| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62554-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48364 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-20987 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20984 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11532 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20996 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14591-500ul | |||
![Western Blot: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Western Blot using anti-CD34 antibody My10. Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of My10 (NBP2-62554) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for the canonical isoform of CD34 is 40.9kDa, though a second isoform at 35.2kDa is also present, with a 56 amino acid truncation at the C-terminus of the protein (Uniprot ID: Q64314). CD34 is also glycosylated at several positions. NBP2-62554 successfully detected both isoforms of human CD34.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Western-Blot-NBP2-62554-img0003.jpg "Western Blot: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Western Blot using anti-CD34 antibody My10. Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of My10 (NBP2-62554) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for the canonical isoform of CD34 is 40.9kDa, though a second isoform at 35.2kDa is also present, with a 56 amino acid truncation at the C-terminus of the protein (Uniprot ID: Q64314). CD34 is also glycosylated at several positions. NBP2-62554 successfully detected both isoforms of human CD34.")
![Immunohistochemistry: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Immunohistochemical staining of human placenta tissue using anti-CD34 antibody. My10 Anti-CD34 staining of paraffin embedded human placenta tissue using the rabbit-chimeric version of My10 (NBP2-62554). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-62554 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Strong endothelial staining of within villi may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Immunohistochemistry-NBP2-62554-img0002.jpg)
![Flow Cytometry: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Flow-cytometry using anti-CD34 antibody My10. Human leukocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of My10 (NBP2-62554, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Flow-Cytometry-NBP2-62554-img0001.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SPM610) - IHC-Prediluted [NBP2-48364] - Formalin-fixed, paraffin-embedded human Angiosarcoma stained with CD34 Monoclonal Antibody (SPM610).](http://images.novusbio.com/fullsize/CD34-Antibody-SPM610-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48364-img0001.jpg "Immunohistochemistry-Paraffin: CD34 Antibody (SPM610) - IHC-Prediluted [NBP2-48364] - Formalin-fixed, paraffin-embedded human Angiosarcoma stained with CD34 Monoclonal Antibody (SPM610).")
![ELISA: Human CD34 ELISA Kit (Colorimetric) [NBP2-75141] - Samples were spiked with high concentrations of Human CD34 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75141_human-cd34-elisa-kit-colorimetric-132202416235789.jpg "ELISA: Human CD34 ELISA Kit (Colorimetric) [NBP2-75141] - Samples were spiked with high concentrations of Human CD34 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human CD34 ELISA Kit (Colorimetric) [NBP2-75141] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75141_human-cd34-elisa-kit-colorimetric-432024858251.jpg)
![Western Blot: CD34 Overexpression Lysate (Adult Normal) [NBP2-08650] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CD34.](http://images.novusbio.com/fullsize/CD34-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-08650-img0001.jpg "Western Blot: CD34 Overexpression Lysate (Adult Normal) [NBP2-08650] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CD34.")
![Western Blot: CD34 Overexpression Lysate (Adult Normal) [NBP2-09777] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CD34.](http://images.novusbio.com/fullsize/CD34-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09777-img0001.jpg "Western Blot: CD34 Overexpression Lysate (Adult Normal) [NBP2-09777] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CD34.")
![Flow Cytometry: CD34 Antibody (B-G25) [NBP3-14591] - A typical staining pattern with the B-G25 monoclonal antibody of KG-1a cell line.](http://images.novusbio.com/fullsize/CD34-Antibody-B-G25-Flow-Cytometry-NBP3-14591-img0001.jpg "Flow Cytometry: CD34 Antibody (B-G25) [NBP3-14591] - A typical staining pattern with the B-G25 monoclonal antibody of KG-1a cell line.")
![Immunohistochemistry: CD34 Antibody (PDM0-12) [NBP3-32148] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-CD34 antibody (NBP3-32148) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32148) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32148_mouse-cd34-mab-pdm0-12-29820241426264.jpg "Immunohistochemistry: CD34 Antibody (PDM0-12) [NBP3-32148] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-CD34 antibody (NBP3-32148) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32148) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")