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![Western Blot: CDC2/CDK1 [p Thr14] Antibody [NBP3-13300] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CDC2/CDK1 (phospho Thr14) antibody (NBP3-13300) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CDC2-CDK1-[p-Thr14]-Antibody-Western-Blot-NBP3-13300-img0002.jpg "Western Blot: CDC2/CDK1 [p Thr14] Antibody [NBP3-13300] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CDC2/CDK1 (phospho Thr14) antibody (NBP3-13300) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: CDC2/CDK1 [p Thr14] Antibody [NBP3-13300] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CDC2/CDK1 (phospho Thr14) antibody (NBP3-13300) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CDC2-CDK1-[p-Thr14]-Antibody-Western-Blot-NBP3-13300-img0001.jpg)
![Western Blot: CDC2/CDK1 [p Thr14] Antibody (6R1O7) [NBP3-16913] - Analysis of extracts of various cell lines, using CDC2/CDK1 Rabbit mAb (NBP3-16913) at 1:1000 dilution.HeLa cells and NIH/3T3 cells and C6 cells were treated by Hydroxyurea (4 Mm) at 37C for 20 hours or treated by Nocodazole (100 ng/mL) at 37C for 24 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/CDC2-CDK1-p-Thr14-Antibody-6R1O7-Western-Blot-NBP3-16913-img0001.jpg "Western Blot: CDC2/CDK1 [p Thr14] Antibody (6R1O7) [NBP3-16913] - Analysis of extracts of various cell lines, using CDC2/CDK1 Rabbit mAb (NBP3-16913) at 1:1000 dilution.HeLa cells and NIH/3T3 cells and C6 cells were treated by Hydroxyurea (4 Mm) at 37C for 20 hours or treated by Nocodazole (100 ng/mL) at 37C for 24 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunoprecipitation: CDC2/CDK1 Antibody [NBP3-14693] - Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells treated with Hydroxyurea (+) or mock treated (-). Antibodies:Rabbit anti-Phospho CDK1 (Y15) recombinant monoclonalantibody used for IP at 20 ul/mg lysate. Total CDK1 was immunoprecipitated byRabbit anti-CDK1 recombinant monoclonal antibody. Immunoprecipitates where treatedwith phosphatase (+) or mock treated (-). For blottingimmunoprecipitated Phospho CDK1 (Y15), wasused at 1:1000. Chemiluminescence with an exposure timeof 10 seconds. Lower panel shows WB for total CDK1 usingrabbit anti-CDK1 recombinant monoclonal.](http://images.novusbio.com/fullsize/CDC2-CDK1-Antibody-Immunoprecipitation-NBP3-14693-img0001.jpg "Immunoprecipitation: CDC2/CDK1 Antibody [NBP3-14693] - Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells treated with Hydroxyurea (+) or mock treated (-). Antibodies:Rabbit anti-Phospho CDK1 (Y15) recombinant monoclonalantibody used for IP at 20 ul/mg lysate. Total CDK1 was immunoprecipitated byRabbit anti-CDK1 recombinant monoclonal antibody. Immunoprecipitates where treatedwith phosphatase (+) or mock treated (-). For blottingimmunoprecipitated Phospho CDK1 (Y15), wasused at 1:1000. Chemiluminescence with an exposure timeof 10 seconds. Lower panel shows WB for total CDK1 usingrabbit anti-CDK1 recombinant monoclonal.")
![Western Blot:CDC2/CDK1 [p Thr14] Antibody (3V7A1)-Analysis of lysates from HeLa cells using Phospho-CDK1-T14 Rabbit mAb at 1:3000 dilution. HeLa cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/antibody/nbp3-33581_rabbit-cdc2-cdk1-p-thr14-mab-3v7a1-western-blot-171202510393..png "Western Blot:CDC2/CDK1 [p Thr14] Antibody (3V7A1)-Analysis of lysates from HeLa cells using Phospho-CDK1-T14 Rabbit mAb at 1:3000 dilution. HeLa cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 60s.")
![ELISA: Human CDC2/CDK1 ELISA Kit (Colorimetric) [NBP2-75169] - Samples were spiked with high concentrations of Human CDC2/CDK1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75169_human-cdc2-cdk1-elisa-kit-colorimetric-132202416184863.jpg "ELISA: Human CDC2/CDK1 ELISA Kit (Colorimetric) [NBP2-75169] - Samples were spiked with high concentrations of Human CDC2/CDK1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human CDC2/CDK1 ELISA Kit (Colorimetric) [NBP2-75169] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75169_human-cdc2-cdk1-elisa-kit-colorimetric-432024932692.jpg)
![Western Blot: Cdc2 Overexpression Lysate (Adult Normal) [NBL1-08991] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cdc2.](http://images.novusbio.com/fullsize/Cdc2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-08991-img0002.jpg "Western Blot: Cdc2 Overexpression Lysate (Adult Normal) [NBL1-08991] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cdc2.")
![Western Blot: CDC2/CDK1 [p Tyr15] Antibody (6P7X9) [NBP3-33570] - Western blot analysis of various lysates, using CDC2/CDK1 Rabbit mAb at1:2000 dilution. HeLa cells were treated by Hydroxyurea (4 mM) at 37C for 20 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.](http://images.novusbio.com/fullsize/nbp3-33570_rabbit-cdc2-cdk1-p-tyr15-mab-6p7x9-91220241418164.jpg "Western Blot: CDC2/CDK1 [p Tyr15] Antibody (6P7X9) [NBP3-33570] - Western blot analysis of various lysates, using CDC2/CDK1 Rabbit mAb at1:2000 dilution. HeLa cells were treated by Hydroxyurea (4 mM) at 37C for 20 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.")
![SDS-Page: Recombinant Human CDC2/CDK1 Protein [NBP2-52017] - 15% SDS Page (3 ug)](http://images.novusbio.com/fullsize/CDC2-CDK1-Recombinant-Protein-SDS-Page-NBP2-52017-img0001.jpg "SDS-Page: Recombinant Human CDC2/CDK1 Protein [NBP2-52017] - 15% SDS Page (3 ug)")
![SDS-Page: CDC2/CDK1 Recombinant Protein [NBP2-51886] - 15% SDS Page (3 ug)](http://images.novusbio.com/fullsize/CDC2-CDK1-Recombinant-Protein-SDS-Page-NBP2-51886-img0001.jpg "SDS-Page: CDC2/CDK1 Recombinant Protein [NBP2-51886] - 15% SDS Page (3 ug)")