BAF632) were stained with the indicated concentrations of Recombinant Human CD40/TNFRSF5 Fc Chimera His-tag Alexa Fluor® 488 (Catalog # AFG1493). " alt="Streptavidin coated beads conjugated to Human CD40/TNFRSF5 Biotinylated Antibody (BAF632) were stained with the indicated concentrations of Recombinant Human CD40/TNFRSF5 Fc Chimera His-tag Alexa Fluor® 488 (Catalog # AFG1493). " class="big_thumb" />
6420-CL) is immobilized at 2 µg/mL, 100 µL/well, the concentration of Biotinylated Recombinant Human CD40/TNFRSF5 Fc Chimera Avi-tag (Catalog # AVI10380) that produces 50% optimal binding is 0.0400-0.320 μg/mL." alt="When Recombinant Human CD40 Ligand/TNFSF5 (HEK293-expressed) Protein (Catalog # 6420-CL) is immobilized at 2 µg/mL, 100 µL/well, the concentration of Biotinylated Recombinant Human CD40/TNFRSF5 Fc Chimera Avi-tag (Catalog # AVI10380) that produces 50% optimal binding is 0.0400-0.320 μg/mL." class="big_thumb" />
BAF632) were stained with the indicated concentrations of Recombinant Human CD40/TNFRSF5 Fc Chimera His-tag Alexa Fluor® 647 (Catalog # AFR1493). " alt="Streptavidin coated beads conjugated to Human CD40/TNFRSF5 Biotinylated Antibody (BAF632) were stained with the indicated concentrations of Recombinant Human CD40/TNFRSF5 Fc Chimera His-tag Alexa Fluor® 647 (Catalog # AFR1493). " class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81104-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52648-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81101-0.025mg | |||
6420-CL) binds with an ED50 of 5-25 ng/mL." alt="When Recombinant Cynomolgus Monkey CD40/TNFRSF5 Fc Chimera (Catalog # 9660-CD) is immobilized at 1 µg/mL, 100 µL/well, Recombinant Human CD40 Ligand/TNFSF5 (Catalog # 6420-CL) binds with an ED50 of 5-25 ng/mL." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50284-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52697-0.025mg | |||
![Flow Cytometry: CD40/TNFRSF5 Antibody (G28.5) - Chimeric [NBP2-52648] - Flow-cytometry using anti-CD40 antibody G28.5. Cynomolgus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of G28.5 (NBP2-52648, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-G28-5-Chimeric-Flow-Cytometry-NBP2-52648-img0003.jpg "Flow Cytometry: CD40/TNFRSF5 Antibody (G28.5) - Chimeric [NBP2-52648] - Flow-cytometry using anti-CD40 antibody G28.5. Cynomolgus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of G28.5 (NBP2-52648, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.")
![Flow Cytometry: CD40/TNFRSF5 Antibody (G28.5) - Chimeric [NBP2-52648] - Flow-cytometry using anti-CD40 antibody G28.5. Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of G28.5 (NBP2-52648, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-G28-5-Chimeric-Flow-Cytometry-NBP2-52648-img0001.jpg)
![Flow Cytometry: CD40/TNFRSF5 Antibody (G28.5) - Chimeric [NBP2-52648] - Flow-cytometry using anti-CD40 antibody G28.5. Rhesus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of G28.5 (NBP2-52648, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-G28-5-Chimeric-Flow-Cytometry-NBP2-52648-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD40/TNFRSF5 Antibody (A54 scFv) [NBP2-81101] - Immunofluorescence staining of fixed Daudi cells with [NBP2-81101]. Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of A54 scFv [NBP2-81101] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81101, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-A54-scFv-Immunocytochemistry-Immunofluorescence-NBP2-81101-img0001.jpg "Immunocytochemistry/Immunofluorescence: CD40/TNFRSF5 Antibody (A54 scFv) [NBP2-81101] - Immunofluorescence staining of fixed Daudi cells with [NBP2-81101]. Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of A54 scFv [NBP2-81101] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81101, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.")
![Western Blot: CD40/TNFRSF5 Antibody (T8P2G4/A6) [NBP2-50284] - Analysis of CD40 in Jurkat cell lysates.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-T8P2G4-A6-Western-Blot-NBP2-50284-img0001.jpg "Western Blot: CD40/TNFRSF5 Antibody (T8P2G4/A6) [NBP2-50284] - Analysis of CD40 in Jurkat cell lysates.")
![Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody (T8P2G4/A6) [NBP2-50284] - Analysis of CD40 in a hepatocellular carcinoma tissue cross section.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-T8P2G4-A6-Immunohistochemistry-Paraffin-NBP2-50284-img0002.jpg)
![Immunohistochemistry: CD40/TNFRSF5 Antibody (T8P2G4/A6) [NBP2-50284] - Analysis of CD40 in a cross section of human breast cancer tissue. Positive cytoplasmic staining is shown.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-T8P2G4-A6-Immunohistochemistry-NBP2-50284-img0003.jpg)
![Western Blot: CD40 Overexpression Lysate (Adult Normal) [NBL1-08948] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CD40.](http://images.novusbio.com/fullsize/CD40-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-08948-img0002.jpg "Western Blot: CD40 Overexpression Lysate (Adult Normal) [NBL1-08948] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CD40.")
