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![Western Blot: EGFR [p Thr669] Antibody [NBP3-13264] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Thr669) antibody (NBP3-13264) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EGFR-[p-Thr669]-Antibody-Western-Blot-NBP3-13264-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: EGFR [p Thr669] Antibody [NBP3-13264] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Thr669) antibody (NBP3-13264) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: EGFR [p Thr669] Antibody (GT1361) [NBP3-13645] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Thr669) antibody [GT1361] (NBP3-13645) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/EGFR-p-Thr669-Antibody-GT1361-Western-Blot-NBP3-13645-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: EGFR [p Thr669] Antibody (GT1361) [NBP3-13645] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR (phospho Thr669) antibody [GT1361] (NBP3-13645) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Sandwich ELISA: EGFR Antibody Pair [HRP] [NBP2-79430] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.](http://images.novusbio.com/fullsize/EGFR-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79430-img0001.jpg "Sandwich ELISA: EGFR Antibody Pair [HRP] [NBP2-79430] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.")
![Western Blot: EGFR [p Tyr845] Antibody (JE46-77) [NBP3-32761] - Western blot analysis of EGFR on different lysates with Rabbit anti-EGFR antibody (NBP3-32761) at 1/1,000 dilution. Lane 1: A431 whole cell lysate Lane 2: A431 treated with 100ng/mL EGF for 10 minutes whole cell lysate Lane 3: A431 whole cell lysate treated with pp for 1 hour Lane 4: A431 treated with 100ng/mL EGF for 10 minutes whole cell lysate treated with pp for 1 hour Lysates/proteins at 20 ug/Lane. Predicted band size: 134 kDa Observed band size: 180/75 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32761) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32761_rabbit-egfr-p-tyr845-mab-je46-77-28820241244290.jpg "Western Blot: EGFR [p Tyr845] Antibody (JE46-77) [NBP3-32761] - Western blot analysis of EGFR on different lysates with Rabbit anti-EGFR antibody (NBP3-32761) at 1/1,000 dilution. Lane 1: A431 whole cell lysate Lane 2: A431 treated with 100ng/mL EGF for 10 minutes whole cell lysate Lane 3: A431 whole cell lysate treated with pp for 1 hour Lane 4: A431 treated with 100ng/mL EGF for 10 minutes whole cell lysate treated with pp for 1 hour Lysates/proteins at 20 ug/Lane. Predicted band size: 134 kDa Observed band size: 180/75 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32761) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")