| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89839-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98291-50ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| HRP | NBP2-79615-5Plates | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89838-50ul | |||
![Flow Cytometry: 15-PGDH/HPGD Antibody (016) [NBP2-89839] - Analysis of Human HPGD expression on Jurkat cells. The cells were treated , stained with purified anti-Human HPGD, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-016-Flow-Cytometry-NBP2-89839-img0002.jpg "<b>Biological Strategies Validation. </b>Flow Cytometry: 15-PGDH/HPGD Antibody (016) [NBP2-89839] - Analysis of Human HPGD expression on Jurkat cells. The cells were treated , stained with purified anti-Human HPGD, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: 15-PGDH/HPGD Antibody (016) [NBP2-89839] - Staining of Human HPGD in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89839 antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89839_rabbit-monoclonal-15-pgdh-hpgd-antibody-016-23520251628128.png)
![Western Blot: 15-PGDH/HPGD Antibody [NBP2-98291] - Anti-15-PGDH/HPGD rabbit polyclonal antibody at 1:500 dilution. Lane A: Lovo Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 34 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Western-Blot-NBP2-98291-img0003.jpg "Western Blot: 15-PGDH/HPGD Antibody [NBP2-98291] - Anti-15-PGDH/HPGD rabbit polyclonal antibody at 1:500 dilution. Lane A: Lovo Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 34 kDa (We are unsure as to the identity of these extra bands).")
![Immunoprecipitation: 15-PGDH/HPGD Antibody [NBP2-98291] - Mouse 15-PGDH/HPGD was immunoprecipitated using: Lane A: 0.5 mg Caco-2 Whole Cell Lysate. 2 ul anti-Mouse 15-PGDH/HPGD rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse 15-PGDH/HPGD rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 29 kDa](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Immunoprecipitation-NBP2-98291-img0004.jpg)
![Sandwich ELISA: 15-PGDH/HPGD Antibody Pair [HRP] [NBP2-79615] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.](http://images.novusbio.com/fullsize/15-PGDH-HPGD-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79615-img0001.jpg "Sandwich ELISA: 15-PGDH/HPGD Antibody Pair [HRP] [NBP2-79615] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.")