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![Western Blot: HSP27 [p Ser82] Antibody [NBP3-13297] - Untreated (-) and treated (+) C2C12 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody (NBP3-13297) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/HSP27-[p-Ser82]-Antibody-Western-Blot-NBP3-13297-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: HSP27 [p Ser82] Antibody [NBP3-13297] - Untreated (-) and treated (+) C2C12 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody (NBP3-13297) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Biological Strategies Validation. </b>Western Blot: HSP27 [p Ser82] Antibody [NBP3-13297] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody (NBP3-13297) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HSP27-[p-Ser82]-Antibody-Western-Blot-NBP3-13297-img0001.jpg)
![Western Blot: HSP27 [p Ser82] Antibody [NBP3-13297] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody (NBP3-13297) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/HSP27-[p-Ser82]-Antibody-Western-Blot-NBP3-13297-img0002.jpg)
![Western Blot: HSP27 Antibody (S03-9F4) [NBP3-20041] - Western blot detection of HSP27 in Hela cell lysates using NBP3-20041 (1:1000 diluted). Predicted band size: 23kDa. Observed band size: 27kDa.](http://images.novusbio.com/fullsize/HSP27-Antibody-S03-9F4-Western-Blot-NBP3-20041-img0002.jpg "Western Blot: HSP27 Antibody (S03-9F4) [NBP3-20041] - Western blot detection of HSP27 in Hela cell lysates using NBP3-20041 (1:1000 diluted). Predicted band size: 23kDa. Observed band size: 27kDa.")
![Immunohistochemistry-Paraffin: HSP27 Antibody (S03-9F4) [NBP3-20041] - Immunohistochemical of HSP27 in Human breast cancer tissue using HNBP3-20041 at dilution 1/50](http://images.novusbio.com/fullsize/HSP27-Antibody-S03-9F4-Immunohistochemistry-Paraffin-NBP3-20041-img0001.jpg)
![SDS-Page: Recombinant Human HSP27 Protein [NBC1-18364] - HSP27, 22.7kDa (205aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE](http://images.novusbio.com/fullsize/Recombinant-Human-HSP27-Protein-SDS-Page-NBC1-18364-img0002.jpg "SDS-Page: Recombinant Human HSP27 Protein [NBC1-18364] - HSP27, 22.7kDa (205aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE")
![ELISA: Mouse HSP27 ELISA Kit (Colorimetric) [NBP2-76438] - Samples were spiked with high concentrations of Mouse HSP27 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76438_mouse-hsp27-elisa-kit-colorimetric-132202416204061.jpg "ELISA: Mouse HSP27 ELISA Kit (Colorimetric) [NBP2-76438] - Samples were spiked with high concentrations of Mouse HSP27 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse HSP27 ELISA Kit (Colorimetric) [NBP2-76438] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76438_mouse-hsp27-elisa-kit-colorimetric-432024963918.jpg)
![ELISA: Rat HSP27 ELISA Kit (Colorimetric) [NBP2-76439] - Samples were spiked with high concentrations of Rat HSP27 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76439_rat-hsp27-elisa-kit-colorimetric-1322024162040.jpg "ELISA: Rat HSP27 ELISA Kit (Colorimetric) [NBP2-76439] - Samples were spiked with high concentrations of Rat HSP27 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat HSP27 ELISA Kit (Colorimetric) [NBP2-76439] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76439_rat-hsp27-elisa-kit-colorimetric-432024983599.jpg)
![Western Blot: Hsp27 Overexpression Lysate (Adult Normal) [NBL1-11757] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Hsp27.](http://images.novusbio.com/fullsize/Hsp27-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11757-img0002.jpg "Western Blot: Hsp27 Overexpression Lysate (Adult Normal) [NBL1-11757] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Hsp27.")
![Western Blot: Hsp27 Antibody (JBW502) [NBP2-29821] - Untreated (lane 1) and heat shock and arsenite treated (lane 2) MCF-7 cell lysate was probed with 0.05ug/ml of anti-phospho-HSP27 (Ser 78). Arrow indicates phosphorylated HSP27.](http://images.novusbio.com/fullsize/Hsp27-Antibody-JBW502-Western-Blot-NBP2-29821-img0001.jpg "Western Blot: Hsp27 Antibody (JBW502) [NBP2-29821] - Untreated (lane 1) and heat shock and arsenite treated (lane 2) MCF-7 cell lysate was probed with 0.05ug/ml of anti-phospho-HSP27 (Ser 78). Arrow indicates phosphorylated HSP27.")
![ELISA: Human HSP27 ELISA Kit (Colorimetric) [KA0981] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.](http://images.novusbio.com/fullsize/Human-HSP27-ELISA-Kit-Colorimetric-ELISA-KA0981-img0001.jpg "ELISA: Human HSP27 ELISA Kit (Colorimetric) [KA0981] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.")
![ELISA: Human HSP27 [p Ser78] ELISA Kit (Colorimetric) [NBP2-62152] - Standard Reference Curve](http://images.novusbio.com/fullsize/Human-HSP27-p-Ser78-ELISA-Kit-Colorimetric-ELISA-NBP2-62152-img0001.jpg "ELISA: Human HSP27 [p Ser78] ELISA Kit (Colorimetric) [NBP2-62152] - Standard Reference Curve")
![ELISA: Human HSP27 [p Ser15] ELISA Kit (Colorimetric) [NBP2-62151] - A typical standard curve for HSP27 [p Ser15] ELISA kit. These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.](http://images.novusbio.com/fullsize/Human-HSP27-p-Ser15-ELISA-Kit-Colorimetric-ELISA-NBP2-62151-img0001.jpg "ELISA: Human HSP27 [p Ser15] ELISA Kit (Colorimetric) [NBP2-62151] - A typical standard curve for HSP27 [p Ser15] ELISA kit. These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.")