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![Western Blot: HSP90 Antibody (10D6) [NBP3-15144] - Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, HL-60 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, Jurkat whole cell lysate, PC3 whole cell lysate, Rat brain tissue. All lanes: HSP90 antibody at 1.25ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 90 KDa. Observed band size: 90 KDa.](http://images.novusbio.com/fullsize/HSP90-Antibody-10D6-Western-Blot-NBP3-15144-img0003.jpg "Western Blot: HSP90 Antibody (10D6) [NBP3-15144] - Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, HL-60 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, Jurkat whole cell lysate, PC3 whole cell lysate, Rat brain tissue. All lanes: HSP90 antibody at 1.25ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 90 KDa. Observed band size: 90 KDa.")
![Flow Cytometry: HSP90 Antibody (10D6) [NBP3-15144] - Overlay histogram showing Jurkat cells stained with HSP90 antibody (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/HSP90-Antibody-10D6-Flow-Cytometry-NBP3-15144-img0001.jpg)
![Immunoprecipitation: HSP90 Antibody (10D6) [NBP3-15144] - Immunoprecipitating HSP90 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of HSP90 antibody in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: HSP90 antibody (3ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (20ug).](http://images.novusbio.com/fullsize/HSP90-Antibody-10D6-Immunoprecipitation-NBP3-15144-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: HSP90 Antibody (4F8) [NBP3-15146] - Immunofluorescence staining of Hela cells with at HSP90 antibody 1:29, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/HSP90-Antibody-4F8-Immunocytochemistry-Immunofluorescence-NBP3-15146-img0001.jpg "Immunocytochemistry/Immunofluorescence: HSP90 Antibody (4F8) [NBP3-15146] - Immunofluorescence staining of Hela cells with at HSP90 antibody 1:29, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).")
![Immunohistochemistry: HSP90 Antibody (4F8) [NBP3-15146] - IHC image of HSP90 diluted at 1:87.5 and staining in paraffin-embedded human endometrial cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/HSP90-Antibody-4F8-Immunohistochemistry-NBP3-15146-img0002.jpg)
![Immunohistochemistry: HSP90 Antibody (4F8) [NBP3-15146] - IHC image of HSP90 antibody diluted at 1:87.5 and staining in paraffin-embedded human testis tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/HSP90-Antibody-4F8-Immunohistochemistry-NBP3-15146-img0003.jpg)
![ELISA: Mouse HSP90 ELISA Kit (Colorimetric) [NBP2-76449] - Samples were spiked with high concentrations of Mouse HSP90 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76449_mouse-hsp90-elisa-kit-colorimetric-1322024160121.jpg "ELISA: Mouse HSP90 ELISA Kit (Colorimetric) [NBP2-76449] - Samples were spiked with high concentrations of Mouse HSP90 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse HSP90 ELISA Kit (Colorimetric) [NBP2-76449] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76449_mouse-hsp90-elisa-kit-colorimetric-4320249163364.jpg)
![Western Blot: Hsp90A/B Antibody (5D7Y6) [NBP3-16532] - Western blot analysis of extracts of various cell lines, using Hsp90A/B Rabbit mAb (NBP3-16532) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Hsp90A-B-Antibody-5D7Y6-Western-Blot-NBP3-16532-img0001.jpg "Western Blot: Hsp90A/B Antibody (5D7Y6) [NBP3-16532] - Western blot analysis of extracts of various cell lines, using Hsp90A/B Rabbit mAb (NBP3-16532) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: HSP90 alpha Antibody (S06-9C1) [NBP3-19514] - Western blot detection of HSP90 alpha in K562, C6, Hela cell lysates using NBP3-19514 (1:1000 diluted). Predicted band size: 85kDa. Observed band size: 90kDa.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-S06-9C1-Western-Blot-NBP3-19514-img0001.jpg "Western Blot: HSP90 alpha Antibody (S06-9C1) [NBP3-19514] - Western blot detection of HSP90 alpha in K562, C6, Hela cell lysates using NBP3-19514 (1:1000 diluted). Predicted band size: 85kDa. Observed band size: 90kDa.")
![ELISA: Human HSP90 ELISA Kit (Colorimetric) [NBP2-76448] - Samples were spiked with high concentrations of Human HSP90 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76448_human-hsp90-elisa-kit-colorimetric-132202416225277.jpg "ELISA: Human HSP90 ELISA Kit (Colorimetric) [NBP2-76448] - Samples were spiked with high concentrations of Human HSP90 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human HSP90 ELISA Kit (Colorimetric) [NBP2-76448] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76448_human-hsp90-elisa-kit-colorimetric-432024857470.jpg)
![ELISA: Rat HSP90 ELISA Kit (Colorimetric) [NBP2-76450] - Samples were spiked with high concentrations of Rat HSP90 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76450_rat-hsp90-elisa-kit-colorimetric-132202416184852.jpg "ELISA: Rat HSP90 ELISA Kit (Colorimetric) [NBP2-76450] - Samples were spiked with high concentrations of Rat HSP90 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat HSP90 ELISA Kit (Colorimetric) [NBP2-76450] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76450_rat-hsp90-elisa-kit-colorimetric-4320249173423.jpg)
![ELISA: Human HSP90 ELISA Kit (Chemiluminescence) [NBP2-76643] - Samples were spiked with high concentrations of Human HSP90 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76643_human-hsp90-elisa-kit-chemiluminescence-132202416143080.jpg "ELISA: Human HSP90 ELISA Kit (Chemiluminescence) [NBP2-76643] - Samples were spiked with high concentrations of Human HSP90 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human HSP90 ELISA Kit (Chemiluminescence) [NBP2-76643] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76643_human-hsp90-elisa-kit-chemiluminescence-432024973646.jpg)
![ELISA: Mouse HSP90 ELISA Kit (Chemiluminescence) [NBP2-76644] - Samples were spiked with high concentrations of Mouse HSP90 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76644_mouse-hsp90-elisa-kit-chemiluminescence-13220241685340.jpg "ELISA: Mouse HSP90 ELISA Kit (Chemiluminescence) [NBP2-76644] - Samples were spiked with high concentrations of Mouse HSP90 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse HSP90 ELISA Kit (Chemiluminescence) [NBP2-76644] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76644_mouse-hsp90-elisa-kit-chemiluminescence-4320249115674.jpg)
![ELISA: Rat HSP90 ELISA Kit (Chemiluminescence) [NBP2-76645] - Samples were spiked with high concentrations of Rat HSP90 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-76645_rat-hsp90-elisa-kit-chemiluminescence-132202416173467.jpg "ELISA: Rat HSP90 ELISA Kit (Chemiluminescence) [NBP2-76645] - Samples were spiked with high concentrations of Rat HSP90 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat HSP90 ELISA Kit (Chemiluminescence) [NBP2-76645] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-76645_rat-hsp90-elisa-kit-chemiluminescence-4320249124812.jpg)