| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12018-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26024 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26036 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26012 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26037 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-2077AF405 | |||
| Alexa Fluor 488 | NB21-2077AF488 | |||
| Alexa Fluor 647 | NB21-2077AF647 | |||
| Alexa Fluor 700 | NB21-2077AF700 | |||
| Biotin | NB21-2077B | |||
| DyLight 488 | NB21-2077G | |||
| DyLight 550 | NB21-2077R | |||
| DyLight 650 | NB21-2077C | |||
| FITC | NB21-2077F | |||
| HRP | NB21-2077H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-2077SS | |||
| BSA Free | NB21-2077 | |||
![Western Blot: Histone H4 [Monomethyl Lys20] Antibody [NBP2-42859] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract 15 % SDS-PAGE Histone H4 (mono methyl Lys20) antibody dilution: 1:1000](http://images.novusbio.com/fullsize/Histone-H4-[Monomethyl-Lys20]-Antibody-Western-Blot-NBP2-42859-img0003.jpg "Western Blot: Histone H4 [Monomethyl Lys20] Antibody [NBP2-42859] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract 15 % SDS-PAGE Histone H4 (mono methyl Lys20) antibody dilution: 1:1000")
![Immunocytochemistry/Immunofluorescence: Histone H4 [Monomethyl Lys20] Antibody [NBP2-42859] - Analysis of HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4 (mono methyl Lys20) protein stained by Histone H4 (mono methyl Lys20) antibody diluted at 1:500. Red: alpha Tubulin antibody [114], a cytoskeleton marker, stained by diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Histone-H4-[Monomethyl-Lys20]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42859-img0002.jpg)
![Western Blot: Histone H4 [ac Lys16] Antibody (RM204) - Azide and BSA Free [NBP3-26012] - Western Blot of acid extracts from: (1) HeLa cells treated with sodium butyrate; (2) HeLa cells untreated, using RM204 at 0.5 ug/mL, showed a band of Histone H4 acetylated at Lysine 16 in HeLa cells.](http://images.novusbio.com/fullsize/nbp3-26012_rabbit-histone-h4-mab-rm204-azide-and-bsa-free-2462024132637.png "Western Blot: Histone H4 [ac Lys16] Antibody (RM204) - Azide and BSA Free [NBP3-26012] - Western Blot of acid extracts from: (1) HeLa cells treated with sodium butyrate; (2) HeLa cells untreated, using RM204 at 0.5 ug/mL, showed a band of Histone H4 acetylated at Lysine 16 in HeLa cells.")
![Multiplex Immunoassay: Histone H4 [ac Lys16] Antibody (RM204) - Azide and BSA Free [NBP3-26012] - RM204 specifically reacts to Histone H4 acetylated at Lysine 16 (K16ac). No cross reactivity with unmodified Lysine 16 (K16 ctrl), acetylated Lysine 5 (K5ac), Lysine 8 (K8ac), Lysine 12 (K12ac), Lysine 20 (K20ac), Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.](http://images.novusbio.com/fullsize/nbp3-26012_rabbit-histone-h4-mab-rm204-azide-and-bsa-free-22620242311017.png)
![Western Blot: Histone H4 [ac Lys12] Antibody (C.15200218) [NBP2-59241] - Histone extracts (15 ug) from HeLa cells were analyzed using the antibody against H4K12ac diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys12-Antibody-C-15200218-Western-Blot-NBP2-59241-img0001.jpg "Western Blot: Histone H4 [ac Lys12] Antibody (C.15200218) [NBP2-59241] - Histone extracts (15 ug) from HeLa cells were analyzed using the antibody against H4K12ac diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.")
![Chromatin Immunoprecipitation: Histone H4 [ac Lys12] Antibody (C.15200218) [NBP2-59241] - ChIP assays were performed using HeLa cells, the monoclonal antibody against H4K12ac and optimized PCR primer sets for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 0.5, 1, 2 and 5 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for the coding region of the EIF2S3 gene, used as a positive control, and for the Sat2 satellite repeat, used as a negative control. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys12-Antibody-C-15200218-Chromatin-Immunoprecipitation-NBP2-59241-img0002.jpg)
![Western Blot: Histone H4 [Methyl Lys20] Antibody (RM208) [NBP2-61552] - Western Blot of acid extracts of HeLa cells (1) and recombinant Histone H4 (2), using NBP2-61552, showed a band of Histone H4 trimethylated at Lysine 20 (K20me3) in HeLa cells.](http://images.novusbio.com/fullsize/Histone-H4-Methyl-Lys20-Antibody-RM208-Western-Blot-NBP2-61552-img0004.jpg "Western Blot: Histone H4 [Methyl Lys20] Antibody (RM208) [NBP2-61552] - Western Blot of acid extracts of HeLa cells (1) and recombinant Histone H4 (2), using NBP2-61552, showed a band of Histone H4 trimethylated at Lysine 20 (K20me3) in HeLa cells.")
![Western Blot: Histone H4 [Monomethyl Lys5] Antibody (RM457) [NBP3-18844] - Western Blot of acid extracts of HeLa cells (2) and recombinant histone H4 (1), using RM457 at 0.2 ug/mL, showed a band of histone H4 monomethylated at Lysine 5 in HeLa cells.](http://images.novusbio.com/fullsize/nbp3-18844_rabbit-histone-h4-mab-rm457-1772024100323.png "Western Blot: Histone H4 [Monomethyl Lys5] Antibody (RM457) [NBP3-18844] - Western Blot of acid extracts of HeLa cells (2) and recombinant histone H4 (1), using RM457 at 0.2 ug/mL, showed a band of histone H4 monomethylated at Lysine 5 in HeLa cells.")
![Western Blot: Histone H4 [ac Lys16] Antibody (RM204) [NBP2-61551] - Western Blot of acid extracts from: (1) HeLa cells treated with sodium butyrate; (2) HeLa cells untreated. Using NBP2-61551, showed a band of Histone H4 acetylated at Lysine 16 in HeLa cells.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys16-Antibody-RM204-Western-Blot-NBP2-61551-img0004.jpg "Western Blot: Histone H4 [ac Lys16] Antibody (RM204) [NBP2-61551] - Western Blot of acid extracts from: (1) HeLa cells treated with sodium butyrate; (2) HeLa cells untreated. Using NBP2-61551, showed a band of Histone H4 acetylated at Lysine 16 in HeLa cells.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [Monomethyl Lys16] Antibody (3E11) [NBP3-15232] - Immunofluorescence staining of Hela cells with Histone H4 antibody at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/Histone-H4-Monomethyl-Lys16-Antibody-3E11-Immunocytochemistry-Immunofluorescence-NBP3-15232-img0001.jpg "Immunocytochemistry/Immunofluorescence: Histone H4 [Monomethyl Lys16] Antibody (3E11) [NBP3-15232] - Immunofluorescence staining of Hela cells with Histone H4 antibody at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).")
![Western Blot: Histone H4 Overexpression Lysate (Adult Normal) [NBL1-11576] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Histone H4.](http://images.novusbio.com/fullsize/Histone-H4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11576-img0002.jpg "Western Blot: Histone H4 Overexpression Lysate (Adult Normal) [NBL1-11576] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Histone H4.")