| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26025 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26026 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26045 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-58444-25ul | |||
![Immunocytochemistry/Immunofluorescence: Histone H2A.Z Antibody (ST46-09) [NBP2-67499] - Staining of Histone H2A.Z in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H2A-Z-Antibody-ST46-09-Immunocytochemistry-Immunofluorescence-NBP2-67499-img0006.jpg "Immunocytochemistry/Immunofluorescence: Histone H2A.Z Antibody (ST46-09) [NBP2-67499] - Staining of Histone H2A.Z in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).")
![Immunohistochemistry-Paraffin: Histone H2A.Z Antibody (ST46-09) [NBP2-67499] - Human kidney tissue with Rabbit anti-Histone H2A.Z antibody at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Histone-H2A-Z-Antibody-ST46-09-Immunohistochemistry-Paraffin-NBP2-67499-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H2A.Z Antibody (ST46-09) [NBP2-67499] - Staining Histone H2A.Z in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Histone-H2A-Z-Antibody-ST46-09-Immunocytochemistry-Immunofluorescence-NBP2-67499-img0001.jpg)
![Immunohistochemistry-Paraffin: Histone H2A.Z Antibody (ST46-09) [NBP2-67499] - Analysis of paraffin-embedded human kidney tissue using anti-Histone H2A.Z antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Histone-H2A-Z-Antibody-ST46-09-Immunohistochemistry-Paraffin-NBP2-67499-img0002.jpg)
![Immunohistochemistry-Paraffin: Histone H2A.Z Antibody (ST46-09) [NBP2-67499] - Analysis of paraffin-embedded human lung cancer tissue using anti-Histone H2A.Z antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Histone-H2A-Z-Antibody-ST46-09-Immunohistochemistry-Paraffin-NBP2-67499-img0003.jpg)
![Immunohistochemistry-Paraffin: Histone H2A.Z Antibody (ST46-09) [NBP2-67499] - Analysis of paraffin-embedded human spleen tissue using anti-Histone H2A.Z antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Histone-H2A-Z-Antibody-ST46-09-Immunohistochemistry-Paraffin-NBP2-67499-img0004.jpg)
![Immunohistochemistry-Paraffin: Histone H2A.Z Antibody (ST46-09) [NBP2-67499] - Analysis of paraffin-embedded mouse colon tissue using anti-Histone H2A.Z antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Histone-H2A-Z-Antibody-ST46-09-Immunohistochemistry-Paraffin-NBP2-67499-img0005.jpg)
![Immunohistochemistry-Paraffin: Histone H2A.Z Antibody (ST46-09) [NBP2-67499] - Human liver tissue with Rabbit anti-Histone H2A.Z antibody at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Histone-H2A-Z-Antibody-ST46-09-Immunohistochemistry-Paraffin-NBP2-67499-img0007.jpg)
![Western Blot: Histone H2A.Z [ac Lys7, ac Lys11, ac Lys4] Antibody [NBP2-54619] - H2AZ [ac Lys4, ac Lys7, ac Lys11] Antibody [NBP2-54619] - Western blot was performed on whole cell extracts (25 ug, lane 1) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 2, 3, 4 and 5, respectively) using the antibody against H2A.Zac. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right, the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/H2AZ-[ac-Lys4--ac-Lys7--ac-Lys11]-Antibody-Western-Blot-NBP2-54619-img0008.jpg "Western Blot: Histone H2A.Z [ac Lys7, ac Lys11, ac Lys4] Antibody [NBP2-54619] - H2AZ [ac Lys4, ac Lys7, ac Lys11] Antibody [NBP2-54619] - Western blot was performed on whole cell extracts (25 ug, lane 1) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 2, 3, 4 and 5, respectively) using the antibody against H2A.Zac. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right, the marker (in kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: Histone H2A.Z [ac Lys7, ac Lys11, ac Lys4] Antibody [NBP2-54619] - H2AZ [ac Lys4, ac Lys7, ac Lys11] Antibody [NBP2-54619] - HeLa cells were stained with the antibody against H2A.Zac and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H2AZac antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/H2AZ-[ac-Lys4--ac-Lys7--ac-Lys11]-Antibody-Immunofluorescence-NBP2-54619-img0009.jpg)
![Chromatin Immunoprecipitation: Histone H2A.Z [ac Lys7, ac Lys11, ac Lys4] Antibody [NBP2-54619] - H2AZ [ac Lys7, ac Lys11, ac Lys4] Antibody [NBP2-54619] - H2AZ [ac Lys4, ac Lys7, ac Lys11] Antibody [NBP2-54619] - ChIP assays were performed using human K562 cells, the antibody against H2AZac and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 100,000 cells. A titration of the antibody consisting of 0.2, 0.5, 1 and 2 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. Quantitative PCR was performed with primers specific for the promoter of the active genes CCT5 and EIF4A2, used as positive controls, and for the coding region of the inactive MYT1 gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a percent of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis](http://images.novusbio.com/fullsize/H2AZ-[ac-Lys4--ac-Lys7--ac-Lys11]-Antibody-Chromatin-Immunoprecipitation-NBP2-54619-img0005.jpg)
![Chromatin Immunoprecipitation: Histone H2A.Z [ac Lys7, ac Lys11, ac Lys4] Antibody [NBP2-54619] - H2AZ [ac Lys7, ac Lys11, ac Lys4] Antibody [NBP2-54619] - H2AZ [ac Lys4, ac Lys7, ac Lys11] Antibody [NBP2-54619] - ChIP assays were performed using human HeLa cells, the antibody against H2A.Zac and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1,000,000 cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control.](http://images.novusbio.com/fullsize/H2AZ-ac-Lys4-ac-Lys7-ac-Lys11-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-54619-img0001.jpg)
![ELISA: Histone H2A.Z [ac Lys7, ac Lys11, ac Lys4] Antibody [NBP2-54619] - H2AZ [ac Lys4, ac Lys7, ac Lys11] Antibody [NBP2-54619] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against H2A.Zac. The antigen used was a peptide containing the histone modifications of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:56,600.](http://images.novusbio.com/fullsize/H2AZ-[ac-Lys4--ac-Lys7--ac-Lys11]-Antibody-ELISA-NBP2-54619-img0006.jpg)
![Dot Blot: Histone H2A.Z [ac Lys7, ac Lys11, ac Lys4] Antibody [NBP2-54619] - H2AZ [ac Lys4, ac Lys7, ac Lys11] Antibody [NBP2-54619] - To test the cross reactivity of the antibody against H2A.Zac, a Dot Blot analysis was performed with peptides containing different multiple acetylations and the unmodified H2A.Z. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/H2AZ-[ac-Lys4--ac-Lys7--ac-Lys11]-Antibody-Dot-Blot-NBP2-54619-img0007.jpg)
![Western Blot: Histone H2A.Z [ac Lys7, ac Lys11, ac Lys4] Antibody [NBP3-18689] - Figure 5. Western blot analysis using the antibody directed against Histone H2A.Z Western blot was performed on whole cell extracts (25 ug, lane 1) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 2, 3, 4 and 5, respectively) using the antibody against Histone H2A.Z . The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right, the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-H2A.Z-[ac-Lys7--ac-Lys11--ac-Lys4]-Antibody-Western-Blot-NBP3-18689-img0001.jpg "Western Blot: Histone H2A.Z [ac Lys7, ac Lys11, ac Lys4] Antibody [NBP3-18689] - Figure 5. Western blot analysis using the antibody directed against Histone H2A.Z Western blot was performed on whole cell extracts (25 ug, lane 1) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 2, 3, 4 and 5, respectively) using the antibody against Histone H2A.Z . The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right, the marker (in kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: Histone H2A.Z [ac Lys7, ac Lys11, ac Lys4] Antibody [NBP3-18689] - Figure 6. Immunofluorescence using the antibody directed against Histone H2A.Z HeLa cells were stained with the antibody against Histone H2A.Z and with DAPI. Cells were fixed with 4% formaldehyde for 10 and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the Histone H2A.Z antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone H2A.Z [ac Lys7- ac Lys11- ac Lys4] Antibody-Immunocytochemistry-Immunofluorescence-NBP3-18689-img0006.jpg)
![Chromatin Immunoprecipitation Sequencing: Histone H2A.Z [ac Lys7, ac Lys11, ac Lys4] Antibody [NBP3-18689] - Figure 2. ChIP-seq results obtained with the antibody directed against Histone H2A.Z ChIP was performed with 0.5 ug of the antibody against Histone H2A.Z as described above. The IP'd DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution along the complete sequence and a 1 Mb region of human chromosome 1 (figure 2A and B) and in two regions surrounding the GAPDH and the EIF4A2 positive control gene (figure 2C and D, respectively).](http://images.novusbio.com/fullsize/Histone-H2A.Z-[ac-Lys7--ac-Lys11--ac-Lys4]-Antibody-Chromatin-Immunoprecipitation-Sequencing-NBP3-18689-img0004.jpg)
![Chromatin Immunoprecipitation Sequencing: Histone H2A.Z [ac Lys7, ac Lys11, ac Lys4] Antibody [NBP3-18689] - Figure 1. ChIP results obtained with the antibody directed against Histone H2A.Z ChIP assays were performed using HeLa cells, the antibody against Histone H2A.Z and optimized primer pairs for qPCR. ChIP was performed on sheared chromatin from 100,000 K562 cells using the iDeal ChIP-seq kit. A titration of the antibody consisting of 0.2, 0.5, 1 and 2 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed using primers specific for the promoter of the EIF4A2 gene, used as positive control target and for the coding region of the MYT1 gene, and the Sat2 satellite repeat, used as negative control targets. Figure 1 shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA).](http://images.novusbio.com/fullsize/Histone-H2A.Z-[ac-Lys7--ac-Lys11--ac-Lys4]-Antibody-Chromatin-Immunoprecipitation-Sequencing-NBP3-18689-img0003.jpg)
