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![Flow Cytometry: Granzyme B Antibody (B18.1) [Alexa Fluor® 647] [NBP1-97525AF647] - An intracellular stain was performed on U937 cells with Granzyme B [B18.1] Antibody NBP1-97525AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Granzyme-B-Antibody-B18-1-Alexa-Fluor-R-647-Flow-Cytometry-NBP1-97525AF647-img0001.jpg "Flow Cytometry: Granzyme B Antibody (B18.1) [Alexa Fluor® 647] [NBP1-97525AF647] - An intracellular stain was performed on U937 cells with Granzyme B [B18.1] Antibody NBP1-97525AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.")