| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-296SS | |||
| Azide and BSA Free | NBP2-80763 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-74679SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-75978SS | |||
| BSA Free | NBP2-75978 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47178SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47181SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-130SS | |||
| Azide and BSA Free | NBP2-80761 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-69088SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-69089SS | |||
![ELISA HIF-1 alpha [Biotin]](http://images.novusbio.com/fullsize2/dyc1935-2_human-mouse-total-hif-1-alpha-duoset-ic-elisa-5-plate-elisa-10920209831.jpg "ELISA HIF-1 alpha [Biotin]")
![Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - HIF-1 alpha induction by CoCl2 on Caki-1 cell lysate. Image from verified customer review. HIF-1 alpha Antibody (H1alpha67) [NB100-105]](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-905-img0048.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - HIF-1 alpha induction by CoCl2 on Caki-1 cell lysate. Image from verified customer review. HIF-1 alpha Antibody (H1alpha67) [NB100-105]")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody Pack [NB100-905] - HIF-1 alpha was detected in immersion fixed DFO treated Hela cells (left) but was not detected in HIF-1 knockout HeLa cells (right) using Mouse Anti-human HIF-1 alpha monoclonal antibody (Catalog #NB100-105) at 25 ug/mL for 3 hours at room temperature. Cells were stained using a NorthernLights (TM) 557-conjugated Donkey Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. HIF-1 alpha Antibody (H1alpha67) [NB100-105]](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Knockout-Validated-NB100-905-img0049.jpg)
![Chromatin Immunoprecipitation: HIF-1 alpha Antibody Pack [NB100-905] - ChIP-qPCR assay of HIF-1 binding to hypoxia response element (HRE) of the PKM2 gene in human MDA-MB-231 breast cancer cells treated with vehicle or DMOG (HIF inducer) for 8 hours. Negative control: immunoprecipitation was performed with IgG. Image courtesy of Gregg Semenza, Johns Hopkins University School of Medicine. HIF-1 alpha Antibody [NB100-479]](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Chromatin-Immunoprecipitation-NB100-905-img0041.jpg)
![Simple Western: HIF-1 alpha Antibody Pack [NB100-905] - Simple Western lane view shows a specific band for HIF-1 alpha in 0.5 mg/ml of hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. HIF-1 alpha Antibody [NB100-449]](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Simple-Western-NB100-905-img0042.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-905] - HIF-1alpha expression in PDAC and adjacent normal pancreatic tissue. PDAC with weak (intensity 1) nuclear and cytoplasmic HIF-1alpha staining (left). PDAC with strong (intensity 3) nuclear and moderate (intensity 2) cytoplasmic HIF-1alpha staining (right). Image collected and cropped by CiteAb from the following publication (//wjso.biomedcentral.com/articles/10.1186/s12957-018-1432-4), licensed under a CC-BY license. HIF-1 alpha Antibody (H1alpha67) [NB100-105]](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Immunohistochemistry-NB100-905-img0051.jpg)
![Flow Cytometry: HIF-1 alpha Antibody Pack [NB100-905] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-105. Staining of HIF-1 alpha in multiple myeloma cells: H929 cells (0.5x10^6) were stained with Alexa Fluor 488 (R) conjugated HIF-1 alpha antibody (NB100-105AF488). Image courtesy of Dr. Barbara Muz at Washington University in St. Louis School of Medicine. HIF-1 alpha Antibody (H1alpha67) [NB100-105]](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Flow-Cytometry-NB100-905-img0050.jpg)
![<b>Biological Strategies Validation, Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - Effects of in vitro hypoxia on the expression of mouse HIF-1 alpha in HIF-1 alpha+/- and HIF-1 alpha+/+ astrocytes. Confluent astrocyte monolayers of both cell types were exposed to a 6 h in vitro hypoxia. HIF-1 alpha mRNA expression was determined by RT-PCR as described in Materials and Methods. Each bar represents the mean +/- SD of relative density/volumes of the bands on film negatives from at least three experiments. Asterisks and number sign indicate significant difference (p < 0.01; one-way ANOVA, followed by multiple comparisons among means). Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-4-12), licensed under a CC-BY license. HIF-1 alpha Antibody [NB100-654]](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-905-img0053.jpg)
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody Pack [NB100-905] - Detection of HIF-1 alpha (red dye 568) in a cultured raw mouse macrophage cell line, using NB100-131. 100X magnification. Photos courtesy of Susan Alexander and Hattie Gresham, PhD. HIF-1 alpha Antibody (ESEE122) [NB100-131]](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB100-905-img0037.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - Normoxic pre-treatment sensitizes glioma cells to radiation after graded chronic hypoxic (GCH) exposure. The results of anchorage-independent colony forming assays are shown for U87, U87-luc, GL261 glioma cells and 0308 GSCs after 5 Gy radiation exposure under varying O2 conditions. For easier comparisons among cell types, raw values are expressed as a % of the cell type's negative (non-irradiated) control and the means & SEMs are plotted. Also shown are Western blots of nuclear HIF-1alpha at the time of irradiation. Corresponding Western blots of lamin A/C are shown as a loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0111199) licensed under a CC-BY license. HIF-1 alpha Antibody [NB100-479].](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-905-img0040.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - HIF-1 alpha induction on Caki-1 cell lysate using CoCl2. Image from verified customer review. HIF-1 alpha Antibody [NB100-449]](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-905-img0044.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - Effects of CoCl2 treatment on HIF-1 alpha expression in mouse astrocytes. Western blots using nuclear proteins show that both hypoxia (H) and CoCl2 (Co) up-regulated HIF-1 alpha protein in HIF-1 alpha+/+ and HIF-1 alpha+/- cells. There was no HIF-1 alpha protein detected in control cells. Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-4-12), licensed under a CC-BY license. HIF-1 alpha Antibody [NB100-654]](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Western-Blot-NB100-905-img0052.jpg)
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody Pack [NB100-905] - Detection of HIF-1 alpha (red dye) in a cell cytospin from a lavage of a murine skin pouch infected with Staph Aureus, using NB100-131. 100X magnification. Blue dye is DAPI nuclea](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Immunocytochemistry-Immunofluorescence-NB100-905-img0035.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-905] - Staining in normal mouse kidney showing (A) staining with antibody and (B) blocking peptide used at 10-fold excess concentration. Images provided by Dr Yves Heremans. Please see the product review of NBC1-18422 for additional details. HIF-1 alpha Antibody [NB100-479]](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Immunohistochemistry-NB100-905-img0038.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-905] - Hif1alpha expression in preimplantation embryos. Co-staining of OCT4 and Hif1alpha in inner cell mass of blastocyst (top) and negative control images without primary antibodies (bottom). Scale bar: 30um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0154309) licensed under a CC-BY license. HIF-1 alpha Antibody [NB100-479]](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Immunohistochemistry-NB100-905-img0039.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-905] - Immunohistochemical analysis in non-GIST STS representing negative, and score 1-3 of Carbonic Anhydrase IX/CA9, GLUT-1, HIF-1 alpha, and HIF-2 alpha/EPAS1. non-GIST STS: non-gastrointestinal stromal tumor soft-tissue sarcomas, Carbonic Anhydrase IX/CA9: carbonic anhydrase IX, GLUT-1: glucose transporter-1, and HIF-1/2alpha: hypoxia induced factor 1/2alpha. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/sarcoma/2012/541650/), licensed under a CC-BY license. HIF-1 alpha Antibody (ESEE122) [NB100-131]](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Immunohistochemistry-NB100-905-img0045.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody Pack [NB100-905] - Analysis of HIF-1 alpha in paraffin-embedded mouse kidney tissue section using anti-HIF-1 alpha antibody. Image from verified customer review. HIF-1 alpha Antibody (ESEE122) [NB100-131]](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Immunohistochemistry-NB100-905-img0047.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody Pack [NB100-905] - Morphological alterations induced by DMOG are HIF-1alpha dependent. glEND.2 cells were stably transfected with shGFP, shHIF-1alpha or shHIF-2alpha. The clones were cultured under normoxic (N) or hypoxic (H, 1% O2) conditions, or treated with DMOG for 6 h. 30 ug protein of cellular protein were loaded for the detection of HIF-1alpha, and nuclear extracts (20 ug protein) were used for the detection of HIF-2alpha. Image collected and cropped by CiteAb from the following publication (//biosignaling.biomedcentral.com/articles/10.1186/1478-811X-11-80) licensed under a CC-BY license. HIF-1 alpha Antibody [NB100-449]](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-Pack-Knockdown-Validated-NB100-905-img0043.jpg)
![Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (HA111) [NB100-296] - HIF-1 antibody was tested in human ovarian cancer tumor xenograft using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-HA111-Immunohistochemistry-Paraffin-NB100-296-img0001.jpg "Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (HA111) [NB100-296] - HIF-1 antibody was tested in human ovarian cancer tumor xenograft using DAB with hematoxylin counterstain.")
![Western Blot: HIF-1 alpha Antibody (HA111) [NB100-296] - Detection of HIF-1 alpha (125-130 kDa) from human placental villous explant total protein.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-HA111-Western-Blot-NB100-296-img0004.jpg)
![Western Blot: HIF-1 alpha [Hydroxy Pro564] Antibody [NB110-74679] - Detection (left panel) of HIF-1 alpha [Hydroxy Pro564] in nuclear extracts of A549 cells treated with MG132 (a proteasome inhibitor). Hydroxyproline is not recognized on HIF-1alpha when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation. Control staining is shown (right panel) using conventional anti-HIF-1alpha. The asterisk marks a band approximately 110 kDa molecular weight in size corresponding to HIF-1 alpha. The primary antibody was used at a 1:1000 dilution in 2% BLOTTO.](http://images.novusbio.com/fullsize/HIF-1-alpha-[Hydroxy-Pro564]-Antibody-Western-Blot-NB110-74679-img0004.jpg "Western Blot: HIF-1 alpha [Hydroxy Pro564] Antibody [NB110-74679] - Detection (left panel) of HIF-1 alpha [Hydroxy Pro564] in nuclear extracts of A549 cells treated with MG132 (a proteasome inhibitor). Hydroxyproline is not recognized on HIF-1alpha when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation. Control staining is shown (right panel) using conventional anti-HIF-1alpha. The asterisk marks a band approximately 110 kDa molecular weight in size corresponding to HIF-1 alpha. The primary antibody was used at a 1:1000 dilution in 2% BLOTTO.")
