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![Western Blot: IL1 beta Overexpression Lysate (Adult Normal) [NBP2-04165] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IL1 beta.](http://images.novusbio.com/fullsize/IL1-beta-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04165-img0001.jpg "Western Blot: IL1 beta Overexpression Lysate (Adult Normal) [NBP2-04165] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IL1 beta.")
![ELISA: IL-1 beta/IL-1F2 Antibody [NBP3-11364] - Recombinant Rabbit IL-1 beta/IL-1F2 detected using Chicken anti Rabbit IL-1 beta/IL-1F2 as the capture reagent and Chicken anti Rabbit IL-1 beta/IL-1F2 beta:Biotin as the detection reagent followed by Streptavidin:HRP.](http://images.novusbio.com/fullsize/IL-1-beta-IL-1F2-Antibody-ELISA-NBP3-11364-img0001.jpg "ELISA: IL-1 beta/IL-1F2 Antibody [NBP3-11364] - Recombinant Rabbit IL-1 beta/IL-1F2 detected using Chicken anti Rabbit IL-1 beta/IL-1F2 as the capture reagent and Chicken anti Rabbit IL-1 beta/IL-1F2 beta:Biotin as the detection reagent followed by Streptavidin:HRP.")
![Western Blot: IL-1 beta/IL-1F2 Antibody (PS00-82) [NBP3-32450] - Western blot analysis of IL-1 beta/IL-1F2 on different lysates with Rabbit anti-IL-1 beta/IL-1F2 antibody (NBP3-32450) at 1/1,000 dilution. Lane 1: THP-1 whole cell lysate Lane 2: THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours and 300ng/mL BFA for 3 hours whole cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 31 kDa Observed band size: 31/28 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32450) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32450_rabbit-il-1-beta-il-1f2-mab-ps00-82-27820241521248.jpg "Western Blot: IL-1 beta/IL-1F2 Antibody (PS00-82) [NBP3-32450] - Western blot analysis of IL-1 beta/IL-1F2 on different lysates with Rabbit anti-IL-1 beta/IL-1F2 antibody (NBP3-32450) at 1/1,000 dilution. Lane 1: THP-1 whole cell lysate Lane 2: THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours and 300ng/mL BFA for 3 hours whole cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 31 kDa Observed band size: 31/28 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32450) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: IL-1 beta/IL-1F2 Antibody (PS01-39) [NBP3-32449] - Western blot analysis of IL-1 beta/IL-1F2 on different lysates with Rabbit anti-IL-1 beta/IL-1F2 antibody (NBP3-32449) at 1/1,000 dilution. Lane 1: THP-1 whole cell lysate Lane 2: THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours and 300ng/mL BFA for 3 hours whole cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 31 kDa Observed band size: 31/28 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32449) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32449_rabbit-il-1-beta-il-1f2-mab-ps01-39-278202415212445.jpg "Western Blot: IL-1 beta/IL-1F2 Antibody (PS01-39) [NBP3-32449] - Western blot analysis of IL-1 beta/IL-1F2 on different lysates with Rabbit anti-IL-1 beta/IL-1F2 antibody (NBP3-32449) at 1/1,000 dilution. Lane 1: THP-1 whole cell lysate Lane 2: THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours and 300ng/mL BFA for 3 hours whole cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 31 kDa Observed band size: 31/28 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32449) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")