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![ELISA: Rat IL-21 ELISA Kit (Colorimetric) [NBP3-06780] - Samples were spiked with high concentrations of Rat IL-21 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-06780_rat-il-21-elisa-kit-colorimetric-132202416133691.jpg "ELISA: Rat IL-21 ELISA Kit (Colorimetric) [NBP3-06780] - Samples were spiked with high concentrations of Rat IL-21 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat IL-21 ELISA Kit (Colorimetric) [NBP3-06780] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-06780_rat-il-21-elisa-kit-colorimetric-4320248574372.jpg)
![ELISA: Mouse IL-21 ELISA Kit (Colorimetric) [NBP3-06779] - Samples were spiked with high concentrations of Mouse IL-21 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-06779_mouse-il-21-elisa-kit-colorimetric-132202416243480.jpg "ELISA: Mouse IL-21 ELISA Kit (Colorimetric) [NBP3-06779] - Samples were spiked with high concentrations of Mouse IL-21 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse IL-21 ELISA Kit (Colorimetric) [NBP3-06779] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-06779_mouse-il-21-elisa-kit-colorimetric-43202495078.jpg)
![Western Blot: IL21 Overexpression Lysate (Adult Normal) [NBP2-06520] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IL21.](http://images.novusbio.com/fullsize/IL21-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-06520-img0001.jpg "Western Blot: IL21 Overexpression Lysate (Adult Normal) [NBP2-06520] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IL21.")
![ELISA: IL-21 Antibody [NBP3-11321] - Recombinant Rabbit IL-21 detected using Chicken anti Rabbit IL-21 (AHP2826) as the capture reagent and Chicken anti Rabbit IL-21:Biotin as the detection reagent followed by Streptavidin:HRP.](http://images.novusbio.com/fullsize/IL-21-Antibody-ELISA-NBP3-11321-img0001.jpg "ELISA: IL-21 Antibody [NBP3-11321] - Recombinant Rabbit IL-21 detected using Chicken anti Rabbit IL-21 (AHP2826) as the capture reagent and Chicken anti Rabbit IL-21:Biotin as the detection reagent followed by Streptavidin:HRP.")
![Neutralization: IL-21 Antibody (MT216G/21.3m) [NBP3-18290] - The neutralizing capacity and ND50 was determined using ELISA andELISpot. A) HEK cells were transfected to express the IL-21 activation pathway with alkaline phosphatase as a reporter. Cells were stimulated in the presence of recombinant human IL-21 (10 ng/ml) with or without serial dilutions of NBP3-18290. After overnight incubation, enzyme activity was measured in ELISA. B) Human peripheral blood mononuclear cells (PBMC) were incubated with recombinant IL-21 (10 ng/ml) with or without serial dilutions of NBP3-18290. After 4 days the IgG-secreting B cells were quantified by ELISpot.](http://images.novusbio.com/fullsize/IL-21-Antibody-MT216G-21-3m-Neutralization-NBP3-18290-img0001.jpg "Neutralization: IL-21 Antibody (MT216G/21.3m) [NBP3-18290] - The neutralizing capacity and ND50 was determined using ELISA andELISpot. A) HEK cells were transfected to express the IL-21 activation pathway with alkaline phosphatase as a reporter. Cells were stimulated in the presence of recombinant human IL-21 (10 ng/ml) with or without serial dilutions of NBP3-18290. After overnight incubation, enzyme activity was measured in ELISA. B) Human peripheral blood mononuclear cells (PBMC) were incubated with recombinant IL-21 (10 ng/ml) with or without serial dilutions of NBP3-18290. After 4 days the IgG-secreting B cells were quantified by ELISpot.")
![ELISA: IL-21 ELISA Development Kit [NBP3-11754] - Standard curve reference](http://images.novusbio.com/fullsize/IL-21-ELISA-Development-Kit-ELISA-NBP3-11754-img0001.jpg "ELISA: IL-21 ELISA Development Kit [NBP3-11754] - Standard curve reference")
![Western Blot: IL-21 Antibody - BSA Free [NBP1-76740] - Validation in Human HL-60 Cell Lysate. Loading: 15 ug of lysates per lane.Antibodies: IL-21 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.(A) Absence of blocking peptide(B) Presence of blocking peptide.](http://images.novusbio.com/fullsize/nbp1-76740pep_il-21-antibody-blocking-peptide-2711202419251583.jpg "Western Blot: IL-21 Antibody - BSA Free [NBP1-76740] - Validation in Human HL-60 Cell Lysate. Loading: 15 ug of lysates per lane.Antibodies: IL-21 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.(A) Absence of blocking peptide(B) Presence of blocking peptide.")
![ELISA: IL-21 ELISA Kit [NBP1-84816] - Standard curve](http://images.novusbio.com/fullsize/IL-21-ELISA-Kit-ELISA-NBP1-84816-img0001.jpg "ELISA: IL-21 ELISA Kit [NBP1-84816] - Standard curve")