![Western Blot: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Western-Blot-NBP2-80765-img0001.jpg "Western Blot: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Immunofluorescent staining of HIF-1 alpha using catalog number NBP2-75977 (clone 2443B). Staining was observed in Hela cells treated with DFO but not in untreated Hela cells. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80765-img0002.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - HIF-1 alpha (clone 2443C) was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (Catalog # NBP2-75978) at 1.0 ug/mL for 1 hour at room temperature followed by incubation with the](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80765-img0003.jpg)
![Simple Western: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Lane view shows a specific band for HIF-1 alpha in HepG2 +/- DFO cell lysate using 20 ug/mL. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 116 KDa. Image from an internal validation. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Simple-Western-NBP2-80765-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (Catalog # NBP2-75978) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha at approximately 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Knockout-Validated-NBP2-80765-img0004.jpg)
![SDS-Page: Recombinant Human HIF-1 alpha Protein [H00003091-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-HIF-1-alpha-Protein-SDS-Page-H00003091-P01-img0002.jpg "SDS-Page: Recombinant Human HIF-1 alpha Protein [H00003091-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: HIF-1 alpha Antibody - Exon 9 [NBP1-47178] - Human recombinant HIF-1 alpha protein.](http://images.novusbio.com/fullsize/HIF-1-alpha-Exon-9-Antibody-exon-9-Western-Blot-NBP1-47178-img0002.jpg "Western Blot: HIF-1 alpha Antibody - Exon 9 [NBP1-47178] - Human recombinant HIF-1 alpha protein.")
![Western Blot: HIF-1 alpha Antibody - Exon 13 [NBP1-47181] - Human recombinant HIF-1 alpha protein.](http://images.novusbio.com/fullsize/HIF-1-alpha-[exon-13]-Antibody-Western-Blot-NBP1-47181-img0002.jpg "Western Blot: HIF-1 alpha Antibody - Exon 13 [NBP1-47181] - Human recombinant HIF-1 alpha protein.")
![Western Blot: HIF-1 alpha Antibody (H1alpha 67-7) [NB100-130] - HIF-1 alpha in 1: Cos7 CoCl2 treated cells. 2: Untreated cells.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-H1alpha-67-7-Western-Blot-NB100-130-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody (H1alpha 67-7) [NB100-130] - HIF-1 alpha in 1: Cos7 CoCl2 treated cells. 2: Untreated cells.")
![Immunoprecipitation: HIF-1 alpha [Monomethyl Lys674] Antibody [NBP2-69088] - Parental or G9a KO HeLa cells were exposed to 1% O2 for 6 h, and subjected to immunoprecipitation with antibodies against K674me1 or K674me2, followed by immunoblot assays with antibodies against HIF-1 alpha, G9a or actin. (Image courtesy of Weibo Luo, UT Southwestern).](http://images.novusbio.com/fullsize/HIF-1-alpha-[Monomethyl-Lys674]-Antibody-Immunoprecipitation-NBP2-69088-img0001.jpg "Immunoprecipitation: HIF-1 alpha [Monomethyl Lys674] Antibody [NBP2-69088] - Parental or G9a KO HeLa cells were exposed to 1% O2 for 6 h, and subjected to immunoprecipitation with antibodies against K674me1 or K674me2, followed by immunoblot assays with antibodies against HIF-1 alpha, G9a or actin. (Image courtesy of Weibo Luo, UT Southwestern).")
![Immunoprecipitation: HIF-1 alpha [Dimethyl Lys674] Antibody [NBP2-69089] - Parental or G9a KO HeLa cells were exposed to 1% O2 for 6 h, and subjected to IP with antibodies against K674me1 or K674me2, followed by immunoblotassays with antibodies against HIF-1 alpha, G9a or actin. Image courtesy of Weibo Luo, UT Southwestern.](http://images.novusbio.com/fullsize/HIF-1-alpha-[Dimethyl-Lys674]-Antibody-Immunoprecipitation-NBP2-69089-img0002.jpg "Immunoprecipitation: HIF-1 alpha [Dimethyl Lys674] Antibody [NBP2-69089] - Parental or G9a KO HeLa cells were exposed to 1% O2 for 6 h, and subjected to IP with antibodies against K674me1 or K674me2, followed by immunoblotassays with antibodies against HIF-1 alpha, G9a or actin. Image courtesy of Weibo Luo, UT Southwestern.")