a concentration of 5 ug/ml. A 3-fold serial dilution from 100 to 0.1 ng/ml was performed using NBP2-75911. For detection, a 1:4000 dilution of HRP labelled anti-human kappa light chain antibody was used.")
![Western Blot: EGFR [p Tyr1045] Antibody (PSH03-28) [NBP3-32760] - Western blot analysis of EGFR on different lysates with Rabbit anti-EGFR antibody (NBP3-32760) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: A431 treated with 100ng/mL EGF for 30 minutes cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 134 kDa Observed band size: 170 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32760) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32760_rabbit-egfr-p-tyr1045-mab-psh03-28-288202412461681.jpg "Western Blot: EGFR [p Tyr1045] Antibody (PSH03-28) [NBP3-32760] - Western blot analysis of EGFR on different lysates with Rabbit anti-EGFR antibody (NBP3-32760) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: A431 treated with 100ng/mL EGF for 30 minutes cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 134 kDa Observed band size: 170 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32760) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: EGFR [p Tyr1045] Antibody (PSH03-28) [NBP3-32760] - Immunocytochemistry analysis of A431 treated with or without 100ng/mL EGF for 60 minutes cells labeling EGFR with Rabbit anti-EGFR antibody (NBP3-32760) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-EGFR antibody (NBP3-32760) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32760_rabbit-egfr-p-tyr1045-mab-psh03-28-29202410484836.jpg)
![Flow Cytometry: EGFR [p Tyr1045] Antibody (PSH03-28) [NBP3-32760] - Flow cytometric analysis of A431 treated with or without 100ng/mL EGF for 60 minutes cells labeling EGFR. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32760, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32760_rabbit-egfr-p-tyr1045-mab-psh03-28-29202416453235.jpg)
![Western Blot: EGFR [p Tyr992] Antibody [NBP3-13265] - Untreated (-) and treated (+) A431 whole cell extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr992) antibody (NBP3-13265) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EGFR-[p-Tyr992]-Antibody-Western-Blot-NBP3-13265-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: EGFR [p Tyr992] Antibody [NBP3-13265] - Untreated (-) and treated (+) A431 whole cell extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr992) antibody (NBP3-13265) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: EGFR [p Tyr992] Antibody [NBP3-13265] - EGFR (phospho Tyr992) antibody detects EGFR (phospho Tyr992) protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EGFR (phospho Tyr992) protein stained by EGFR (phospho Tyr992) antibody (NBP3-13265) diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/EGFR-[p-Tyr992]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13265-img0002.jpg)
![Western Blot: EGFR [p Tyr992] Antibody [NBP3-13228] - Untreated (-) and treated (+) A431 whole cell extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr992) antibody (NBP3-13228) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EGFR-[p-Tyr992]-Antibody-Western-Blot-NBP3-13228-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: EGFR [p Tyr992] Antibody [NBP3-13228] - Untreated (-) and treated (+) A431 whole cell extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr992) antibody (NBP3-13228) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: EGFR [p Tyr992] Antibody [NBP3-13228] - EGFR (phospho Tyr992) antibody detects EGFR (phospho Tyr992) protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EGFR (phospho Tyr992) protein stained by EGFR (phospho Tyr992) antibody (NBP3-13228) diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/EGFR-[p-Tyr992]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13228-img0002.jpg)
![Western Blot: EGFR [p Tyr1148] Antibody [NBP3-13263] - Untreated (-) and treated (+) A431 whole cell extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr1148) antibody (NBP3-13263) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EGFR-[p-Tyr1148]-Antibody-Western-Blot-NBP3-13263-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: EGFR [p Tyr1148] Antibody [NBP3-13263] - Untreated (-) and treated (+) A431 whole cell extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Tyr1148) antibody (NBP3-13263) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: EGFR [p Tyr1148] Antibody [NBP3-13263] - EGFR (phospho Tyr1148) antibody detects EGFR (phospho Tyr1148) protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EGFR (phospho Tyr1148) protein stained by EGFR (phospho Tyr1148) antibody (NBP3-13263) diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/EGFR-[p-Tyr1148]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13263-img0002.jpg)