| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26437-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48247 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20940 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14033 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14033 | |||
![Immunohistochemistry: Pax6 Antibody (22C3) [NBP3-26437] - Image of Pax6 Antibody (22C3) diluted at 1:100 and staining in paraffin-embedded human pancreati tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.55% DAB.](http://images.novusbio.com/fullsize/nbp3-26437_rabbit-pax6-mab-22c3-262202414225221.jpg "Immunohistochemistry: Pax6 Antibody (22C3) [NBP3-26437] - Image of Pax6 Antibody (22C3) diluted at 1:100 and staining in paraffin-embedded human pancreati tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.55% DAB.")
![Immunohistochemistry: Pax6 Antibody (22C3) [NBP3-26437] - Image of Pax6 Antibody (22C3) diluted at 1:100 and staining in paraffin-embedded human pancreati tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.55% DAB.](http://images.novusbio.com/fullsize/nbp3-26437_rabbit-pax6-mab-22c3-262202415475614.jpg)
![Flow Cytometry: Pax6 Antibody (22C3) [NBP3-26437] - Overlay Peak curve showing SH-SY5Y cells stained with Pax6 Antibody (22C3) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26437_rabbit-pax6-mab-22c3-282202414312722.jpg)
![Western Blot: Pax6 Antibody (S01-1G8) [NBP3-19528] - Western blot detection of Pax6 in K562, Hela cell lysates using NBP3-19528 (1:1000 diluted). Predicted band size: 47kDa. Observed band size: 47kDa.](http://images.novusbio.com/fullsize/Pax6-Antibody-S01-1G8-Western-Blot-NBP3-19528-img0001.jpg "Western Blot: Pax6 Antibody (S01-1G8) [NBP3-19528] - Western blot detection of Pax6 in K562, Hela cell lysates using NBP3-19528 (1:1000 diluted). Predicted band size: 47kDa. Observed band size: 47kDa.")
![Western Blot: Pax6 Antibody (S03-8G4) [NBP3-19729] - Western blot detection of Pax6 in Rat Brain lysates using NBP3-19729 (1:1000 diluted). Predicted band size: 47kDa. Observed band size: 47kDa.](http://images.novusbio.com/fullsize/Pax6-Antibody-S03-8G4-Western-Blot-NBP3-19729-img0001.jpg "Western Blot: Pax6 Antibody (S03-8G4) [NBP3-19729] - Western blot detection of Pax6 in Rat Brain lysates using NBP3-19729 (1:1000 diluted). Predicted band size: 47kDa. Observed band size: 47kDa.")
![Western Blot: PAX6 Overexpression Lysate (Adult Normal) [NBP2-08189] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PAX6.](http://images.novusbio.com/fullsize/PAX6-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-08189-img0001.jpg "Western Blot: PAX6 Overexpression Lysate (Adult Normal) [NBP2-08189] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PAX6.")
![Immunoprecipitation:Pax6 AntibodyNBP3-29294] -Detection of human PAX6 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PAX6 antibody NBP3-29294 (lot NBP3-29294-1) used for IP at 6 µg per reaction. PAX6 was also immunoprecipitated by rabbit anti-PAX6 antibody. For blotting immunoprecipitated PAX6, NBP3-29294 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-29294_rabbit-pax6-pab-301220241518248.jpg "Immunoprecipitation:Pax6 AntibodyNBP3-29294] -Detection of human PAX6 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PAX6 antibody NBP3-29294 (lot NBP3-29294-1) used for IP at 6 µg per reaction. PAX6 was also immunoprecipitated by rabbit anti-PAX6 antibody. For blotting immunoprecipitated PAX6, NBP3-29294 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")