CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and plasma membranes of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="BRCA1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Sheep Anti-Human BRCA1 C-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6955) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and plasma membranes of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF6955-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54305-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-62628-25ul | |||
![Western Blot: BRCA1 Antibody [NB100-199] - Whole cell lysate (50 ug) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRCA1 antibody used for WB at 0.04ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds](http://images.novusbio.com/fullsize/BRCA1-Antibody-Western-Blot-NB100-199-img0004.jpg "Western Blot: BRCA1 Antibody [NB100-199] - Whole cell lysate (50 ug) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRCA1 antibody used for WB at 0.04ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds")
![Western Blot: BRCA1 Antibody [NB100-199] - Detection of Human BRCA1 by Western Blot and Immunoprecipitation. Sample: Nuclear extract from HeLa cells. Affinity purified rabbit anti-BRCA1 NB100-199 used at 1 mcg/ml for WB and 4 mcg for IP.](http://images.novusbio.com/fullsize/BRCA1-Antibody-Western-Blot-NB100-199-img0003.jpg)
![Immunohistochemistry-Paraffin: BRCA1 Antibody (BRCA1/1398) [NBP2-53251] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with BRCA1 Monoclonal Antibody (BRCA1/1398).](http://images.novusbio.com/fullsize/BRCA1-Antibody-BRCA1-1398-Immunohistochemistry-Paraffin-NBP2-53251-img0001.jpg "Immunohistochemistry-Paraffin: BRCA1 Antibody (BRCA1/1398) [NBP2-53251] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with BRCA1 Monoclonal Antibody (BRCA1/1398).")
![Protein Array: BRCA1 Antibody (BRCA1/1398) [NBP2-53251] - Analysis of Protein Array containing more than 19,000 full-length human proteins using BRCA1 Antibody (BRCA1/1398). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/BRCA1-Antibody-BRCA1-1398-Protein-Array-NBP2-53251-img0002.jpg)
![SDS-Page: Recombinant Human BRCA1 Protein [H00000672-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-BRCA1-Protein-SDS-Page-H00000672-P01-img0001.jpg "SDS-Page: Recombinant Human BRCA1 Protein [H00000672-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunohistochemistry-Paraffin: BRCA1 Antibody [NBP2-62628] - Immunohistochemistry analysis in human testis and pancreas tissues using Anti-BRCA1 antibody. Corresponding BRCA1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/BRCA1-Antibody-Immunohistochemistry-Paraffin-NBP2-62628-img0001.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: BRCA1 Antibody [NBP2-62628] - Immunohistochemistry analysis in human testis and pancreas tissues using Anti-BRCA1 antibody. Corresponding BRCA1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: BRCA1 Antibody [NBP2-62628] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/BRCA1-Antibody-Immunohistochemistry-Paraffin-NBP2-62628-img0002.jpg)
![Immunohistochemistry-Paraffin: BRCA1 Antibody [NBP2-62628] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/BRCA1-Antibody-Immunohistochemistry-Paraffin-NBP2-62628-img0003.jpg)
![Immunohistochemistry: BRCA1 Antibody (A7E3-R) [NBP3-32090] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-BRCA1 antibody (NBP3-32090) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32090) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32090_mouse-brca1-mab-a7e3-r-298202414304150.jpg "Immunohistochemistry: BRCA1 Antibody (A7E3-R) [NBP3-32090] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-BRCA1 antibody (NBP3-32090) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32090) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: BRCA1 [p Ser1280] Antibody [NB100-224] - Detection of Brca1 phospho Ser 1280 by Western Blot using Rabbit anti-Brca1 pSer1280 (cat. no. NB100-224).](http://images.novusbio.com/fullsize/BRCA1-[p-Ser1280]-Antibody-Western-Blot-NB100-224-img0001.jpg "Western Blot: BRCA1 [p Ser1280] Antibody [NB100-224] - Detection of Brca1 phospho Ser 1280 by Western Blot using Rabbit anti-Brca1 pSer1280 (cat. no. NB100-224).")
![Western Blot: BRCA1 [p Ser1189] Antibody [NB100-229] - Detection of Brca1 Phosph Ser 1189 by Western Blot using Rabbit anti-BRCA1 [p Ser1189] Antibody.](http://images.novusbio.com/fullsize/BRCA1-[p-Ser1189]-Antibody-Western-Blot-NB100-229-img0001.jpg "Western Blot: BRCA1 [p Ser1189] Antibody [NB100-229] - Detection of Brca1 Phosph Ser 1189 by Western Blot using Rabbit anti-BRCA1 [p Ser1189] Antibody.")
![Western Blot: BRCA1 [p Ser1466] Antibody [NB100-228] - Whole Cell Lysate (25ug/lane) from Hela cells treated with 10 Gy IR or untreated. Antibody diluted 1:2000](http://images.novusbio.com/fullsize/BRCA1-[p-Ser1466]-Antibody-Western-Blot-NB100-228-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: BRCA1 [p Ser1466] Antibody [NB100-228] - Whole Cell Lysate (25ug/lane) from Hela cells treated with 10 Gy IR or untreated. Antibody diluted 1:2000")
![SDS-Page: Recombinant Human BRCA1 Protein [H00000672-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-BRCA1-Protein-SDS-Page-H00000672-Q01-img0001.jpg "SDS-Page: Recombinant Human BRCA1 Protein [H00000672-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
