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![Western Blot: PKC alpha [p Thr638] Antibody (S09-7C7) [NBP3-19548] - Western blot detection of PKC alpha in K562, C6 cell lysates using NBP3-19548 (1:1000 diluted). Predicted band size: 77kDa. Observed band size: 80kDa.](http://images.novusbio.com/fullsize/PKC-alpha-p-Thr638-Antibody-S09-7C7-Western-Blot-NBP3-19548-img0002.jpg "Western Blot: PKC alpha [p Thr638] Antibody (S09-7C7) [NBP3-19548] - Western blot detection of PKC alpha in K562, C6 cell lysates using NBP3-19548 (1:1000 diluted). Predicted band size: 77kDa. Observed band size: 80kDa.")
![Immunohistochemistry-Paraffin: PKC alpha [p Thr638] Antibody (S09-7C7) [NBP3-19548] - Immunohistochemistry of PKC alpha in paraffin-embedded human colon cancer tissue using NBP3-19548 at dilution 1/100](http://images.novusbio.com/fullsize/PKC-alpha-p-Thr638-Antibody-S09-7C7-Immunohistochemistry-Paraffin-NBP3-19548-img0001.jpg)
![Western Blot: PKC alpha [p Thr 638] Antibody (SR2239) [NBP3-21584] - Western blot analysis using NBP3-21584 in (1) Jurkat cell lysate treated with TPA; (2) Jurkat cell lysate.](http://images.novusbio.com/fullsize/nbp3-21584_rabbit-pkc-alpha-p-thr-638-mab-sr2239-5720231754819.jpg "Western Blot: PKC alpha [p Thr 638] Antibody (SR2239) [NBP3-21584] - Western blot analysis using NBP3-21584 in (1) Jurkat cell lysate treated with TPA; (2) Jurkat cell lysate.")
![Immunohistochemistry-Paraffin: PKC alpha Antibody (133) [NBP3-07334] - Formalin-fixed, paraffin-embedded human tonsil stained with PKC alpha Mouse Monoclonal Antibody (133).](http://images.novusbio.com/fullsize/PKC-alpha-Antibody-133-Immunohistochemistry-Paraffin-NBP3-07334-img0001.jpg "Immunohistochemistry-Paraffin: PKC alpha Antibody (133) [NBP3-07334] - Formalin-fixed, paraffin-embedded human tonsil stained with PKC alpha Mouse Monoclonal Antibody (133).")
![Immunohistochemistry-Paraffin: PKC alpha Antibody (133) [NBP3-07334] - Formalin-fixed, paraffin-embedded human tonsil stained with PKC alpha Mouse Monoclonal Antibody (133).](http://images.novusbio.com/fullsize/nbp3-07334_mouse-monoclonal-pkc-alpha-antibody-133-17720241044642.jpg)
![Western Blot: PKC alpha [p Ser657] Antibody [NBP3-13311] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC alpha (phospho Ser657) antibody (NBP3-13311) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/PKC-alpha-[p-Ser657]-Antibody-Western-Blot-NBP3-13311-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: PKC alpha [p Ser657] Antibody [NBP3-13311] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC alpha (phospho Ser657) antibody (NBP3-13311) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Biological Strategies Validation. </b>Western Blot: PKC alpha [p Ser657] Antibody [NBP3-13311] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC alpha (phospho Ser657) antibody (NBP3-13311) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PKC-alpha-[p-Ser657]-Antibody-Western-Blot-NBP3-13311-img0001.jpg)
![Western Blot: PKC alpha [p Ser319] Antibody [NBP3-05438] - Western blot analysis of extracts of HeLa cells, using PKC alpha antibody (NBP3-05438) at 1:1000 dilution.HeLa cells were treated by PMA/TPA (200 nM) at 37C for 30 minutes after serum-starvation overnight.Hela cells were treated by ATP(5 mM) at 30C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/PKC alpha [p Ser319] Antibody-Western Blot-NBP3-05438-img0001.jpg "Western Blot: PKC alpha [p Ser319] Antibody [NBP3-05438] - Western blot analysis of extracts of HeLa cells, using PKC alpha antibody (NBP3-05438) at 1:1000 dilution.HeLa cells were treated by PMA/TPA (200 nM) at 37C for 30 minutes after serum-starvation overnight.Hela cells were treated by ATP(5 mM) at 30C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: PKC alpha [p Ser226] Antibody [NBP3-05437] - Western blot analysis of extracts of 293 cells, using PKC alpha antibody (NBP3-05437) at 1:1000 dilution. 293T cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.](http://images.novusbio.com/fullsize/PKC-alpha-[p-Ser226]-Antibody-Western-Blot-NBP3-05437-img0001.jpg "Western Blot: PKC alpha [p Ser226] Antibody [NBP3-05437] - Western blot analysis of extracts of 293 cells, using PKC alpha antibody (NBP3-05437) at 1:1000 dilution. 293T cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.")
![Western Blot: PKC Alpha, Beta, Gamma Antibody (9O5J1) [NBP3-33469] - Western blot analysis of various lysates, using PKC Alpha, Beta, Gamma Rabbit mAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilutionLysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.](http://images.novusbio.com/fullsize/nbp3-33469_rabbit-pkc-alpha-beta-gamma-mab-9o5j1-101220241243921.jpg "Western Blot: PKC Alpha, Beta, Gamma Antibody (9O5J1) [NBP3-33469] - Western blot analysis of various lysates, using PKC Alpha, Beta, Gamma Rabbit mAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilutionLysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.")
![Western Blot: PKC alpha [p Thr497] Antibody [NBP3-12925] - Untreated (-) and treated (+) Jurkat whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC alpha (phospho Thr497) antibody (NBP3-12925) diluted at 1:1000. (CIP: Calf-intestinal alkaline phosphatase)](http://images.novusbio.com/fullsize/PKC-alpha-[p-Thr497]-Antibody-Western-Blot-NBP3-12925-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: PKC alpha [p Thr497] Antibody [NBP3-12925] - Untreated (-) and treated (+) Jurkat whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC alpha (phospho Thr497) antibody (NBP3-12925) diluted at 1:1000. (CIP: Calf-intestinal alkaline phosphatase)")
![Western Blot: PKC alpha Overexpression Lysate (Adult Normal) [NBP2-04234] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PKC alpha.](http://images.novusbio.com/fullsize/PKC-alpha-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04234-img0001.jpg "Western Blot: PKC alpha Overexpression Lysate (Adult Normal) [NBP2-04234] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PKC alpha.")
![ELISA: Human PKC alpha - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39036] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39036_human-pkc-alpha-ready-to-use-elisa-kit-colorimetric-2110202415403134.png "ELISA: Human PKC alpha - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39036] - Standard Curve Reference")
![ELISA: Human PKC alpha ELISA Kit (Colorimetric) [NBP3-39035] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39035_human-pkc-alpha-elisa-kit-colorimetric-2110202415403175.png "ELISA: Human PKC alpha ELISA Kit (Colorimetric) [NBP3-39035] - Standard Curve Reference")