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![Other Histone H3 [Dimethyl Lys23] Antibody Blocking Peptide](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys23]-Peptide-Chromatin-Immunoprecipitation-NB21-1162PEP-img0002.jpg "Other Histone H3 [Dimethyl Lys23] Antibody Blocking Peptide")
![Other Histone H3 [Asym-dimethyl Arg2] Antibody Blocking Peptide](http://images.novusbio.com/fullsize/Histone-H3-[Asym-dimethyl-Arg2]-Peptide-Chromatin-Immunoprecipitation-NB21-1002PEP-img0002.jpg "Other Histone H3 [Asym-dimethyl Arg2] Antibody Blocking Peptide")
![Other Histone H3 [Monomethyl Lys4] Antibody Blocking Peptide](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys4]-Peptide-Chromatin-Immunoprecipitation-NB21-1021PEP-img0002.jpg "Other Histone H3 [Monomethyl Lys4] Antibody Blocking Peptide")
![Other Histone H3 [Dimethyl Lys4] Antibody Blocking Peptide](http://images.novusbio.com/fullsize/Histone-H3-[Dimethyl-Lys4]-Peptide-Chromatin-Immunoprecipitation-NB21-1022PEP-img0002.jpg "Other Histone H3 [Dimethyl Lys4] Antibody Blocking Peptide")
![Other Histone H3 [Monomethyl Arg2] Antibody Blocking Peptide](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Arg2]-Peptide-Chromatin-Immunoprecipitation-NB21-1001PEP-img0002.jpg "Other Histone H3 [Monomethyl Arg2] Antibody Blocking Peptide")
![Other Histone H3 [Monomethyl Lys36] Antibody Blocking Peptide](http://images.novusbio.com/fullsize/Histone-H3-[Monomethyl-Lys36]-Peptide-Chromatin-Immunoprecipitation-NB21-1251PEP-img0002.jpg "Other Histone H3 [Monomethyl Lys36] Antibody Blocking Peptide")
![Other Histone H3 [Trimethyl Lys18] Antibody Blocking Peptide](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys18]-Peptide-Chromatin-Immunoprecipitation-NB21-1143PEP-img0002.jpg "Other Histone H3 [Trimethyl Lys18] Antibody Blocking Peptide")
![Western Blot: Histone H3 [Trimethyl Lys27] Antibody (BT164) - Chimeric [NBP3-12020] - Nuclear lysate of HeLa(A) (0.0003ug/ml), Jurkat(B) (0.0003ug/ml) and K562(C) (0.01ug/ml) (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of BT164 (NBP3-12020) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys27-Antibody-BT164-Chimeric-Western-Blot-NBP3-12020-img0001.jpg "Western Blot: Histone H3 [Trimethyl Lys27] Antibody (BT164) - Chimeric [NBP3-12020] - Nuclear lysate of HeLa(A) (0.0003ug/ml), Jurkat(B) (0.0003ug/ml) and K562(C) (0.01ug/ml) (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of BT164 (NBP3-12020) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys27] Antibody (BT164) - Chimeric [NBP3-12020] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of BT164 (NBP3-12020) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right, top-bottom NBP3-12020, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/Histone-H3-Trimethyl-Lys27-Antibody-BT164-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12020-img0002.jpg)
![Flow Cytometry: Histone H3 [Trimethyl Lys27] Antibody (BT164) - Chimeric - Azide and BSA Free [NBP3-12020] - HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12020 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-12020_rabbit-monoclonal-histone-h3-trimethyl-lys27-antibody-bt164-chimeric-7420252211303.png)
![Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys23] Antibody (RM169) - Azide and BSA Free [NBP3-26015] - Immunocytochemical staining of HeLa cells treated with sodium butyrate, using Clone RM169 (red). Actin filaments have been labeled with fluorescein phalloidin (green), and nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-26015_rabbit-histone-h3-mab-rm169-azide-and-bsa-free-2462024132618.png "Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys23] Antibody (RM169) - Azide and BSA Free [NBP3-26015] - Immunocytochemical staining of HeLa cells treated with sodium butyrate, using Clone RM169 (red). Actin filaments have been labeled with fluorescein phalloidin (green), and nuclei stained with DAPI (blue).")
![Multiplex Immunoassay: Histone H3 [ac Lys23] Antibody (RM169) - Azide and BSA Free [NBP3-26015] - RM169 specifically reacts to Histone H3 acetylated at Lysine 23. No cross reactivity with unmodified Lysine 23, acetylated Lysine 4, Lysine 9, Lysine 14, Lysine 18, Lysine 27, Lysine 36, Lysine 56, lysine 79, or Lysine 122 in histone H3.](http://images.novusbio.com/fullsize/nbp3-26015_rabbit-histone-h3-mab-rm169-azide-and-bsa-free-22620242217443.png)
![Western Blot: Histone H3 [ac Lys23] Antibody (RM169) - Azide and BSA Free [NBP3-26015] - Western Blot of acid extracts of HeLa cells non-treated (1) or treated with sodium butyrate (2), and recombinant Histone H3.3 (3), using Clone RM169 at 1 ug/mL.](http://images.novusbio.com/fullsize/nbp3-26015_rabbit-histone-h3-mab-rm169-azide-and-bsa-free-226202422321221.png)
![Western Blot: Histone H3 [Trimethyl Lys79] Antibody (RM157) - Azide and BSA Free [NBP3-26002] - Western Blot of recombinant Histone H3.3 (1) and acid extracts of HeLa cells (2), using RM157 at 0.5 ug/mL, showed a band of Histone H3 trimethylated at Lysine 79 in HeLa cells.](http://images.novusbio.com/fullsize/nbp3-26002_rabbit-histone-h3-mab-rm157-azide-and-bsa-free-226202422174412.png "Western Blot: Histone H3 [Trimethyl Lys79] Antibody (RM157) - Azide and BSA Free [NBP3-26002] - Western Blot of recombinant Histone H3.3 (1) and acid extracts of HeLa cells (2), using RM157 at 0.5 ug/mL, showed a band of Histone H3 trimethylated at Lysine 79 in HeLa cells.")
