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![Western Blot: Tau Antibody [NBP3-11044] - Western blot analysis of Mouse, Rat Brain showing detection of Multiple bands due to different isoforms/aggregation states Tau protein using Rabbit Anti-Tau Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Mouse brain (15 ug). Lane 2: Rat brain (15 ug). Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking . Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: Multiple bands due to different isoforms/aggregation states. Other Band(s): 50 kDa, 65 kDa, 100 kDa.](http://images.novusbio.com/fullsize/Tau-Antibody-Western-Blot-NBP3-11044-img0001.jpg "Western Blot: Tau Antibody [NBP3-11044] - Western blot analysis of Mouse, Rat Brain showing detection of Multiple bands due to different isoforms/aggregation states Tau protein using Rabbit Anti-Tau Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Mouse brain (15 ug). Lane 2: Rat brain (15 ug). Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking . Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: Multiple bands due to different isoforms/aggregation states. Other Band(s): 50 kDa, 65 kDa, 100 kDa.")
![Dot Blot: Tau Antibody [NBP3-11044] - Dot blot analysis using Rabbit Anti-Tau Polyclonal Antibody. Tissue: Recombinant Tau Monomers and Fibrils. Species: Human. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking.](http://images.novusbio.com/fullsize/Tau-Antibody-Dot-Blot-NBP3-11044-img0002.jpg)
![ELISA: Rat Tau [p Ser610] ELISA Kit (Colorimetric) [NBP2-76716] - Standard Curve Reference](http://images.novusbio.com/fullsize/Rat-Tau-p-Ser610-ELISA-Kit-Colorimetric-ELISA-NBP2-76716-img0001.jpg "ELISA: Rat Tau [p Ser610] ELISA Kit (Colorimetric) [NBP2-76716] - Standard Curve Reference")
![ELISA: Rat Tau [p Ser610] ELISA Kit (Colorimetric) [NBP2-76716] - Samples were spiked with high concentrations of Rat Tau and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76716_rat-tau-p-ser610-elisa-kit-colorimetric-132202416235753.jpg)
![ELISA: Rat Tau [p Ser610] ELISA Kit (Colorimetric) [NBP2-76716] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76716_rat-tau-p-ser610-elisa-kit-colorimetric-432024903597.jpg)
![ELISA: Mouse Tau ELISA Kit (Colorimetric) [NBP2-81260] - Samples were spiked with high concentrations of Mouse Tau and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-81260_mouse-tau-elisa-kit-colorimetric-132202416232385.jpg "ELISA: Mouse Tau ELISA Kit (Colorimetric) [NBP2-81260] - Samples were spiked with high concentrations of Mouse Tau and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Tau ELISA Kit (Colorimetric) [NBP2-81260] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-81260_mouse-tau-elisa-kit-colorimetric-432024855058.jpg)
![Western Blot: Rabbit Tau [p Thr217] mAb (HL1276) - Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (p Thr217) antibody [HL1276] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-45156_rabbit-tau-p-thr217-mab-hl1276-azide-and-bsa-free-western-blot-2942025165210..jpg "Western Blot: Rabbit Tau [p Thr217] mAb (HL1276) - Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau (p Thr217) antibody [HL1276] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![ELISA: Rabbit Tau [p Thr217] mAb (HL1276) [NBP3-45156] - Indirect ELISA analysis was performed by coating the plate with Tau peptide and Tau (p Thr217) peptide (1000-15.63 ng/mL). Coated peptides were probed with Tau (p Thr217) antibody [HL1276] (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-45156_rabbit-tau-p-thr217-mab-hl1276-azide-and-bsa-free-elisa-2942025164816..jpg)
![Western Blot: Tau [p Ser202, p Ser199, p Ser198] Antibody [NBP3-16924] - Western blot analysis of extracts of NIH/3T3 cells, using Tau pAb (NBP3-16924) at 1:1000 dilution or Tau antibody.NIH/3T3 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 120s.](http://images.novusbio.com/fullsize/Tau-[p-Ser202-p-Ser199-p-Ser198]-Antibody-Western-Blot-NBP3-16924-img0001.jpg "Western Blot: Tau [p Ser202, p Ser199, p Ser198] Antibody [NBP3-16924] - Western blot analysis of extracts of NIH/3T3 cells, using Tau pAb (NBP3-16924) at 1:1000 dilution or Tau antibody.NIH/3T3 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 120s.")
