| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1019-SP | |||
F0113). View our protocol for Staining Membrane-associated Proteins." alt="RAW 264.7 mouse monocyte/macrophage cell line either treated with LPS overnight (filled histogram) or untreated (open historgram) was stained with Rat Anti-Mouse PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB9078), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | FAB9078T-100UG | |||
| Carrier Free | MAB9078-SP | |||
| Carrier Free | FAB9078R-025 | |||
| Carrier Free | FAB9078V-100UG | |||
| Carrier Free | FAB9078U-100UG | |||
| Carrier Free | FAB9078S-100UG | |||
| Carrier Free | FAB9078N-100UG | |||
| Carrier Free | FAB9078G-100UG | |||
DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." alt="PD-L1 was detected in immersion fixed paraffin-embedded sections of human Lung Tumor using Rabbit Anti-Human PD-L1, Monoclonal Antibody (Catalog #NBP2-23740) at 5ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-24107 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20876 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-21060 | |||
MAB006) followed by Phycoerythrin-conjugated Anti-Hamster IgG Secondary Antibody ." class="big_thumb" />
1021-PD) with an ED50 of 8-40 ng/mL." alt="When Recombinant Mouse PD-L1/B7-H1 is immobilize at 25 ng/mL, it binds Recombinant Mouse PD-1 Fc Chimera (Catalog # 
![Immunohistochemistry: PD-L1 Antibody (4V8L4) [PD-L1] - Immunohistochemistry analysis of paraffin-embedded Human placenta using PD-L1 Rabbit mAb at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15686_rabbit-pd-l1-mab-4v8l4-5220251734540.jpg "Immunohistochemistry: PD-L1 Antibody (4V8L4) [PD-L1] - Immunohistochemistry analysis of paraffin-embedded Human placenta using PD-L1 Rabbit mAb at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Immunohistochemistry: PD-L1 Antibody (4V8L4) [PD-L1] - Immunohistochemistry analysis of paraffin-embedded Human tonsil using PD-L1 Rabbit mAb at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15686_rabbit-pd-l1-mab-4v8l4-52202517315539.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: PD-L1 Antibody (4V8L4) [PD-L1] - Western blot analysis of lysates from wild type (WT) and PD-L1 knockout (KO) A-549 cells using PD-L1 Rabbit mAb at 1:1000 dilution incubated overnight at 4C. Wild type (WT) and PD-L1 knockout (KO) A549 cells were treated by IFNG (100 ng/mL) at 37C for 48 hours.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 30 ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time: 45s.](http://images.novusbio.com/fullsize/nbp3-15686_rabbit-pd-l1-mab-4v8l4-52202517315579.jpg)
![Immunohistochemistry: PD-L1 Antibody (4V8L4) [PD-L1] - Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma using PD-L1 Rabbit mAb at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15686_rabbit-pd-l1-mab-4v8l4-52202517311099.jpg)
![Immunocytochemistry/Immunofluorescence: PD-L1 Antibody (F2G2) - VHH [NBP3-12826] - Immunofluorescence of PD-L1 in transfected 293 cells with PD-L1 antibody at 10 ug/ml. Green: PD-L1 Antibody [F2G2] Blue: DAPI staining](http://images.novusbio.com/fullsize/PD-L1-Antibody-F2G2-VHH-Immunocytochemistry-Immunofluorescence-NBP3-12826-img0002.jpg "Immunocytochemistry/Immunofluorescence: PD-L1 Antibody (F2G2) - VHH [NBP3-12826] - Immunofluorescence of PD-L1 in transfected 293 cells with PD-L1 antibody at 10 ug/ml. Green: PD-L1 Antibody [F2G2] Blue: DAPI staining")
![Immunocytochemistry/Immunofluorescence: PD-L1 Antibody (F2G2) - VHH [NBP3-12826] - Immunocytochemistry of PD-L1 in transfected 293 cells with PD-L1 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/PD-L1-Antibody-F2G2-VHH-Immunocytochemistry-Immunofluorescence-NBP3-12826-img0003.jpg)
![ELISA: PD-L1 Antibody (F2G2) - VHH [NBP3-12826] - Titration ELISA of PD-L1.](http://images.novusbio.com/fullsize/PD-L1-Antibody-F2G2-VHH-ELISA-NBP3-12826-img0001.jpg)
![Immunohistochemistry: PD-L1 Antibody (PSH04-80) [NBP3-32731] - Immunohistochemical analysis of paraffin-embedded mouse thymus tissue with Rabbit anti-PD-L1 antibody (NBP3-32731) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32731) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32731_rabbit-pd-l1-mab-psh04-80-29820241433368.jpg "Immunohistochemistry: PD-L1 Antibody (PSH04-80) [NBP3-32731] - Immunohistochemical analysis of paraffin-embedded mouse thymus tissue with Rabbit anti-PD-L1 antibody (NBP3-32731) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32731) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western blot analysis on different lysates with NBP3-32731 at 1/2,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 10μg/mL LPS for 8 hours cell lysate Lane 3: J774A.1 cell lysate Lane 4: J774A.1 treated with 1μg/mL LPS for 24 hours cell lysate Lane 5: Mouse spleen tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 33 kDa Observed band size: 45-60 kDa Exposure time: 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32731 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32731_-western-blot-639204597489106834.jpg)
![Immunohistochemistry: PD-L1 Antibody (PD01-02) [NBP3-32732] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-PD-L1 antibody (NBP3-32732) at 1/800 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32732) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32732_rabbit-pd-l1-mab-pd01-02-29820241492673.jpg "Immunohistochemistry: PD-L1 Antibody (PD01-02) [NBP3-32732] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-PD-L1 antibody (NBP3-32732) at 1/800 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32732) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western blot analysis on different lysates with NBP3-32732 at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: MDA-MB-231 cell lysate Lane 2: MCF7 cell lysate (negative) Lane 3: U-87 MG cell lysate Lane 4: HepG2 cell lysate (low expression) Lane 5: HeLa cell lysate Lane 6: A549 cell lysate Lane 7: A549 treated with 100ng/mL IFN gamma for 48 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 33 kDa Observed band size: 45-50 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32732 at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32732_-western-blot-639204594550581899.jpg)