![Dot Blot: Histone H3 [Trimethyl Lys79] Antibody (RM157) - Azide and BSA Free [NBP3-26002] - A Peptide dotblot shows RM157 reacts only to Histone H3 trimethyl-Lysine 79 (K79me3). No cross reactivity with nonmodified Lysine 79 (H3K79Ctrl), monomethylated Lysine 79 (K79me1) or dimethylated Lysine 79 (K79me2).](http://images.novusbio.com/fullsize/nbp3-26002_rabbit-histone-h3-mab-rm157-azide-and-bsa-free-24620241332420.png)
![Multiplex Immunoassay: Histone H3 [Trimethyl Lys79] Antibody (RM157) - Azide and BSA Free [NBP3-26002] - RM157 specifically reacts to Histone H3 trimethylated at Lysine 79 (K79me3). No cross reactivity with other methylated lysines in Histone H3.](http://images.novusbio.com/fullsize/nbp3-26002_rabbit-histone-h3-mab-rm157-azide-and-bsa-free-2462024132615.png)
![Dot Blot: Histone H3 [Trimethyl Lys4] Antibody (RM137) - Azide and BSA Free [NBP3-25986] - A Peptide dotblot shows RM137 reacts only to Histone H3 trimethyl-Lysine 4 (K4me3). No cross reactivity with nonmodified Lysine 4 (H3N1-19), monomethylated Lysine 4 (K4me1) or dimethylated Lysine 4 (K4me2).](http://images.novusbio.com/fullsize/nbp3-25986_rabbit-histone-h3-mab-rm137-azide-and-bsa-free-24620241332444.png "Dot Blot: Histone H3 [Trimethyl Lys4] Antibody (RM137) - Azide and BSA Free [NBP3-25986] - A Peptide dotblot shows RM137 reacts only to Histone H3 trimethyl-Lysine 4 (K4me3). No cross reactivity with nonmodified Lysine 4 (H3N1-19), monomethylated Lysine 4 (K4me1) or dimethylated Lysine 4 (K4me2).")
![Western Blot: Histone H3 [Trimethyl Lys4] Antibody (RM137) - Azide and BSA Free [NBP3-25986] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM137 at 0.5 ug/mL, showed a band of histone H3 trimethylated at Lysine 4 in HeLa cells.](http://images.novusbio.com/fullsize/nbp3-25986_rabbit-histone-h3-mab-rm137-azide-and-bsa-free-24620241332426.png)
![Multiplex Immunoassay: Histone H3 [Trimethyl Lys4] Antibody (RM137) - Azide and BSA Free [NBP3-25986] - RM137 specifically reacts to Histone H3 trimethylated at Lysine 4 (K4me3). No cross reactivity with other methylated lysines in Histone H3.](http://images.novusbio.com/fullsize/nbp3-25986_rabbit-histone-h3-mab-rm137-azide-and-bsa-free-2462024132622.png)
![Western Blot: Histone H3 [Trimethyl Lys36] Antibody (RM155) - Azide and BSA Free [NBP3-25988] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM155 at 2 ug/mL.](http://images.novusbio.com/fullsize/nbp3-25988_rabbit-histone-h3-mab-rm155-azide-and-bsa-free-22620242217447.png "Western Blot: Histone H3 [Trimethyl Lys36] Antibody (RM155) - Azide and BSA Free [NBP3-25988] - Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM155 at 2 ug/mL.")
![Multiplex Immunoassay: Histone H3 [Trimethyl Lys36] Antibody (RM155) - Azide and BSA Free [NBP3-25988] - RM155 specifically reacts to Histone H3 trimethylated at Lysine 36 (K36me3). No cross reactivity with non-modified Lysine 36 (K36 Ctrl), monomethylated Lysine 36 (K36me1) or dimethylated Lysine 36 (K36me2), or other methylations in Histone H3.](http://images.novusbio.com/fullsize/nbp3-25988_rabbit-histone-h3-mab-rm155-azide-and-bsa-free-246202413263.png)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys36] Antibody (RM155) - Azide and BSA Free [NBP3-25988] - ChIP performed on HeLa cells using RM155, 5ug. Real-time PCR was performed using primers specific to the gene indicated.](http://images.novusbio.com/fullsize/nbp3-25988_rabbit-histone-h3-mab-rm155-azide-and-bsa-free-246202413269.png)