| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB90782-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1019-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-21060 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20876 | |||
![Immunohistochemistry: B7-H1/PD-L1/CD274 Antibody (2096A) [MAB90782] - B7-H1 was detected in perfusion fixed frozen sections of mouse thymus using B7-H1 Monoclonal Antibody at 1.7 ug/ml. Tissue was stained using Rabbit IgG VisUCyte HRP Polymer Antibody (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and plasma membrane.](http://images.novusbio.com/fullsize/B7-H1-PD-L1-CD274-Antibody-2096A-Immunohistochemistry-MAB90782-img0002.jpg "Immunohistochemistry: B7-H1/PD-L1/CD274 Antibody (2096A) [MAB90782] - B7-H1 was detected in perfusion fixed frozen sections of mouse thymus using B7-H1 Monoclonal Antibody at 1.7 ug/ml. Tissue was stained using Rabbit IgG VisUCyte HRP Polymer Antibody (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and plasma membrane.")
![Immunohistochemistry: PD-L1 Antibody (4V8L4) [PD-L1] - Immunohistochemistry analysis of paraffin-embedded Human placenta using PD-L1 Rabbit mAb at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15686_rabbit-pd-l1-mab-4v8l4-5220251734540.jpg "Immunohistochemistry: PD-L1 Antibody (4V8L4) [PD-L1] - Immunohistochemistry analysis of paraffin-embedded Human placenta using PD-L1 Rabbit mAb at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Immunohistochemistry: PD-L1 Antibody (4V8L4) [PD-L1] - Immunohistochemistry analysis of paraffin-embedded Human tonsil using PD-L1 Rabbit mAb at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15686_rabbit-pd-l1-mab-4v8l4-52202517315539.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: PD-L1 Antibody (4V8L4) [PD-L1] - Western blot analysis of lysates from wild type (WT) and PD-L1 knockout (KO) A-549 cells using PD-L1 Rabbit mAb at 1:1000 dilution incubated overnight at 4C. Wild type (WT) and PD-L1 knockout (KO) A549 cells were treated by IFNG (100 ng/mL) at 37C for 48 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 30 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 45s.](http://images.novusbio.com/fullsize/nbp3-15686_rabbit-pd-l1-mab-4v8l4-52202517315579.jpg)
![Immunohistochemistry: PD-L1 Antibody (4V8L4) [PD-L1] - Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma using PD-L1 Rabbit mAb at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15686_rabbit-pd-l1-mab-4v8l4-52202517311099.jpg)
![Immunohistochemistry: PD-L1 Antibody (PD01-02) [NBP3-32732] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-PD-L1 antibody (NBP3-32732) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32732) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32732_rabbit-pd-l1-mab-pd01-02-29820241492673.jpg "Immunohistochemistry: PD-L1 Antibody (PD01-02) [NBP3-32732] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-PD-L1 antibody (NBP3-32732) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32732) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: PD-L1 Antibody (PD01-02) [NBP3-32732] - Western blot analysis of PD-L1 on different lysates with Rabbit anti-PD-L1 antibody (NBP3-32732) at 1/2,000 dilution. Lane 1: MDA-MB-231 cell lysate Lane 2: MCF7 cell lysate (negative) Lane 3: U-87 MG cell lysate Lane 4: HepG2 cell lysate (low expression) Lane 5: HeLa cell lysate Lane 6: A549 cell lysate Lane 7: A549 treated with 100ng/mL IFN gamma for 48 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 33 kDa Observed band size: 45-50 kDa Exposure time: 25 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32732_rabbit-pd-l1-mab-pd01-02-3920248453543.jpg)
![Flow Cytometry: PD-L1 Antibody (29E.2A3) - Low Endotoxin, Azide and BSA Free [NBP3-09098] - Separation of cells stained using anti-humam CD274 (29E.2A3) purified antibody (low endotoxin, concentration in sample 4 ug/ml, GAM APC, red-filled) from cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining) of PHA stimulated peripheral blood mononuclear cell suspension.](http://images.novusbio.com/fullsize/PD-L1-Antibody-29E-2A3-Low-Endotoxin-Azide-and-BSA-Free-Flow-Cytometry-NBP3-09098-img0002.jpg "Flow Cytometry: PD-L1 Antibody (29E.2A3) - Low Endotoxin, Azide and BSA Free [NBP3-09098] - Separation of cells stained using anti-humam CD274 (29E.2A3) purified antibody (low endotoxin, concentration in sample 4 ug/ml, GAM APC, red-filled) from cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining) of PHA stimulated peripheral blood mononuclear cell suspension.")
![Flow Cytometry: PD-L1 Antibody (29E.2A3) - Low Endotoxin, Azide and BSA Free [NBP3-09098] - Surface staining pattern of human PHA stimulated peripheral blood mononuclear cell suspension stained using anti-humam CD274 (29E.2A3) purified antibody (low endotoxin, concentration in sample 4 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/PD-L1-Antibody-29E-2A3-Low-Endotoxin-Azide-and-BSA-Free-Flow-Cytometry-NBP3-09098-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PD-L1 Antibody (F2G2) - VHH [NBP3-12826] - Immunofluorescence of PD-L1 in transfected 293 cells with PD-L1 antibody at 10 ug/ml. Green: PD-L1 Antibody [F2G2] Blue: DAPI staining](http://images.novusbio.com/fullsize/PD-L1-Antibody-F2G2-VHH-Immunocytochemistry-Immunofluorescence-NBP3-12826-img0002.jpg "Immunocytochemistry/Immunofluorescence: PD-L1 Antibody (F2G2) - VHH [NBP3-12826] - Immunofluorescence of PD-L1 in transfected 293 cells with PD-L1 antibody at 10 ug/ml. Green: PD-L1 Antibody [F2G2] Blue: DAPI staining")
![Immunocytochemistry/Immunofluorescence: PD-L1 Antibody (F2G2) - VHH [NBP3-12826] - Immunocytochemistry of PD-L1 in transfected 293 cells with PD-L1 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/PD-L1-Antibody-F2G2-VHH-Immunocytochemistry-Immunofluorescence-NBP3-12826-img0003.jpg)
![ELISA: PD-L1 Antibody (F2G2) - VHH [NBP3-12826] - Titration ELISA of PD-L1.](http://images.novusbio.com/fullsize/PD-L1-Antibody-F2G2-VHH-ELISA-NBP3-12826-img0001.jpg)