![ELISA: Histone H2A.Z [ac Lys7, ac Lys11, ac Lys4] Antibody [NBP3-18689] - Figure 3. Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against Histone H2A.Z . The antigen used was a peptide containing the histone modifications of interest. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the purified antibody was estimated to be 1:265,000.](http://images.novusbio.com/fullsize/Histone-H2A.Z-[ac-Lys7--ac-Lys11--ac-Lys4]-Antibody-ELISA-NBP3-18689-img0002.jpg)
![Dot Blot: Histone H2A.Z [ac Lys7, ac Lys11, ac Lys4] Antibody [NBP3-18689] - Figure 4. Cross reactivity test using the antibody directed against Histone H2A.Z A Dot Blot analysis was performed to test the cross reactivity of the antibody against Histone H2A.Z with peptides containing other histone acetylations and the unmodified H2A.Z sequence. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure 4 shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H2A.Z-[ac-Lys7--ac-Lys11--ac-Lys4]-Antibody-Dot-Blot-NBP3-18689-img0005.jpg)
![Western Blot: H2AZ Antibody [NBP2-54618] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 5, 6, 7 and 8, respectively) using the antibody against H2A.Z. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Alternatively, Western blot was performed on histone extracts after incubation of the antibody with 1 ug of the peptide used for immunisation of the rabbit (1 hour at room temperature) (lane 3) or with a peptide containing a sequence from the central part of the H2A.Z protein (lane 4). The position of the protein of interest is indicated on the right, the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/H2AZ-Antibody-Western-Blot-NBP2-54618-img0005.jpg "Western Blot: H2AZ Antibody [NBP2-54618] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 5, 6, 7 and 8, respectively) using the antibody against H2A.Z. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Alternatively, Western blot was performed on histone extracts after incubation of the antibody with 1 ug of the peptide used for immunisation of the rabbit (1 hour at room temperature) (lane 3) or with a peptide containing a sequence from the central part of the H2A.Z protein (lane 4). The position of the protein of interest is indicated on the right, the marker (in kDa) is shown on the left.")
![Chromatin Immunoprecipitation: H2AZ Antibody [NBP2-54618] - ChIP assays were performed using human K562 cells, the antibody against H2A.Z and optimized PCR primer sets for qPCR. ChIP was performed with sheared chromatin from 100,000 cells. A titration of the antibody consisting of 0.2, 0.5, 1 and 2ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. Quantitative PCR was performed with primers specific for the promoter of the active genes c-fos and EIF2S3, used as positive controls, and for the coding region of the inactive MB gene and the Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/H2AZ-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-54618-img0003.jpg)
![Chromatin Immunoprecipitation: H2AZ Antibody [NBP2-54618] - ChIP assays were performed using human HeLa cells, the antibody against H2A.Z and optimized PCR primer pairs for qPCR. ChIP was performed using sheared chromatin from 1,000,000 cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter of the active genes c-fos and EIF2S3, used as positive controls, and for the inactive TSH2B gene and the Sat2 satellite repeat, used as negative controls.](http://images.novusbio.com/fullsize/H2AZ-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-54618-img0001.jpg)
![ELISA: H2AZ Antibody [NBP2-54618] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against H2A.Z. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:87,500.](http://images.novusbio.com/fullsize/H2AZ-Antibody-ELISA-NBP2-54618-img0004.jpg)
![Immunofluorescence: H2AZ Antibody [NBP2-54618] - HeLa cells were stained with the antibody against H2A.Z. and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. Top Figure: cells were immunofluorescently labeled with the H2A.Z antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right. Middle Figure and lower Figure: staining of the cells with the H2A.Z antibody after incubation of the antibody with 10 ng/ul of the peptide used for immunisation of the rabbit (middle Figure) and with a peptide containing a sequence from the central part of the H2A.Z protein (lower Figure).](http://images.novusbio.com/fullsize/H2AZ-Antibody-Immunofluorescence-NBP2-54618-img0006.jpg)
