We're moving to rndsystems.com. Come with us!
After August 17, 2026, Novus Biologicals products and services will no longer be available on this website; you will access all products and services on rndsystems.com. Create your R&D Systems online account today.
![Western Blot: Troponin I Type 3 (cardiac) [p Ser24, p Ser23] Antibody [NBP3-13325] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Troponin I Type 3 (cardiac) (phospho Ser23/Ser24) antibody (NBP3-13325) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Troponin-I-Type-3-cardiac-p-Ser24-p-Ser23-Antibody-Western-Blot-NBP3-13325-img0002.jpg "Western Blot: Troponin I Type 3 (cardiac) [p Ser24, p Ser23] Antibody [NBP3-13325] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Troponin I Type 3 (cardiac) (phospho Ser23/Ser24) antibody (NBP3-13325) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: Troponin I Type 3 (cardiac) [p Ser24, p Ser23] Antibody [NBP3-13325] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Troponin I Type 3 (cardiac) (phospho Ser23/Ser24) antibody (NBP3-13325) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Troponin-I-Type-3-cardiac-p-Ser24-p-Ser23-Antibody-Western-Blot-NBP3-13325-img0001.jpg)
![Western Blot: Troponin I Type 3 (cardiac) [p Ser43] Antibody [NBP2-29515] - Western blot of mouse heart lysate showing specific immunolabeling of the ~25k cTnI protein phosphorylated at Ser43 (control). Phosphospecificity is shown in the second lane. The blot is identical to the control except that the lysate was incubated in lambda-Ptase (1400 units for 30 min). The immunolabeling is greatly decreased by treatment with lambda-Ptase.](http://images.novusbio.com/fullsize/Troponin-I-Type-3-cardiac-p-Ser43-Antibody-Western-Blot-NBP2-29515-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Troponin I Type 3 (cardiac) [p Ser43] Antibody [NBP2-29515] - Western blot of mouse heart lysate showing specific immunolabeling of the ~25k cTnI protein phosphorylated at Ser43 (control). Phosphospecificity is shown in the second lane. The blot is identical to the control except that the lysate was incubated in lambda-Ptase (1400 units for 30 min). The immunolabeling is greatly decreased by treatment with lambda-Ptase.")
![Western Blot: Troponin I Type 3 (cardiac) [p Ser23, p Ser24] Antibody [NBP2-29525] - Western blot of mouse heart homogenate showing specific immunolabeling of the ~25k cTnI protein phosphorylated at Ser23/24 (control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide), but not by the corresponding dephospho-peptide (not shown).](http://images.novusbio.com/fullsize/Troponin-I-Type-3-cardiac-p-Ser23-p-Ser24-Antibody-Western-Blot-NBP2-29525-img0001.jpg "Western Blot: Troponin I Type 3 (cardiac) [p Ser23, p Ser24] Antibody [NBP2-29525] - Western blot of mouse heart homogenate showing specific immunolabeling of the ~25k cTnI protein phosphorylated at Ser23/24 (control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide), but not by the corresponding dephospho-peptide (not shown).")
![Western Blot: Troponin I Type 3 (cardiac) Overexpression Lysate (Adult Normal) [NBP2-11142] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Troponin I Type 3 (cardiac).](http://images.novusbio.com/fullsize/Troponin-I-Type-3-cardiac-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11142-img0001.jpg "Western Blot: Troponin I Type 3 (cardiac) Overexpression Lysate (Adult Normal) [NBP2-11142] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Troponin I Type 3 (cardiac).")