| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2220-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-32709-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | LMAB10888-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | LMAB10902-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34511-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50292-0.025ml | |||
![Western Blot: Siglec-3/CD33 Antibody (BLR061G) [NBP2-77356] - Antibody: Rabbit anti-CD33 recombinant monoclonal [BLR061G] (NBP2-77356). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-GAPDH.](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-BLR061G-Western-Blot-NBP2-77356-img0002.jpg "Western Blot: Siglec-3/CD33 Antibody (BLR061G) [NBP2-77356] - Antibody: Rabbit anti-CD33 recombinant monoclonal [BLR061G] (NBP2-77356). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-GAPDH.")
![Immunohistochemistry-Paraffin: Siglec-3/CD33 Antibody (BLR061G) [NBP2-77356] - Section of Acute Myeloid Leukemia (AML). Antibody: Rabbit anti-CD33 recombinant monoclonal antibody [BLR061G] (A700-061 lot 1) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-BLR061G-Immunohistochemistry-Paraffin-NBP2-77356-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Siglec-3/CD33 Antibody (BLR061G) [NBP2-77356] - Detection of human CD33 by WB of immunoprecipitates from HEL 92.1.7 lysate. Antibodies: Rabbit anti-CD33 recombinant monoclonal [BLR061G] (NBP2-77356) and rabbit anti-CD33 recombinant monoclonal [BL-302-3E10].](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-BLR061G-Immunoprecipitation-NBP2-77356-img0003.jpg)
![Immunohistochemistry-Paraffin: Siglec-3/CD33 Antibody [NBP2-32709] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-Immunohistochemistry-Paraffin-NBP2-32709-img0014.jpg "Immunohistochemistry-Paraffin: Siglec-3/CD33 Antibody [NBP2-32709] - Staining of human skeletal muscle shows no positivity in myocytes as expected.")
![Immunohistochemistry-Paraffin: Siglec-3/CD33 Antibody [NBP2-32709] - Staining of human lung shows moderate positivity in macrophages.](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-Immunohistochemistry-Paraffin-NBP2-32709-img0011.jpg)
![Immunohistochemistry-Paraffin: Siglec-3/CD33 Antibody [NBP2-32709] - Staining of human lymph node shows moderate positivity in lymphoid cells.](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-Immunohistochemistry-Paraffin-NBP2-32709-img0012.jpg)
![Immunohistochemistry-Paraffin: Siglec-3/CD33 Antibody [NBP2-32709] - Staining of human placenta shows moderate positivity in lymphoid cells.](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-Immunohistochemistry-Paraffin-NBP2-32709-img0013.jpg)
![Western Blot: Siglec-3/CD33 Antibody (PSH02-51) [NBP3-32147] - Western blot analysis of Siglec-3/CD33 on different lysates with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32147) at 1/2,000 dilution. Lane 1: TF-1 cell lysate Lane 2: Jurkat cell lysate (negative) Lane 3: SK-MEL-28 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 40 kDa Observed band size: 70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32147) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32147_rabbit-siglec-3-cd33-mab-psh02-51-278202415212472.jpg "Western Blot: Siglec-3/CD33 Antibody (PSH02-51) [NBP3-32147] - Western blot analysis of Siglec-3/CD33 on different lysates with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32147) at 1/2,000 dilution. Lane 1: TF-1 cell lysate Lane 2: Jurkat cell lysate (negative) Lane 3: SK-MEL-28 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 40 kDa Observed band size: 70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32147) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Siglec-3/CD33 Antibody (PSH02-51) [NBP3-32147] - Immunocytochemistry analysis of TF-1 (positive) and Jurkat (negative) labeling Siglec-3/CD33 with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32147) at 1/500 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32147) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32147_rabbit-siglec-3-cd33-mab-psh02-51-29202410334035.jpg)
![Flow Cytometry: Siglec-3/CD33 Antibody (PSH02-51) [NBP3-32147] - Flow cytometric analysis of TF-1 cells labeling Siglec-3/CD33. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32147, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32147_rabbit-siglec-3-cd33-mab-psh02-51-29202416453237.jpg)
![Western Blot: Siglec-3/CD33 Antibody (PSH02-50) [NBP3-32146] - Western blot analysis of Siglec-3/CD33 on different lysates with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32146) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: TF-1 cell lysate Lane 3: HL-60 cell lysate Lane 4: Jurkat cell lysate (negative) Lane 5: SK-MEL-28 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 40 kDa Observed band size: 70 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32146) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32146_rabbit-siglec-3-cd33-mab-psh02-50-278202415212431.jpg "Western Blot: Siglec-3/CD33 Antibody (PSH02-50) [NBP3-32146] - Western blot analysis of Siglec-3/CD33 on different lysates with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32146) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: TF-1 cell lysate Lane 3: HL-60 cell lysate Lane 4: Jurkat cell lysate (negative) Lane 5: SK-MEL-28 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 40 kDa Observed band size: 70 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32146) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Siglec-3/CD33 Antibody (PSH02-50) [NBP3-32146] - Immunocytochemistry analysis of TF-1 (positive) and Jurkat (negative) labeling Siglec-3/CD33 with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32146) at 1/200 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32146) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32146_rabbit-siglec-3-cd33-mab-psh02-50-29202410484839.jpg)
![Flow Cytometry: Siglec-3/CD33 Antibody (PSH02-50) [NBP3-32146] - Flow cytometric analysis of TF-1 cells labeling Siglec-3/CD33. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32146, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32146_rabbit-siglec-3-cd33-mab-psh02-50-29202416453211.jpg)
![Flow Cytometry: Siglec-3/CD33 Antibody (HIM3-4) [NB500-508] - Using the Biotin direct conjugate Surface staining of human peripheral blood leukocytes with anti-human CD33 (HIM3-4) biotin / streptavidin-APC.](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-HIM3-4-Flow-Cytometry-NB500-508-img0003.jpg "Flow Cytometry: Siglec-3/CD33 Antibody (HIM3-4) [NB500-508] - Using the Biotin direct conjugate Surface staining of human peripheral blood leukocytes with anti-human CD33 (HIM3-4) biotin / streptavidin-APC.")
![Flow Cytometry: Siglec-3/CD33 Antibody (HIM3-4) [NB500-508] - Analysis using the PE/Cy5 conjugate of NB500-508. Staining of normal human peripheral blood cells with PE-Cy5 Mouse IgG1 K Isotype Control (open histogram) or Anti-Human CD33 PE-Cy5 (filled histogram). Cells in the monocyte gate were used for analysis.](http://images.novusbio.com/fullsize/Siglec-3-CD33-Antibody-HIM3-4-Flow-Cytometry-NB500-508-img0002.jpg)