| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB15291-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11989-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-24217 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| HRP | NBP2-79594-5Plates | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89462-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89463-50ul | |||
![Immunocytochemistry/Immunofluorescence: TIM-3 Antibody (2C12) - Chimeric [NBP3-11989] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 2C12 (NBP3-11989) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11989, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/TIM-3-Antibody-2C12-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-11989-img0001.jpg "Immunocytochemistry/Immunofluorescence: TIM-3 Antibody (2C12) - Chimeric [NBP3-11989] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 2C12 (NBP3-11989) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11989, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![ELISA: TIM-3 Antibody (2C12) - Chimeric - Azide and BSA Free [NBP3-11989] - Binding curves of NBP3-11989 (blue line) and isotype control (NBP2-52638; red line) to an ELISA plate coated with mouse TIM-3-Fc fusion protein at a concentration of 5 ug/ml. A 3-fold serial dilution from 3000 to 0.0169 ng/ml was performed using NBP3-11989. For signal detection, anti-rabbit IgG1 HRP antibody was used.](http://images.novusbio.com/fullsize/nbp3-11989_rabbit-monoclonal-tim-3-antibody-2c12-chimeric-74202522113036.png)
![Flow Cytometry: TIM-3 Antibody (2C12) - Chimeric - Azide and BSA Free [NBP3-11989] - Paraformaldehyde fixed mouse splenocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-11989 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-11989_rabbit-monoclonal-tim-3-antibody-2c12-chimeric-74202522113017.png)
![Western Blot: TIM-3 Overexpression Lysate (Adult Normal) [NBL1-11452] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TIM-3.](http://images.novusbio.com/fullsize/TIM-3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11452-img0002.jpg "Western Blot: TIM-3 Overexpression Lysate (Adult Normal) [NBL1-11452] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TIM-3.")
![SDS-Page: Recombinant Mouse TIM-3 hIgG-His Protein [NBP3-07123] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.](http://images.novusbio.com/fullsize/Recombinant-Mouse-TIM-3-hIgG-His-Protein-SDS-Page-NBP3-07123-img0001.jpg "SDS-Page: Recombinant Mouse TIM-3 hIgG-His Protein [NBP3-07123] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.")
![SDS-Page: Recombinant Human TIM-3 Protein [NBP2-23118] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain](http://images.novusbio.com/fullsize/Recombinant-Human-TIM-3-Protein-SDS-Page-NBP2-23118-img0001.jpg "SDS-Page: Recombinant Human TIM-3 Protein [NBP2-23118] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain")
![Sandwich ELISA: TIM-3 Antibody Pair [HRP] [NBP2-79594] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.](http://images.novusbio.com/fullsize/TIM-3-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79594-img0001.jpg "Sandwich ELISA: TIM-3 Antibody Pair [HRP] [NBP2-79594] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.")