MAB0041, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins." alt="U937 Human histiocytic lymphoma cell line was stained with Mouse Anti-Human Siglec-3/CD33 Monoclonal Antibody (Catalog # MAB11374, filled histogram) or Mouse IgG2B isotype control antibody (Catalog # MAB0041, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB11374-SP | |||
| Carrier Free | FAB11374V-100UG | |||
| Carrier Free | FAB11374U-100UG | |||
| Carrier Free | FAB11374T-100UG | |||
| Carrier Free | FAB11374S-100UG | |||
| Carrier Free | FAB11374R-100UG | |||
| Carrier Free | FAB11374N-100UG | |||
| Carrier Free | FAB11374G-100UG | |||
MAB1137) were stained with (A) Recombinant Human Siglec 3/CD33 Protein, Atto 488 Conjugate (Catalog # ATJ1137) or (B) unstained." alt="Fluorescent beads conjugated to anti-Human Siglec 3/CD33 Monoclonal Antibody (MAB1137) were stained with (A) Recombinant Human Siglec 3/CD33 Protein, Atto 488 Conjugate (Catalog # ATJ1137) or (B) unstained." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2220-SP | |||
AF011), and then Allophycocyanin-conjugated Anti-Goat IgG Tertiary Antibody (F0108). Quadrant markers were set based on secondary plus tertiary antibody staining in the absence of primary antibody. View our protocol for Staining Membrane-associated Proteins. " alt="HEK293 human embryonic kidney cell line transfected with either (A) human Siglec-3/CD33 or (B) irrelevant protein and eGFP was stained with Llama Anti-Human Siglec-3/CD33 Llamabody Native IgG Monoclonal Antibody (Catalog # LMAB10902) followed by Goat Anti-Llama Secondary Antibody (AF011), and then Allophycocyanin-conjugated Anti-Goat IgG Tertiary Antibody (F0108). Quadrant markers were set based on secondary plus tertiary antibody staining in the absence of primary antibody. View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | LMAB10902-SP | |||
F0135). Staining was performed using our Staining Membrane-associated Proteins protocol." alt="HEK293 human embryonic kidney cell line transfected with either (A) human Siglec-3/CD33 or (B) irrelevant protein and eGFP was stained with Llama Anti-Human Siglec-3/CD33 Llamabody VHH hIgG2 Monoclonal Antibody (Catalog # LMAB10888) followed by Allophycocyanin-conjugated Goat Anti-Human Secondary Antibody (F0135). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | LMAB10888-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-75905SS | |||
![Western Blot: Siglec-3/CD33 Antibody (BLR061G) [NBP2-77356] - Antibody: Rabbit anti-CD33 recombinant monoclonal [BLR061G] (NBP2-77356). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-GAPDH.](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-BLR061G-Western-Blot-NBP2-77356-img0002.jpg "Western Blot: Siglec-3/CD33 Antibody (BLR061G) [NBP2-77356] - Antibody: Rabbit anti-CD33 recombinant monoclonal [BLR061G] (NBP2-77356). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-GAPDH.")
![Immunohistochemistry-Paraffin: Siglec-3/CD33 Antibody (BLR061G) [NBP2-77356] - Section of Acute Myeloid Leukemia (AML). Antibody: Rabbit anti-CD33 recombinant monoclonal antibody [BLR061G] (A700-061 lot 1) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-BLR061G-Immunohistochemistry-Paraffin-NBP2-77356-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Siglec-3/CD33 Antibody (BLR061G) [NBP2-77356] - Detection of human CD33 by WB of immunoprecipitates from HEL 92.1.7 lysate. Antibodies: Rabbit anti-CD33 recombinant monoclonal [BLR061G] (NBP2-77356) and rabbit anti-CD33 recombinant monoclonal [BL-302-3E10].](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-BLR061G-Immunoprecipitation-NBP2-77356-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: Siglec-3/CD33 Antibody (PSH02-50) [NBP3-32146] - Immunocytochemistry analysis of TF-1 (positive) and Jurkat (negative) labeling Siglec-3/CD33 with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32146) at 1/200 dilution.<br/><br/> Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32146) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32146_rabbit-siglec-3-cd33-mab-psh02-50-29202410484839.jpg "Immunocytochemistry/ Immunofluorescence: Siglec-3/CD33 Antibody (PSH02-50) [NBP3-32146] - Immunocytochemistry analysis of TF-1 (positive) and Jurkat (negative) labeling Siglec-3/CD33 with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32146) at 1/200 dilution.<br/><br/> Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32146) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.")
![Flow Cytometry: Siglec-3/CD33 Antibody (PSH02-50) [NBP3-32146] - Flow cytometric analysis of TF-1 cells labeling Siglec-3/CD33.<br/><br/> Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32146, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32146_rabbit-siglec-3-cd33-mab-psh02-50-29202416453211.jpg)
![Western blot analysis on different lysates with NBP3-32146 at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: TF-1 cell lysate Lane 3: HL-60 cell lysate Lane 4: Jurkat cell lysate (negative) Lane 5: SK-MEL-28 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 40 kDa Observed band size: 70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32146 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32146_-western-blot-639204597140781013.jpg)
![Western blot analysis on different lysates with NBP3-32146 at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 cell lysate treated with deglycosylation Predicted band size: 40 kDa Observed band size: 40/70 kDa (Glycosylated) Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32146 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32146_-western-blot-639204598085420546.jpg)
![Immunocytochemistry/ Immunofluorescence: Siglec-3/CD33 Antibody (PSH02-51) [NBP3-32147] - Immunocytochemistry analysis of TF-1 (positive) and Jurkat (negative) labeling Siglec-3/CD33 with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32147) at 1/500 dilution.<br/><br/> Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32147) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32147_rabbit-siglec-3-cd33-mab-psh02-51-29202410334035.jpg "Immunocytochemistry/ Immunofluorescence: Siglec-3/CD33 Antibody (PSH02-51) [NBP3-32147] - Immunocytochemistry analysis of TF-1 (positive) and Jurkat (negative) labeling Siglec-3/CD33 with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32147) at 1/500 dilution.<br/><br/> Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32147) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.")
![Flow Cytometry: Siglec-3/CD33 Antibody (PSH02-51) [NBP3-32147] - Flow cytometric analysis of TF-1 cells labeling Siglec-3/CD33.<br/><br/> Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32147, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32147_rabbit-siglec-3-cd33-mab-psh02-51-29202416453237.jpg)
![Western blot analysis on different lysates with NBP3-32147 at 1/2,000 dilution. Lane 1: TF-1 cell lysate Lane 2: Jurkat cell lysate (negative) Lane 3: SK-MEL-28 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 40 kDa Observed band size: 70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32147 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32147_-western-blot-639204597140821925.jpg)
![Western Blot: CD33 Overexpression Lysate (Adult Normal) [NBP2-04204] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CD33.](http://images.novusbio.com/fullsize/CD33-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04204-img0001.jpg "Western Blot: CD33 Overexpression Lysate (Adult Normal) [NBP2-04204] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CD33.")
