Products Related to Gene-ID: 942

HAF018). A specific band was detected for B7‑2/CD86 at approximately 70 kDa (as indicated) in the parental cell line, but is not detectable in knockout cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for B7‑2/CD86 at approximately 70 kDa (as indicated) in the parental cell line, but is not detectable in knockout cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows lysates of Ramos human Burkitt's lymphoma cell line and human B7-2/CD86 knockout Ramos cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Cynomolgus Monkey B7‑2/CD86 Monoclonal Antibody (Catalog # MAB10601) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for B7‑2/CD86 at approximately 70 kDa (as indicated) in the parental cell line, but is not detectable in knockout cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

HAF018). A specific band was detected for B7‑2/CD86 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for B7‑2/CD86 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

7268-CT) is immobilized at 0.25 µg/mL, Recombinant Human B7-2/CD86 Fc Chimera Avi-tag (Catalog # AVI7625) binds with an ED₅₀ of 5-30 ng/mL." class="big_lightbox"> 7268-CT) is immobilized at 0.25 µg/mL, Recombinant Human B7-2/CD86 Fc Chimera Avi-tag (Catalog # AVI7625) binds with an ED₅₀ of 5-30 ng/mL." alt="When Recombinant Human CTLA-4 Fc Chimera (Catalog # 7268-CT) is immobilized at 0.25 µg/mL, Recombinant Human B7-2/CD86 Fc Chimera Avi-tag (Catalog # AVI7625) binds with an ED₅₀ of 5-30 ng/mL." class="big_thumb" />

7268-CT) binds with an ED₅₀ of 5-25 ng/mL." class="big_lightbox"> 7268-CT) binds with an ED₅₀ of 5-25 ng/mL." alt="When Recombinant Cynomolgus Monkey B7-2/CD86 His-tag Protein (Catalog # 10300-B2) is coated at 0.5 µg/mL, 100 µL/well, Recombinant Human CTLA-4 Fc Chimera (Catalog # 7268-CT) binds with an ED₅₀ of 5-25 ng/mL." class="big_thumb" />

VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="B7‑2/CD86 was detected in immersion fixed paraffin-embedded sections of mouse spleen using Rat Anti-Mouse B7‑2/CD86 Monoclonal Antibody (Catalog # mab11469) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />