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![Western Blot: RIPK2 Antibody (Nick-1) [NBP1-97631] - Detection of endogenous human RIP2 in different human cell lines by the MAb to RIP2 (Nick-1). Method: Total cell extracts (50ug) from Jurkat (lane1), THP-1 (lane 2) and HT1080 (lane 3) cell ljnes were run on a SDS-gel, and revealed with MAb to RIP2 (Nick-1) (1:1000 in 5% milk powder) for 1 hour at rooom temperature followed by incubation with HRP-coupled anti-rat secondary antibody (1:4000).](http://images.novusbio.com/fullsize/RIPK2-Antibody-Nick-1-Western-Blot-NBP1-97631-img0002.jpg "Western Blot: RIPK2 Antibody (Nick-1) [NBP1-97631] - Detection of endogenous human RIP2 in different human cell lines by the MAb to RIP2 (Nick-1). Method: Total cell extracts (50ug) from Jurkat (lane1), THP-1 (lane 2) and HT1080 (lane 3) cell ljnes were run on a SDS-gel, and revealed with MAb to RIP2 (Nick-1) (1:1000 in 5% milk powder) for 1 hour at rooom temperature followed by incubation with HRP-coupled anti-rat secondary antibody (1:4000).")