![Western Blot: Histone H2A.Z Antibody (2X2I10) [NBP3-16447] - Western blot analysis of extracts of various cell lines, using Histone H2A.Z Rabbit mAb (NBP3-16447) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Histone-H2A-Z-Antibody-2X2I10-Western-Blot-NBP3-16447-img0001.jpg "Western Blot: Histone H2A.Z Antibody (2X2I10) [NBP3-16447] - Western blot analysis of extracts of various cell lines, using Histone H2A.Z Rabbit mAb (NBP3-16447) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Chromatin Immunoprecipitation: Histone H2A.Z Antibody (2X2I10) [NBP3-16447] - Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Histone H2A.Z Rabbit mAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-16447_rabbit-histone-h2a-z-mab-2x2i10-522025125434.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Histone H2A.Z Antibody (2X2I10) [NBP3-16447] - Western blot analysis of lysates from wild type(WT) and Histone H2A.Z knockout (KO) 293T(KO) cells, using [KO Validated] Histone H2A.Z Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-16447_rabbit-histone-h2a-z-mab-2x2i10-52202511365196.jpg)
![Chromatin Immunoprecipitation: Histone H2A.Z Antibody (2X2I10) [Histone H2A.Z] - Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Histone H2A.Z Rabbit mAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-16447_rabbit-histone-h2a-z-mab-2x2i10-52202517152730.jpg)
![Multiplex Immunoassay: Histone H2A.Z [ac Lys4] Antibody (RM221) - Azide and BSA Free [NBP3-26025] - RM221 specifically reacts to Histone H2A.Z acetylated at Lysine 4. No cross reactivity with non-modified Lysine 4 or other acetylated Lysines in histone H2A.](http://images.novusbio.com/fullsize/nbp3-26025_rabbit-histone-h2a-z-mab-rm221-azide-and-bsa-free-24620241332429.png "Multiplex Immunoassay: Histone H2A.Z [ac Lys4] Antibody (RM221) - Azide and BSA Free [NBP3-26025] - RM221 specifically reacts to Histone H2A.Z acetylated at Lysine 4. No cross reactivity with non-modified Lysine 4 or other acetylated Lysines in histone H2A.")
![Western Blot: Histone H2A.Z [ac Lys4] Antibody (RM221) - Azide and BSA Free [NBP3-26025] - Western Blot of acid extracts from HeLa cells treated (+) or untreated (-) with sodium butyrate, using Clone RM221 at 0.5 ug/mL.](http://images.novusbio.com/fullsize/nbp3-26025_rabbit-histone-h2a-z-mab-rm221-azide-and-bsa-free-226202422174420.png)
![Immunocytochemistry/ Immunofluorescence: Histone H2A.Z [ac Lys4] Antibody (RM221) - Azide and BSA Free [NBP3-26025] - Immunocytochemical staining of HeLa cells treated with sodium butyrate, using Clone RM221 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp3-26025_rabbit-histone-h2a-z-mab-rm221-azide-and-bsa-free-2462024124595.png)
![Western Blot: Histone H2A.Z [ac Lys4] Antibody (RM221) [NBP2-61483] - Western Blot of acid extracts from HeLa cells treated (+) or untreated (-) with sodium butyrate, using NBP2-61483.](http://images.novusbio.com/fullsize/Histone-H2A-Z-ac-Lys4-Antibody-RM221-Western-Blot-NBP2-61483-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H2A.Z [ac Lys4] Antibody (RM221) [NBP2-61483] - Western Blot of acid extracts from HeLa cells treated (+) or untreated (-) with sodium butyrate, using NBP2-61483.")
![<b>Biological Strategies Validation. </b>Multiplex Immunoassay: Histone H2A.Z [ac Lys4] Antibody (RM221) - Azide and BSA Free [NBP2-61483] - RM221 specifically reacts to Histone H2A.Z acetylated at Lysine 4. No cross reactivity with non-modified Lysine 4 or other acetylated Lysines in histone H2A.](http://images.novusbio.com/fullsize/nbp2-61483_rabbit-monoclonal-histone-h2a-z-ac-lys4-antibody-rm221-multiplex-immunoassay-18920252339434.png)
![Immunocytochemistry/ Immunofluorescence: Histone H2A.Z [ac Lys4] Antibody (RM221) - Azide and BSA Free [NBP2-61483] - Immunocytochemical staining of HeLa cells treated with sodium butyrate, using Clone RM221 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp2-61483_rabbit-monoclonal-histone-h2a-z-ac-lys4-antibody-rm221-immunocytochemistry-immunofluorescence-189202523435610.png)
![Western Blot: Histone H2A.Z [ac Lys7] Antibody (RM222) [NBP2-61484] - Western Blot of acid extracts from HeLa cells treated (+) or untreated (-) with sodium butyrate, using NBP2-61484.](http://images.novusbio.com/fullsize/Histone-H2A-Z-ac-Lys7-Antibody-RM222-Western-Blot-NBP2-61484-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H2A.Z [ac Lys7] Antibody (RM222) [NBP2-61484] - Western Blot of acid extracts from HeLa cells treated (+) or untreated (-) with sodium butyrate, using NBP2-61484.")
![<b>Biological Strategies Validation. </b>Multiplex Immunoassay: Histone H2A.Z [ac Lys7] Antibody (RM222) - Azide and BSA Free [NBP2-61484] - RM222 specifically reacts to Histone H2A.Z acetylated at Lysine 7. No cross reactivity with non-modified Lysine 7 or other acetylated Lysines in histone H2A.](http://images.novusbio.com/fullsize/nbp2-61484_rabbit-monoclonal-histone-h2a-z-ac-lys7-antibody-rm222-multiplex-immunoassay-189202523521118.png)
![Immunocytochemistry/ Immunofluorescence: Histone H2A.Z [ac Lys7] Antibody (RM222) - Azide and BSA Free [NBP2-61484] - Immunocytochemical staining of HeLa cells treated with sodium butyrate, using Clone RM222 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp2-61484_rabbit-monoclonal-histone-h2a-z-ac-lys7-antibody-rm222-immunocytochemistry-immunofluorescence-189202523521124.png)
![Western Blot: Histone H2A.Z Antibody (RM215) [NBP2-61488] - Western Blot of HeLa, HEK293, A375, SK-MEL-2 and A431 whole cell lysates, using NBP2-61488, showed endogenous Histone H2AZ in HeLa, HEK293, A375, SK-MEL-2 and A431 cells.](http://images.novusbio.com/fullsize/Histone-H2A-Z-Antibody-RM215-Western-Blot-NBP2-61488-img0003.jpg "Western Blot: Histone H2A.Z Antibody (RM215) [NBP2-61488] - Western Blot of HeLa, HEK293, A375, SK-MEL-2 and A431 whole cell lysates, using NBP2-61488, showed endogenous Histone H2AZ in HeLa, HEK293, A375, SK-MEL-2 and A431 cells.")
![Immunocytochemistry/ Immunofluorescence: Histone H2A.Z Antibody (RM215) - Azide and BSA Free [NBP2-61488] - Immunocytochemistry of HeLa cells, using Clone RM215 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp2-61488_rabbit-monoclonal-histone-h2a-z-antibody-rm215-immunocytochemistry-immunofluorescence-18920252341545.png)
![Immunohistochemistry-Paraffin: Histone H2A.Z Antibody [NBP2-58444] - Immunohistochemical staining of human tonsil shows nuclear positivity in germinal center and non-germinal center cells.](http://images.novusbio.com/fullsize/Histone-H2A.Z-Antibody-Immunohistochemistry-Paraffin-NBP2-58444-img0001.jpg "Immunohistochemistry-Paraffin: Histone H2A.Z Antibody [NBP2-58444] - Immunohistochemical staining of human tonsil shows nuclear positivity in germinal center and non-germinal center cells.")
![Western Blot: Histone H2A.Z Antibody (S09-3H9) [NBP3-14879] - Western blot detection of Histone H2A. Z in K562, C6, 3T3 cell lysates using Histone H2A. Z Rabbit mAb (1:1000 diluted). Predicted band size: 14kDa. Observed band size: 14kDa.](http://images.novusbio.com/fullsize/Histone-H2A-Z-Antibody-S09-3H9-Western-Blot-NBP3-14879-img0001.jpg "Western Blot: Histone H2A.Z Antibody (S09-3H9) [NBP3-14879] - Western blot detection of Histone H2A. Z in K562, C6, 3T3 cell lysates using Histone H2A. Z Rabbit mAb (1:1000 diluted). Predicted band size: 14kDa. Observed band size: 14kDa.")
![Western Blot: Histone H2A.Z Overexpression Lysate (Adult Normal) [NBL1-11427] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Histone H2A.Z.](http://images.novusbio.com/fullsize/Histone-H2A-Z-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11427-img0002.jpg "Western Blot: Histone H2A.Z Overexpression Lysate (Adult Normal) [NBL1-11427] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Histone H2A.Z.")