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![Western Blot: Poly-Ubiquitin Antibody - K27 Linkage [NBP3-05658] - Western blot analysis of extracts of various cell lines, using Poly-Ubiquitin Rabbit pAb (NBP3-05658) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Poly-Ubiquitin Antibody - K27 Linkage-Western Blot-NBP3-05658-img0002.jpg "Western Blot: Poly-Ubiquitin Antibody - K27 Linkage [NBP3-05658] - Western blot analysis of extracts of various cell lines, using Poly-Ubiquitin Rabbit pAb (NBP3-05658) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Dot Blot: Poly-Ubiquitin Antibody - K27 Linkage [NBP3-05658] - Dot-blot analysis of all sorts of peptides using Poly-Ubiquitin antibody (NBP3-05658) at 1:1000 dilution.](http://images.novusbio.com/fullsize/Poly-Ubiquitin-Antibody---K27-Linkage-Dot-Blot-NBP3-05658-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Ubiquitin Antibody [NBP2-58398] - Staining of human cell line MCF7 shows localization to nucleoli, cytosol & endoplasmic reticulum.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58398-img0001.jpg "Immunocytochemistry/Immunofluorescence: Ubiquitin Antibody [NBP2-58398] - Staining of human cell line MCF7 shows localization to nucleoli, cytosol & endoplasmic reticulum.")
![Western Blot: Ubiquitin Overexpression Lysate (Adult Normal) [NBL1-15568] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Ubiquitin.](http://images.novusbio.com/fullsize/Ubiquitin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15568-img0002.jpg "Western Blot: Ubiquitin Overexpression Lysate (Adult Normal) [NBL1-15568] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Ubiquitin.")
![Western Blot: Poly-Ubiquitin Antibody - K63 Linkage [NBP3-05682] - Analysis of extracts of various cell lines, using K63-linkage Specific Polyubiquitin antibody (A18164) at 1:500 dilution.C6 cells were treated by MG132(50 uM) at 37 c for 90 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Poly-Ubiquitin-Antibody---K63-Linkage-Western-Blot-NBP3-05682-img0002.jpg "Western Blot: Poly-Ubiquitin Antibody - K63 Linkage [NBP3-05682] - Analysis of extracts of various cell lines, using K63-linkage Specific Polyubiquitin antibody (A18164) at 1:500 dilution.C6 cells were treated by MG132(50 uM) at 37 c for 90 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Dot Blot: Poly-Ubiquitin Antibody - K63 Linkage [NBP3-05682] - Dot-blot analysis of all sorts of peptides using Poly-Ubiquitin antibody (NBP3-05682) at 1:1000 dilution.](http://images.novusbio.com/fullsize/Poly-Ubiquitin-Antibody---K63-Linkage-Dot-Blot-NBP3-05682-img0003.jpg)