![Western Blot: Tau [p Ser199] Antibody (JE66-80) [NBP3-32802] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32802) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse brain treated with pp for 1 hour tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 45-70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32802) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32802_rabbit-tau-p-ser199-mab-je66-80-288202412442962.jpg "Western Blot: Tau [p Ser199] Antibody (JE66-80) [NBP3-32802] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32802) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse brain treated with pp for 1 hour tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 45-70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32802) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Tau [p Ser214] Antibody (JE66-83) [NBP3-32805] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32805) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 79 kDa Observed band size: 40-55 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32805) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32805_rabbit-tau-p-ser214-mab-je66-83-288202412442910.jpg "Western Blot: Tau [p Ser214] Antibody (JE66-83) [NBP3-32805] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32805) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 79 kDa Observed band size: 40-55 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32805) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Tau [p Ser356] Antibody (JE46-86) [NBP3-32807] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32807) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate Lane 3: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 79 kDa Observed band size: 50-250 kDa Exposure time: 3 minutes 19 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32807) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32807_rabbit-tau-p-ser356-mab-je46-86-28820241246167.jpg "Western Blot: Tau [p Ser356] Antibody (JE46-86) [NBP3-32807] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32807) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate Lane 3: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 79 kDa Observed band size: 50-250 kDa Exposure time: 3 minutes 19 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32807) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Tau [p Ser202] Antibody (JE66-81) [NBP3-32804] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32804) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat hippocampus tissue lysate Lane 3: Mouse brain treated with pp for 1 hour tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 45-70 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32804) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32804_rabbit-tau-p-ser202-mab-je66-81-288202412442917.jpg "Western Blot: Tau [p Ser202] Antibody (JE66-81) [NBP3-32804] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32804) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat hippocampus tissue lysate Lane 3: Mouse brain treated with pp for 1 hour tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 45-70 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32804) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Tau [p Ser198] Antibody (JE66-79) [NBP3-32801] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32801) at 1/2,000 dilution. Lane 1: Mouse hippocampus tissue lysate Lane 2: Rat hippocampus tissue lysate Lane 3: Mouse hippocampus treated with pp for 1 hour tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 45-70 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32801) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32801_rabbit-tau-p-ser198-mab-je66-79-288202412442947.jpg "Western Blot: Tau [p Ser198] Antibody (JE66-79) [NBP3-32801] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32801) at 1/2,000 dilution. Lane 1: Mouse hippocampus tissue lysate Lane 2: Rat hippocampus tissue lysate Lane 3: Mouse hippocampus treated with pp for 1 hour tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 45-70 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32801) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Tau [p Ser199] Antibody (JE46-95) [NBP3-32803] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32803) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Human brain tissue lysate, the membrane treated with pp for 1 hour Lane 5: Mouse brain tissue lysate, the membrane treated with pp for 1 hour Lane 6: Rat brain tissue lysate, the membrane treated with pp for 1 hour Lysates/proteins at 20 ug/Lane. Predicted band size: 79 kDa Observed band size: 35-70 kDa Exposure time: 3 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32803) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32803_rabbit-tau-p-ser199-mab-je46-95-288202412461637.jpg "Western Blot: Tau [p Ser199] Antibody (JE46-95) [NBP3-32803] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32803) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Human brain tissue lysate, the membrane treated with pp for 1 hour Lane 5: Mouse brain tissue lysate, the membrane treated with pp for 1 hour Lane 6: Rat brain tissue lysate, the membrane treated with pp for 1 hour Lysates/proteins at 20 ug/Lane. Predicted band size: 79 kDa Observed band size: 35-70 kDa Exposure time: 3 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32803) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")