HAF016). A specific band was detected for CD79A at approximately 32-35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse CD79A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6968) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD79A at approximately 32-35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF6968-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47839-0.1mg | |||
| IHC-Prediluted | NBP2-48030 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-21054 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54370-100ug | |||
![Immunohistochemistry-Paraffin: CD79A Antibody (IGA/515) [NBP2-44742] - Formalin-fixed, paraffin-embedded human Lymphoma stained with CD79A Antibody (IGA/515).](http://images.novusbio.com/fullsize/CD79A-Antibody-IGA-515-Immunohistochemistry-Paraffin-NBP2-44742-img0003.jpg "Immunohistochemistry-Paraffin: CD79A Antibody (IGA/515) [NBP2-44742] - Formalin-fixed, paraffin-embedded human Lymphoma stained with CD79A Antibody (IGA/515).")
![Western Blot: CD79A Antibody (PDM0-23) [NBP3-32168] - Western blot analysis of CD79A on Raji cell lysates with Mouse anti-CD79A antibody (NBP3-32168) at 1/1,000 dilution.<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 25 kDa<br/> Observed band size: 40 kDa<br/><br/> Exposure time: 2 minutes;<br/><br/> 12% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32168) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32168_mouse-cd79a-mab-pdm0-23-278202415295589.jpg "Western Blot: CD79A Antibody (PDM0-23) [NBP3-32168] - Western blot analysis of CD79A on Raji cell lysates with Mouse anti-CD79A antibody (NBP3-32168) at 1/1,000 dilution.<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 25 kDa<br/> Observed band size: 40 kDa<br/><br/> Exposure time: 2 minutes;<br/><br/> 12% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32168) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD79A Antibody (PDM0-23) [NBP3-32168] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD79A antibody (NBP3-32168) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32168) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32168_mouse-cd79a-mab-pdm0-23-29820241435290.jpg)
![Western blot analysis on different lysates with NBP3-32168 at 1/2,000 dilution. Lane 1: Daudi (Human Burkitt's lymphoma cell) cell lysate Lane 2: Ramos (Human Burkitt's lymphoma cell) cell lysate Lane 3: Raji (Human Burkitt's lymphoma cell) cell lysate Lane 4: Jurkat (Human T-lymphoblastic cells) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 46 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: NBP3-32168, 1/2,000 in primary antibody dilution buffer, overnight at 4 ℃ Secondary antibody: Goat Anti-Rabbit IgG [HRP] Secondary Antibody, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 25 kDa Observed band size: 45-55 kDa](http://images.novusbio.com/fullsize/nbp3-32168_-western-blot-639204590074298602.jpg)
![Western Blot: CD79A Antibody (IGA/1406) [NBP2-53254] - Western Blot of Ramos Cell Lysate using CD79a Monoclonal Antibody (IGA/1406).](http://images.novusbio.com/fullsize/CD79A-Antibody-IGA-1406-Western-Blot-NBP2-53254-img0001.jpg "Western Blot: CD79A Antibody (IGA/1406) [NBP2-53254] - Western Blot of Ramos Cell Lysate using CD79a Monoclonal Antibody (IGA/1406).")
![Western Blot: CD79A Antibody (S02-7E3) [NBP3-19601] - Western blot detection of CD79A in Jurkat cell lysates using NBP3-19601 (1:500 diluted). Predicted band size: 25kDa. Observed band size: 45-55kDa.](http://images.novusbio.com/fullsize/CD79A-Antibody-S02-7E3-Western-Blot-NBP3-19601-img0002.jpg "Western Blot: CD79A Antibody (S02-7E3) [NBP3-19601] - Western blot detection of CD79A in Jurkat cell lysates using NBP3-19601 (1:500 diluted). Predicted band size: 25kDa. Observed band size: 45-55kDa.")
![Immunohistochemistry-Paraffin: CD79A Antibody (S02-7E3) [NBP3-19601] - Immunohistochemistry of CD79A in paraffin-embedded Human tonsil using NBP3-19601 at dilution 1/50](http://images.novusbio.com/fullsize/CD79A-Antibody-S02-7E3-Immunohistochemistry-Paraffin-NBP3-19601-img0001.jpg)
